################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccI AclI AcsI AgeI ApoI AscI AseI AsnI Asp700I AspI AvrII BbrPI BbsI BciVI BlnI BpuAI BsaBI BsiWI BsmI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI MamI MfeI MluI MunI NcoI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SalI ScaI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tsp509I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 1506 GACGTC GACGT^C Acc65I 1129 GGTACC G^GTACC AccIII 2666 TCCGGA T^CCGGA AflII 1866 CTTAAG C^TTAAG AflIII 799 ACATGT A^C[GA][CT]GT AhdI 2205 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2016 GTGCAC G^TGCAC AocI 2556 CCTCAGG CC^T[ACGT]AGG ApaLI 2016 GTGCAC G^TGCAC Asp718I 1129 GGTACC G^GTACC AspEI 2205 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2239 TGGCCA TGG^CCA BamHI 3552 GGATCC G^GATCC BbvCI 2909 GCTGAGG CCTCAGC(-5/-2) BclI 2047 TGATCA T^GATCA BfrI 1866 CTTAAG C^TTAAG BglII 1870 AGATCT A^GATCT BsaAI 1685 CACGTA [CT]AC^GT[GA] BseAI 2666 TCCGGA T^CCGGA Bsp1407I 2305 TGTACA T^GTACA BspEI 2666 TCCGGA T^CCGGA BspHI 544 TCATGA T^CATGA BspLU11I 799 ACATGT A^CATGT BsrDI 920 CATTGC GCAATG(2/0) BsrGI 2305 TGTACA T^GTACA Bsu36I 2556 CCTCAGG CC^T[ACGT]AGG Eam1105I 2205 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2556 CCTCAGG CC^T[ACGT]AGG FbaI 2047 TGATCA T^GATCA KpnI 1129 GGTACC GGTAC^C MluNI 2239 TGGCCA TGG^CCA MroI 2666 TCCGGA T^CCGGA MscI 2239 TGGCCA TGG^CCA NheI 1848 GCTAGC G^CTAGC PshAI 3323 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1712 CGATCG CGAT^CG RcaI 544 TCATGA T^CATGA SexAI 1132 ACCTGGT A^CC[AT]GGT SspBI 2305 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AviII 10 TGCGCA TGC^GCA 1729 TGCGCA BbeI 758 GGCGCC GGCGC^C 1592 GGCGCC BcgI 2636 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2651 CGAGGAGCCTGC BspMI 202 ACCTGC ACCTGC(4/8) 1765 GCAGGT BsrFI 367 GCCGGC [GA]^CCGG[CT] 2869 GCCGGC BssSI 1721 CACGAG CACGAG(-5/-1) 2262 CACGAG Cfr10I 367 GCCGGC [GA]^CCGG[CT] 2869 GCCGGC DraIII 1207 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 2010 CACATCGTG EagI 2341 CGGCCG C^GGCCG 2647 CGGCCG EclXI 2341 CGGCCG C^GGCCG 2647 CGGCCG Eco52I 2341 CGGCCG C^GGCCG 2647 CGGCCG EcoNI 380 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1556 CCTGATCCAGG FspI 10 TGCGCA TGC^GCA 1729 TGCGCA KasI 758 GGCGCC G^GCGCC 1592 GGCGCC KspI 49 CCGCGG CCGC^GG 1597 CCGCGG MseI 1867 TTAA T^TAA 3769 TTAA NaeI 367 GCCGGC GCC^GGC 2869 GCCGGC NarI 758 GGCGCC GG^CGCC 1592 GGCGCC NdeI 1124 CATATG CA^TATG 1436 CATATG NgoMIV 367 GCCGGC G^CCGGC 2869 GCCGGC PaeR7I 1335 CTCGAG C^TCGAG 3472 CTCGAG SacI 954 GAGCTC GAGCT^C 2265 GAGCTC SacII 49 CCGCGG CCGC^GG 1597 CCGCGG SapI 2823 GAAGAGC GCTCTTC(1/4) 1797 GCTCTTC SbfI 203 CCTGCAGG CCTGCA^GG 1979 CCTGCAGG SfiI 2717 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3603 GGCCAGACAGGCC SfoI 758 GGCGCC GGC^GCC 1592 GGCGCC Sse8387I 203 CCTGCAGG CCTGCA^GG 1979 CCTGCAGG TaqI 1336 TCGA T^CGA 3473 TCGA Tru9I 1867 TTAA T^TAA 3769 TTAA TthHB8I 1336 TCGA T^CGA 3473 TCGA XhoI 1335 CTCGAG C^TCGAG 3472 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI TseI Tsp45I TspRI Van91I XcmI XhoII