################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccIII AclI AgeI Aor51HI AscI AseI AsnI BamHI BlpI Bpu1102I BseAI BsiWI BsmBI BspDI BspEI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI Eco47III EcoO65I EcoRV EcoT22I FseI HindIII HpaI MfeI MluI MroI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 510 GGTACC G^GTACC AcsI 609 GAATTC [GA]^AATT[CT] AcyI 147 GGCGCC G[GA]^CG[CT]C AflII 1745 CTTAAG C^TTAAG AflIII 4082 ACGTGT A^C[GA][CT]GT AhdI 2018 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1720 GTGCAC G^TGCAC ApaLI 1720 GTGCAC G^TGCAC ApoI 609 GAATTC [GA]^AATT[CT] Asp700I 2248 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 510 GGTACC G^GTACC AspEI 2018 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3461 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1434 TGCGCA TGC^GCA AvrII 49 CCTAGG C^CTAGG BbeI 147 GGCGCC GGCGC^C BciVI 488 GGATAC GTATCC(6/5) BfrI 1745 CTTAAG C^TTAAG BlnI 49 CCTAGG C^CTAGG BsaBI 2445 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 147 GGCGCC G[GA]^CG[CT]C BsaWI 2145 TCCGGT [AT]^CCGG[AT] BsrBI 2036 GAGCGG CCGCTC(-3/-3) BssHII 250 GCGCGC G^CGCGC BstAPI 4144 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 1088 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 881 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2018 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 609 GAATTC G^AATTC FspI 1434 TGCGCA TGC^GCA HgaI 3758 GCGTC GACGC(5/10) Hin1I 147 GGCGCC G[GA]^CG[CT]C HincII 3971 GTCAAC GT[CT]^[GA]AC HindII 3971 GTCAAC GT[CT]^[GA]AC KasI 147 GGCGCC G^GCGCC KpnI 510 GGTACC GGTAC^C MamI 2445 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 147 GGCGCC GG^CGCC NcoI 4173 CCATGG C^CATGG NdeI 3016 CATATG CA^TATG PflFI 3461 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4039 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 3669 GCTCTTC GCTCTTC(1/4) ScaI 1419 AGTACT AGT^ACT SfoI 147 GGCGCC GGC^GCC Tth111I 3461 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 2248 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 2151 GTCTAC GT^[AC][GT]AC 3116 GTCTAC BalI 684 TGGCCA TGG^CCA 2311 TGGCCA BcgI 200 CGACTCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1119 GCATCAGCATCG BclI 450 TGATCA T^GATCA 3728 TGATCA BglII 1278 AGATCT A^GATCT 3752 AGATCT Bsp1407I 978 TGTACA T^GTACA 4185 TGTACA BspHI 1366 TCATGA T^CATGA 1695 TCATGA BsrDI 2197 CATTGC GCAATG(2/0) 3783 CATTGC BsrGI 978 TGTACA T^GTACA 4185 TGTACA BssSI 2236 CACGAG CACGAG(-5/-1) 2783 CACGAG EarI 3670 CTCTTC CTCTTC(1/4) 497 GAAGAG FbaI 450 TGATCA T^GATCA 3728 TGATCA Ksp632I 3670 CTCTTC CTCTTC(1/4) 497 GAAGAG KspI 873 CCGCGG CCGC^GG 3241 CCGCGG MluNI 684 TGGCCA TGG^CCA 2311 TGGCCA MscI 684 TGGCCA TGG^CCA 2311 TGGCCA RcaI 1366 TCATGA T^CATGA 1695 TCATGA SacII 873 CCGCGG CCGC^GG 3241 CCGCGG SexAI 639 ACCTGGT A^CC[AT]GGT 1889 ACCTGGT SmaI 21 CCCGGG CCC^GGG 1962 CCCGGG SspBI 978 TGTACA T^GTACA 4185 TGTACA Tsp509I 308 AATT ^AATT 610 AATT XmaCI 21 CCCGGG C^CCGGG 1962 CCCGGG XmaI 21 CCCGGG C^CCGGG 1962 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XhoII