##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BciVI BfrI BlnI
BsaBI Bsp1407I BspDI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EcoO65I EcoRV FseI HindIII HpaI
KpnI MamI MluI NdeI NheI
NotI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SapI ScaI SfiI SfuI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 398 TCCGGA T^CCGGA
AgeI 110 ACCGGT A^CCGGT
Alw44I 533 GTGCAC G^TGCAC
Aor51HI 1154 AGCGCT AGC^GCT
ApaLI 533 GTGCAC G^TGCAC
AscI 172 GGCGCGCC GG^CGCGCC
AviII 1031 TGCGCA TGC^GCA
BbsI 1263 GAAGAC GAAGAC(2/6)
BcgI 493 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2710 TGATCA T^GATCA
BlpI 2316 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2316 GCTGAGC GC^T[ACGT]AGC
BpuAI 1263 GAAGAC GAAGAC(2/6)
BseAI 398 TCCGGA T^CCGGA
BsiWI 882 CGTACG C^GTACG
BsmBI 1789 GAGACG CGTCTC(1/5)
BspEI 398 TCCGGA T^CCGGA
BspHI 1300 TCATGA T^CATGA
BssSI 1062 CACGAG CACGAG(-5/-1)
BstXI 2845 CCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2316 GCTGAGC GC^T[ACGT]AGC
Eco47III 1154 AGCGCT AGC^GCT
EcoRI 1044 GAATTC G^AATTC
FbaI 2710 TGATCA T^GATCA
FspI 1031 TGCGCA TGC^GCA
MfeI 1441 CAATTG C^AATTG
MroI 398 TCCGGA T^CCGGA
MseI 2809 TTAA T^TAA
MunI 1441 CAATTG C^AATTG
NcoI 3593 CCATGG C^CATGG
NruI 344 TCGCGA TCG^CGA
PaeR7I 3697 CTCGAG C^TCGAG
PinAI 110 ACCGGT A^CCGGT
RcaI 1300 TCATGA T^CATGA
SalI 2759 GTCGAC G^TCGAC
SbfI 2980 CCTGCAGG CCTGCA^GG
SexAI 871 ACCTGGT A^CC[AT]GGT
Sse8387I 2980 CCTGCAGG CCTGCA^GG
Tru9I 2809 TTAA T^TAA
XhoI 3697 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2249 GTCTAC GT^[AC][GT]AC
2759 GTCGAC
AcsI 1044 GAATTC [GA]^AATT[CT]
1257 GAATTT
AhdI 1521 GACACCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2689 GACCAAGCGTC
AlwI 3013 GGATC GGATC(4/5)
3380 GGATC
AlwNI 711 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
2952 CAGCCTCTG
AocI 1082 CCTGAGG CC^T[ACGT]AGG
3529 CCTTAGG
ApaI 2355 GGGCCC GGGCC^C
3203 GGGCCC
ApoI 1044 GAATTC [GA]^AATT[CT]
1257 GAATTT
AspEI 1521 GACACCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2689 GACCAAGCGTC
BalI 1489 TGGCCA TGG^CCA
2272 TGGCCA
BbrPI 681 CACGTG CAC^GTG
849 CACGTG
BfaI 4 CTAG C^TAG
1455 CTAG
BglII 2059 AGATCT A^GATCT
3737 AGATCT
BsmI 1174 GAATGC GAATGC(1/-1)
1746 GAATGC
Bsu36I 1082 CCTGAGG CC^T[ACGT]AGG
3529 CCTTAGG
DraIII 1062 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3519 CACCCTGTG
Eam1105I 1521 GACACCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2689 GACCAAGCGTC
EarI 3438 CTCTTC CTCTTC(1/4)
1244 GAAGAG
Eco81I 1082 CCTGAGG CC^T[ACGT]AGG
3529 CCTTAGG
EcoT22I 1176 ATGCAT ATGCA^T
1748 ATGCAT
HincII 1283 GTTGAC GT[CT]^[GA]AC
2759 GTCGAC
HindII 1283 GTTGAC GT[CT]^[GA]AC
2759 GTCGAC
Ksp632I 3438 CTCTTC CTCTTC(1/4)
1244 GAAGAG
MaeI 4 CTAG C^TAG
1455 CTAG
MluNI 1489 TGGCCA TGG^CCA
2272 TGGCCA
MscI 1489 TGGCCA TGG^CCA
2272 TGGCCA
NsiI 1176 ATGCAT ATGCA^T
1748 ATGCAT
NspI 901 ACATGC [GA]CATG^[CT]
2701 GCATGT
PflMI 740 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3593 CCATGGAATGG
PmaCI 681 CACGTG CAC^GTG
849 CACGTG
PmlI 681 CACGTG CAC^GTG
849 CACGTG
SgrAI 168 CGCCGGCG C[GA]^CCGG[CT]G
2105 CGCCGGCG
SmaI 52 CCCGGG CCC^GGG
294 CCCGGG
Van91I 740 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3593 CCATGGAATGG
XcmI 1477 CCAAGGGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3190 CCACTTGAGTTGTGG
XmaCI 52 CCCGGG C^CCGGG
294 CCCGGG
XmaI 52 CCCGGG C^CCGGG
294 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcnI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII