##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AclI AcsI AgeI ApoI
AscI AseI AsnI Asp700I AspI
AvrII BbrPI BbsI BciVI BlnI
BpuAI BsaBI BsiWI BsmI BspDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI MamI
MfeI MluI MunI NcoI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SalI ScaI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tsp509I Tth111I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1506 GACGTC GACGT^C
Acc65I 1129 GGTACC G^GTACC
AccIII 2666 TCCGGA T^CCGGA
AflII 1866 CTTAAG C^TTAAG
AflIII 799 ACATGT A^C[GA][CT]GT
AhdI 2205 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2016 GTGCAC G^TGCAC
AocI 2556 CCTCAGG CC^T[ACGT]AGG
ApaLI 2016 GTGCAC G^TGCAC
Asp718I 1129 GGTACC G^GTACC
AspEI 2205 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2239 TGGCCA TGG^CCA
BamHI 3552 GGATCC G^GATCC
BbvCI 2909 GCTGAGG CCTCAGC(-5/-2)
BclI 2047 TGATCA T^GATCA
BfrI 1866 CTTAAG C^TTAAG
BglII 1870 AGATCT A^GATCT
BsaAI 1685 CACGTA [CT]AC^GT[GA]
BseAI 2666 TCCGGA T^CCGGA
Bsp1407I 2305 TGTACA T^GTACA
BspEI 2666 TCCGGA T^CCGGA
BspHI 544 TCATGA T^CATGA
BspLU11I 799 ACATGT A^CATGT
BsrDI 920 CATTGC GCAATG(2/0)
BsrGI 2305 TGTACA T^GTACA
Bsu36I 2556 CCTCAGG CC^T[ACGT]AGG
Eam1105I 2205 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2556 CCTCAGG CC^T[ACGT]AGG
FbaI 2047 TGATCA T^GATCA
KpnI 1129 GGTACC GGTAC^C
MluNI 2239 TGGCCA TGG^CCA
MroI 2666 TCCGGA T^CCGGA
MscI 2239 TGGCCA TGG^CCA
NheI 1848 GCTAGC G^CTAGC
PshAI 3323 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1712 CGATCG CGAT^CG
RcaI 544 TCATGA T^CATGA
SexAI 1132 ACCTGGT A^CC[AT]GGT
SspBI 2305 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AviII 10 TGCGCA TGC^GCA
1729 TGCGCA
BbeI 758 GGCGCC GGCGC^C
1592 GGCGCC
BcgI 2636 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2651 CGAGGAGCCTGC
BspMI 202 ACCTGC ACCTGC(4/8)
1765 GCAGGT
BsrFI 367 GCCGGC [GA]^CCGG[CT]
2869 GCCGGC
BssSI 1721 CACGAG CACGAG(-5/-1)
2262 CACGAG
Cfr10I 367 GCCGGC [GA]^CCGG[CT]
2869 GCCGGC
DraIII 1207 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2010 CACATCGTG
EagI 2341 CGGCCG C^GGCCG
2647 CGGCCG
EclXI 2341 CGGCCG C^GGCCG
2647 CGGCCG
Eco52I 2341 CGGCCG C^GGCCG
2647 CGGCCG
EcoNI 380 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1556 CCTGATCCAGG
FspI 10 TGCGCA TGC^GCA
1729 TGCGCA
KasI 758 GGCGCC G^GCGCC
1592 GGCGCC
KspI 49 CCGCGG CCGC^GG
1597 CCGCGG
MseI 1867 TTAA T^TAA
3769 TTAA
NaeI 367 GCCGGC GCC^GGC
2869 GCCGGC
NarI 758 GGCGCC GG^CGCC
1592 GGCGCC
NdeI 1124 CATATG CA^TATG
1436 CATATG
NgoMIV 367 GCCGGC G^CCGGC
2869 GCCGGC
PaeR7I 1335 CTCGAG C^TCGAG
3472 CTCGAG
SacI 954 GAGCTC GAGCT^C
2265 GAGCTC
SacII 49 CCGCGG CCGC^GG
1597 CCGCGG
SapI 2823 GAAGAGC GCTCTTC(1/4)
1797 GCTCTTC
SbfI 203 CCTGCAGG CCTGCA^GG
1979 CCTGCAGG
SfiI 2717 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3603 GGCCAGACAGGCC
SfoI 758 GGCGCC GGC^GCC
1592 GGCGCC
Sse8387I 203 CCTGCAGG CCTGCA^GG
1979 CCTGCAGG
TaqI 1336 TCGA T^CGA
3473 TCGA
Tru9I 1867 TTAA T^TAA
3769 TTAA
TthHB8I 1336 TCGA T^CGA
3473 TCGA
XhoI 1335 CTCGAG C^TCGAG
3472 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI TseI Tsp45I
TspRI Van91I XcmI XhoII