##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AcsI AgeI ApoI AscI
AseI AsnI AspI AvrII BbrPI
BbsI BciVI BlnI BpuAI BsaBI
BsiWI BsmI BspDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DrdI EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
MamI MfeI MluI MunI NcoI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I SalI ScaI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tsp509I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1606 GGTACC G^GTACC
AccI 333 GTCTAC GT^[AC][GT]AC
AflII 2343 CTTAAG C^TTAAG
AflIII 1276 ACATGT A^C[GA][CT]GT
AhdI 2682 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2493 GTGCAC G^TGCAC
AocI 3033 CCTCAGG CC^T[ACGT]AGG
ApaLI 2493 GTGCAC G^TGCAC
Asp700I 93 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1606 GGTACC G^GTACC
AspEI 2682 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2716 TGGCCA TGG^CCA
BbvCI 3386 GCTGAGG CCTCAGC(-5/-2)
BclI 2524 TGATCA T^GATCA
BfrI 2343 CTTAAG C^TTAAG
BglII 2347 AGATCT A^GATCT
Bsp1407I 2782 TGTACA T^GTACA
BspHI 1021 TCATGA T^CATGA
BspLU11I 1276 ACATGT A^CATGT
BsrDI 1397 CATTGC GCAATG(2/0)
BsrGI 2782 TGTACA T^GTACA
Bsu36I 3033 CCTCAGG CC^T[ACGT]AGG
CpoI 210 CGGTCCG CG^G[AT]CCG
Eam1105I 2682 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3033 CCTCAGG CC^T[ACGT]AGG
FbaI 2524 TGATCA T^GATCA
KpnI 1606 GGTACC GGTAC^C
MluNI 2716 TGGCCA TGG^CCA
MscI 2716 TGGCCA TGG^CCA
NheI 2325 GCTAGC G^CTAGC
NotI 446 GCGGCCGC GC^GGCCGC
PshAI 3800 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2189 CGATCG CGAT^CG
RcaI 1021 TCATGA T^CATGA
RsrII 210 CGGTCCG CG^G[AT]CCG
SexAI 1609 ACCTGGT A^CC[AT]GGT
SspBI 2782 TGTACA T^GTACA
XmnI 93 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 40 GACGTC GACGT^C
1983 GACGTC
AccIII 55 TCCGGA T^CCGGA
3143 TCCGGA
AviII 463 TGCGCA TGC^GCA
2206 TGCGCA
BamHI 58 GGATCC G^GATCC
4029 GGATCC
BcgI 3113 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3128 CGAGGAGCCTGC
BsaAI 336 TACGTG [CT]AC^GT[GA]
2162 CACGTA
BseAI 55 TCCGGA T^CCGGA
3143 TCCGGA
BspEI 55 TCCGGA T^CCGGA
3143 TCCGGA
BssSI 2198 CACGAG CACGAG(-5/-1)
2739 CACGAG
DraIII 1684 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2487 CACATCGTG
FspI 463 TGCGCA TGC^GCA
2206 TGCGCA
MroI 55 TCCGGA T^CCGGA
3143 TCCGGA
MseI 2344 TTAA T^TAA
4246 TTAA
NdeI 1601 CATATG CA^TATG
1913 CATATG
SacI 1431 GAGCTC GAGCT^C
2742 GAGCTC
SapI 3300 GAAGAGC GCTCTTC(1/4)
2274 GCTCTTC
SbfI 656 CCTGCAGG CCTGCA^GG
2456 CCTGCAGG
SfiI 3194 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4080 GGCCAGACAGGCC
Sse8387I 656 CCTGCAGG CCTGCA^GG
2456 CCTGCAGG
Tru9I 2344 TTAA T^TAA
4246 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI