################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AocI ApaI AscI Asp718I AviII BbrPI BciVI BfrI BglI BglII BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BspEI BspHI BsrFI BssHII BstBI BstEII BstPI Bsu36I CelII Cfr10I CpoI EagI EclXI Eco52I Eco81I EcoO65I EcoT22I FseI FspI HindIII KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 52 GACGTC GACGT^C AccI 3480 GTATAC GT^[AC][GT]AC AflIII 2709 ACATGT A^C[GA][CT]GT AhdI 2583 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4587 GTGCAC G^TGCAC AlwNI 4543 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 4587 GTGCAC G^TGCAC AseI 2703 ATTAAT AT^TAAT AsnI 2703 ATTAAT AT^TAAT Asp700I 2874 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2583 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2542 CCTAGG C^CTAGG BalI 4295 TGGCCA TGG^CCA BamHI 1285 GGATCC G^GATCC BcgI 4046 GCAGGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2291 TGATCA T^GATCA BlnI 2542 CCTAGG C^CTAGG BsaAI 1006 CACGTA [CT]AC^GT[GA] BsmBI 1515 GAGACG CGTCTC(1/5) BspDI 641 ATCGAT AT^CGAT BspLU11I 2709 ACATGT A^CATGT BsrBI 4566 CCGCTC CCGCTC(-3/-3) Bst1107I 3480 GTATAC GTA^TAC BstXI 1452 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3480 GTATAC GTA^TAC ClaI 641 ATCGAT AT^CGAT DraIII 626 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 52 GACGTCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2583 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2529 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1163 GATATC GAT^ATC FbaI 2291 TGATCA T^GATCA HpaI 2401 GTTAAC GTT^AAC MluNI 4295 TGGCCA TGG^CCA MscI 4295 TGGCCA TGG^CCA PmeI 949 GTTTAAAC GTTT^AAAC PshBI 2703 ATTAAT AT^TAAT SapI 592 GAAGAGC GCTCTTC(1/4) ScaI 447 AGTACT AGT^ACT SexAI 627 ACCTGGT A^CC[AT]GGT StuI 2907 AGGCCT AGG^CCT XcmI 2587 CCAAGTCCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2874 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 58 CGCG CG^CG 92 CGCG Aor51HI 501 AGCGCT AGC^GCT 4559 AGCGCT AspI 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4164 GACCGTGTC BanII 3034 GAGCTC G[GA]GC[CT]^C 4150 GAGCTC BbeI 83 GGCGCC GGCGC^C 2525 GGCGCC BsaBI 634 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1629 GATGCCCATC BsmI 3905 GAATGC GAATGC(1/-1) 4117 GCATTC Bsp1407I 426 TGTACA T^GTACA 540 TGTACA BsrGI 426 TGTACA T^GTACA 540 TGTACA BssSI 794 CACGAG CACGAG(-5/-1) 3419 CACGAG BstAPI 1106 GCAAATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4364 GCAAAAATTGC BstUI 58 CGCG CG^CG 92 CGCG EaeI 1251 TGGCCG [CT]^GGCC[GA] 4295 TGGCCA EarI 592 GAAGAG CTCTTC(1/4) 3337 GAAGAG Eco47III 501 AGCGCT AGC^GCT 4559 AGCGCT EcoRI 102 GAATTC G^AATTC 1649 GAATTC HincII 2081 GTCAAC GT[CT]^[GA]AC 2401 GTTAAC HindII 2081 GTCAAC GT[CT]^[GA]AC 2401 GTTAAC KasI 83 GGCGCC G^GCGCC 2525 GGCGCC Ksp632I 592 GAAGAG CTCTTC(1/4) 3337 GAAGAG MaeII 53 ACGT A^CGT 1007 ACGT MamI 634 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1629 GATGCCCATC MvnI 58 CGCG CG^CG 92 CGCG NarI 83 GGCGCC GG^CGCC 2525 GGCGCC NdeI 1843 CATATG CA^TATG 3039 CATATG NspI 2357 GCATGT [GA]CATG^[CT] 2709 ACATGT PflFI 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4164 GACCGTGTC PflMI 1888 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3384 CCAATTTTTGG PpuMI 3020 AGGTCCT [GA]G^G[AT]CC[CT] 3580 GGGACCC PvuII 944 CAGCTG CAG^CTG 4278 CAGCTG SacI 3034 GAGCTC GAGCT^C 4150 GAGCTC SfoI 83 GGCGCC GGC^GCC 2525 GGCGCC SmaI 66 CCCGGG CCC^GGG 1419 CCCGGG SspBI 426 TGTACA T^GTACA 540 TGTACA SspI 2721 AATATT AAT^ATT 3961 AATATT Tth111I 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4164 GACCGTGTC Van91I 1888 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3384 CCAATTTTTGG XmaCI 66 CCCGGG C^CCGGG 1419 CCCGGG XmaI 66 CCCGGG C^CCGGG 1419 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII