##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AccIII AgeI AscI
AseI AsnI AviII AvrII BbrPI
BcgI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BseRI BsiWI BsmI Bsp1407I BspDI
BspEI BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I EcoT22I FbaI
FseI FspI HpaI KspI MamI
MfeI MroI MunI NaeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3224 GGTACC G^GTACC
AclI 392 AACGTT AA^CGTT
AcyI 1103 GGCGCC G[GA]^CG[CT]C
AflII 2753 CTTAAG C^TTAAG
AhdI 3068 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 412 AGCGCT AGC^GCT
ApaI 34 GGGCCC GGGCC^C
Asp718I 3224 GGTACC G^GTACC
AspEI 3068 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3035 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1136 GGATCC G^GATCC
BbeI 1103 GGCGCC GGCGC^C
BciVI 933 GTATCC GTATCC(6/5)
BfrI 2753 CTTAAG C^TTAAG
BsaHI 1103 GGCGCC G[GA]^CG[CT]C
BsaWI 2928 ACCGGA [AT]^CCGG[AT]
BsiEI 1577 CGGTCG CG[GA][CT]^CG
BsmBI 795 CGTCTC CGTCTC(1/5)
BspLU11I 3863 ACATGT A^CATGT
BspMI 1187 GCAGGT ACCTGC(4/8)
BssHII 114 GCGCGC G^CGCGC
BstXI 2198 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2709 TTTAAA TTT^AAA
DraIII 1095 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3068 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 412 AGCGCT AGC^GCT
EcoNI 583 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2418 GATATC GAT^ATC
Hin1I 1103 GGCGCC G[GA]^CG[CT]C
KasI 1103 GGCGCC G^GCGCC
KpnI 3224 GGTACC GGTAC^C
MluI 2024 ACGCGT A^CGCGT
NarI 1103 GGCGCC GG^CGCC
NdeI 1867 CATATG CA^TATG
NruI 58 TCGCGA TCG^CGA
NspI 3863 ACATGT [GA]CATG^[CT]
PflFI 3035 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 392 AACGTT AA^CGTT
SacI 1056 GAGCTC GAGCT^C
SapI 1400 GAAGAGC GCTCTTC(1/4)
SexAI 1507 ACCTGGT A^CC[AT]GGT
SfoI 1103 GGCGCC GGC^GCC
SmaI 451 CCCGGG CCC^GGG
Tth111I 3035 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1042 CCAGGGACAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 451 CCCGGG C^CCGGG
XmaI 451 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 2024 ACGCGT A^C[GA][CT]GT
3863 ACATGT
AocI 1131 CCTCAGG CC^T[ACGT]AGG
3547 CCTTAGG
BalI 95 TGGCCA TGG^CCA
2522 TGGCCA
BanII 34 GGGCCC G[GA]GC[CT]^C
1056 GAGCTC
BbvCI 3228 CCTCAGC CCTCAGC(-5/-2)
494 GCTGAGG
BspHI 2585 TCATGA T^CATGA
3115 TCATGA
BsrFI 101 ACCGGC [GA]^CCGG[CT]
3466 GCCGGT
Bsu36I 1131 CCTCAGG CC^T[ACGT]AGG
3547 CCTTAGG
Cfr10I 101 ACCGGC [GA]^CCGG[CT]
3466 GCCGGT
Eco81I 1131 CCTCAGG CC^T[ACGT]AGG
3547 CCTTAGG
HgaI 1441 GCGTC GACGC(5/10)
2050 GCGTC
HindIII 1928 AAGCTT A^AGCTT
3134 AAGCTT
MluNI 95 TGGCCA TGG^CCA
2522 TGGCCA
MscI 95 TGGCCA TGG^CCA
2522 TGGCCA
NcoI 1027 CCATGG C^CATGG
3026 CCATGG
PflMI 119 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
893 CCAGAAGTTGG
PstI 737 CTGCAG CTGCA^G
3060 CTGCAG
RcaI 2585 TCATGA T^CATGA
3115 TCATGA
SspI 1693 AATATT AAT^ATT
3974 AATATT
Van91I 119 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
893 CCAGAAGTTGG
XbaI 3008 TCTAGA T^CTAGA
3246 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BanI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI