##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AseI AsnI BamHI BlpI
Bpu1102I BseAI BsiWI BsmBI BspDI
BspEI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI Eco47III EcoO65I EcoRV EcoT22I
FseI HindIII HpaI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 510 GGTACC G^GTACC
AcsI 609 GAATTC [GA]^AATT[CT]
AcyI 147 GGCGCC G[GA]^CG[CT]C
AflII 1745 CTTAAG C^TTAAG
AflIII 4082 ACGTGT A^C[GA][CT]GT
AhdI 2018 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1720 GTGCAC G^TGCAC
ApaLI 1720 GTGCAC G^TGCAC
ApoI 609 GAATTC [GA]^AATT[CT]
Asp700I 2248 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 510 GGTACC G^GTACC
AspEI 2018 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3461 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1434 TGCGCA TGC^GCA
AvrII 49 CCTAGG C^CTAGG
BbeI 147 GGCGCC GGCGC^C
BciVI 488 GGATAC GTATCC(6/5)
BfrI 1745 CTTAAG C^TTAAG
BlnI 49 CCTAGG C^CTAGG
BsaBI 2445 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 147 GGCGCC G[GA]^CG[CT]C
BsaWI 2145 TCCGGT [AT]^CCGG[AT]
BsrBI 2036 GAGCGG CCGCTC(-3/-3)
BssHII 250 GCGCGC G^CGCGC
BstAPI 4144 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1088 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 881 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2018 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 609 GAATTC G^AATTC
FspI 1434 TGCGCA TGC^GCA
HgaI 3758 GCGTC GACGC(5/10)
Hin1I 147 GGCGCC G[GA]^CG[CT]C
HincII 3971 GTCAAC GT[CT]^[GA]AC
HindII 3971 GTCAAC GT[CT]^[GA]AC
KasI 147 GGCGCC G^GCGCC
KpnI 510 GGTACC GGTAC^C
MamI 2445 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 147 GGCGCC GG^CGCC
NcoI 4173 CCATGG C^CATGG
NdeI 3016 CATATG CA^TATG
PflFI 3461 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4039 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3669 GCTCTTC GCTCTTC(1/4)
ScaI 1419 AGTACT AGT^ACT
SfoI 147 GGCGCC GGC^GCC
Tth111I 3461 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2248 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2151 GTCTAC GT^[AC][GT]AC
3116 GTCTAC
BalI 684 TGGCCA TGG^CCA
2311 TGGCCA
BcgI 200 CGACTCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1119 GCATCAGCATCG
BclI 450 TGATCA T^GATCA
3728 TGATCA
BglII 1278 AGATCT A^GATCT
3752 AGATCT
Bsp1407I 978 TGTACA T^GTACA
4185 TGTACA
BspHI 1366 TCATGA T^CATGA
1695 TCATGA
BsrDI 2197 CATTGC GCAATG(2/0)
3783 CATTGC
BsrGI 978 TGTACA T^GTACA
4185 TGTACA
BssSI 2236 CACGAG CACGAG(-5/-1)
2783 CACGAG
EarI 3670 CTCTTC CTCTTC(1/4)
497 GAAGAG
FbaI 450 TGATCA T^GATCA
3728 TGATCA
Ksp632I 3670 CTCTTC CTCTTC(1/4)
497 GAAGAG
KspI 873 CCGCGG CCGC^GG
3241 CCGCGG
MluNI 684 TGGCCA TGG^CCA
2311 TGGCCA
MscI 684 TGGCCA TGG^CCA
2311 TGGCCA
RcaI 1366 TCATGA T^CATGA
1695 TCATGA
SacII 873 CCGCGG CCGC^GG
3241 CCGCGG
SexAI 639 ACCTGGT A^CC[AT]GGT
1889 ACCTGGT
SmaI 21 CCCGGG CCC^GGG
1962 CCCGGG
SspBI 978 TGTACA T^GTACA
4185 TGTACA
Tsp509I 308 AATT ^AATT
610 AATT
XmaCI 21 CCCGGG C^CCGGG
1962 CCCGGG
XmaI 21 CCCGGG C^CCGGG
1962 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII