##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AocI ApaI AscI Asp718I AviII
BbrPI BciVI BfrI BglI BglII
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BspEI BspHI BsrFI BssHII
BstBI BstEII BstPI Bsu36I CelII
Cfr10I CpoI EagI EclXI Eco52I
Eco81I EcoO65I EcoT22I FseI FspI
HindIII KpnI KspI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 52 GACGTC GACGT^C
AccI 3480 GTATAC GT^[AC][GT]AC
AflIII 2709 ACATGT A^C[GA][CT]GT
AhdI 2583 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4587 GTGCAC G^TGCAC
AlwNI 4543 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 4587 GTGCAC G^TGCAC
AseI 2703 ATTAAT AT^TAAT
AsnI 2703 ATTAAT AT^TAAT
Asp700I 2874 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2583 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2542 CCTAGG C^CTAGG
BalI 4295 TGGCCA TGG^CCA
BamHI 1285 GGATCC G^GATCC
BcgI 4046 GCAGGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2291 TGATCA T^GATCA
BlnI 2542 CCTAGG C^CTAGG
BsaAI 1006 CACGTA [CT]AC^GT[GA]
BsmBI 1515 GAGACG CGTCTC(1/5)
BspDI 641 ATCGAT AT^CGAT
BspLU11I 2709 ACATGT A^CATGT
BsrBI 4566 CCGCTC CCGCTC(-3/-3)
Bst1107I 3480 GTATAC GTA^TAC
BstXI 1452 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3480 GTATAC GTA^TAC
ClaI 641 ATCGAT AT^CGAT
DraIII 626 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 52 GACGTCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2583 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2529 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1163 GATATC GAT^ATC
FbaI 2291 TGATCA T^GATCA
HpaI 2401 GTTAAC GTT^AAC
MluNI 4295 TGGCCA TGG^CCA
MscI 4295 TGGCCA TGG^CCA
PmeI 949 GTTTAAAC GTTT^AAAC
PshBI 2703 ATTAAT AT^TAAT
SapI 592 GAAGAGC GCTCTTC(1/4)
ScaI 447 AGTACT AGT^ACT
SexAI 627 ACCTGGT A^CC[AT]GGT
StuI 2907 AGGCCT AGG^CCT
XcmI 2587 CCAAGTCCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2874 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 58 CGCG CG^CG
92 CGCG
Aor51HI 501 AGCGCT AGC^GCT
4559 AGCGCT
AspI 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4164 GACCGTGTC
BanII 3034 GAGCTC G[GA]GC[CT]^C
4150 GAGCTC
BbeI 83 GGCGCC GGCGC^C
2525 GGCGCC
BsaBI 634 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1629 GATGCCCATC
BsmI 3905 GAATGC GAATGC(1/-1)
4117 GCATTC
Bsp1407I 426 TGTACA T^GTACA
540 TGTACA
BsrGI 426 TGTACA T^GTACA
540 TGTACA
BssSI 794 CACGAG CACGAG(-5/-1)
3419 CACGAG
BstAPI 1106 GCAAATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4364 GCAAAAATTGC
BstUI 58 CGCG CG^CG
92 CGCG
EaeI 1251 TGGCCG [CT]^GGCC[GA]
4295 TGGCCA
EarI 592 GAAGAG CTCTTC(1/4)
3337 GAAGAG
Eco47III 501 AGCGCT AGC^GCT
4559 AGCGCT
EcoRI 102 GAATTC G^AATTC
1649 GAATTC
HincII 2081 GTCAAC GT[CT]^[GA]AC
2401 GTTAAC
HindII 2081 GTCAAC GT[CT]^[GA]AC
2401 GTTAAC
KasI 83 GGCGCC G^GCGCC
2525 GGCGCC
Ksp632I 592 GAAGAG CTCTTC(1/4)
3337 GAAGAG
MaeII 53 ACGT A^CGT
1007 ACGT
MamI 634 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1629 GATGCCCATC
MvnI 58 CGCG CG^CG
92 CGCG
NarI 83 GGCGCC GG^CGCC
2525 GGCGCC
NdeI 1843 CATATG CA^TATG
3039 CATATG
NspI 2357 GCATGT [GA]CATG^[CT]
2709 ACATGT
PflFI 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4164 GACCGTGTC
PflMI 1888 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3384 CCAATTTTTGG
PpuMI 3020 AGGTCCT [GA]G^G[AT]CC[CT]
3580 GGGACCC
PvuII 944 CAGCTG CAG^CTG
4278 CAGCTG
SacI 3034 GAGCTC GAGCT^C
4150 GAGCTC
SfoI 83 GGCGCC GGC^GCC
2525 GGCGCC
SmaI 66 CCCGGG CCC^GGG
1419 CCCGGG
SspBI 426 TGTACA T^GTACA
540 TGTACA
SspI 2721 AATATT AAT^ATT
3961 AATATT
Tth111I 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4164 GACCGTGTC
Van91I 1888 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3384 CCAATTTTTGG
XmaCI 66 CCCGGG C^CCGGG
1419 CCCGGG
XmaI 66 CCCGGG C^CCGGG
1419 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII