##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbeI BbsI BciVI BclI BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspEI BspLU11I BsrGI
Bst1107I BstBI BstZ17I CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI HgaI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 2311 CTTAAG C^TTAAG
AocI 1606 CCTCAGG CC^T[ACGT]AGG
AviII 752 TGCGCA TGC^GCA
AvrII 2305 CCTAGG C^CTAGG
BbrPI 841 CACGTG CAC^GTG
BfrI 2311 CTTAAG C^TTAAG
BglII 2410 AGATCT A^GATCT
BlnI 2305 CCTAGG C^CTAGG
BsaAI 841 CACGTG [CT]AC^GT[GA]
BspDI 3392 ATCGAT AT^CGAT
BspHI 2924 TCATGA T^CATGA
BsrBI 281 GAGCGG CCGCTC(-3/-3)
BsrDI 2062 GCAATG GCAATG(2/0)
BsrFI 255 GCCGGT [GA]^CCGG[CT]
BstEII 379 GGTGACC G^GT[ACGT]ACC
BstPI 379 GGTGACC G^GT[ACGT]ACC
Bsu36I 1606 CCTCAGG CC^T[ACGT]AGG
Cfr10I 255 GCCGGT [GA]^CCGG[CT]
ClaI 3392 ATCGAT AT^CGAT
DraIII 841 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1209 GACTCGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1606 CCTCAGG CC^T[ACGT]AGG
EcoO65I 379 GGTGACC G^GT[ACGT]ACC
FspI 752 TGCGCA TGC^GCA
HaeII 3764 GGCGCT [GA]GCGC^[CT]
HincII 3276 GTCAAC GT[CT]^[GA]AC
HindII 3276 GTCAAC GT[CT]^[GA]AC
NheI 2155 GCTAGC G^CTAGC
PmaCI 841 CACGTG CAC^GTG
PmlI 841 CACGTG CAC^GTG
PshAI 3083 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2924 TCATGA T^CATGA
SacI 3728 GAGCTC GAGCT^C
SbfI 2828 CCTGCAGG CCTGCA^GG
SphI 2363 GCATGC GCATG^C
Sse8387I 2828 CCTGCAGG CCTGCA^GG
XbaI 4509 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AatII 700 GACGTC GACGT^C
769 GACGTC
AcyI 700 GACGTC G[GA]^CG[CT]C
769 GACGTC
BalI 1013 TGGCCA TGG^CCA
4352 TGGCCA
BcgI 1480 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1776 CGATTCTCCTGC
BlpI 626 GCTGAGC GC^T[ACGT]AGC
3602 GCTGAGC
Bpu1102I 626 GCTGAGC GC^T[ACGT]AGC
3602 GCTGAGC
BsaHI 700 GACGTC G[GA]^CG[CT]C
769 GACGTC
BssSI 883 CACGAG CACGAG(-5/-1)
2997 CTCGTG
BstAPI 1445 GCAATCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3805 GCACGTCTTGC
CelII 626 GCTGAGC GC^T[ACGT]AGC
3602 GCTGAGC
Hin1I 700 GACGTC G[GA]^CG[CT]C
769 GACGTC
MluNI 1013 TGGCCA TGG^CCA
4352 TGGCCA
MscI 1013 TGGCCA TGG^CCA
4352 TGGCCA
NcoI 2571 CCATGG C^CATGG
2638 CCATGG
NspI 2363 GCATGC [GA]CATG^[CT]
4292 ACATGC
SspI 4312 AATATT AAT^ATT
4624 AATATT
StuI 1047 AGGCCT AGG^CCT
2959 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PaeR7I
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoI XhoII XmaCI XmaI