################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Asp718I AspEI AviII AvrII BamHI BcgI BciVI BfrI BglII BlnI BsaBI BsgI BsiWI BspHI BspMI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DrdI Eam1105I EcoO65I FseI FspI KpnI MamI MluI NheI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 2046 GTCTAC GT^[AC][GT]AC AccIII 1030 TCCGGA T^CCGGA AcyI 221 GGCGCC G[GA]^CG[CT]C Aor51HI 1089 AGCGCT AGC^GCT AscI 114 GGCGCGCC GG^CGCGCC AseI 815 ATTAAT AT^TAAT AsnI 815 ATTAAT AT^TAAT AspI 3286 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 221 GGCGCC GGCGC^C BbrPI 1892 CACGTG CAC^GTG BglI 2012 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2847 GCTCAGC GC^T[ACGT]AGC BpmI 4025 CTGGAG CTGGAG(16/14) Bpu1102I 2847 GCTCAGC GC^T[ACGT]AGC BsaAI 1892 CACGTG [CT]AC^GT[GA] BsaHI 221 GGCGCC G[GA]^CG[CT]C BsaWI 1030 TCCGGA [AT]^CCGG[AT] BseAI 1030 TCCGGA T^CCGGA BsmBI 2074 CGTCTC CGTCTC(1/5) Bsp1407I 2634 TGTACA T^GTACA BspDI 1328 ATCGAT AT^CGAT BspEI 1030 TCCGGA T^CCGGA BspLU11I 280 ACATGT A^CATGT BsrGI 2634 TGTACA T^GTACA BstYI 1263 AGATCC [GA]^GATC[CT] CelII 2847 GCTCAGC GC^T[ACGT]AGC ClaI 1328 ATCGAT AT^CGAT DraIII 2344 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1089 AGCGCT AGC^GCT EcoNI 4049 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3482 GAATTC G^AATTC EcoRV 1819 GATATC GAT^ATC HgaI 1363 GACGC GACGC(5/10) Hin1I 221 GGCGCC G[GA]^CG[CT]C HincII 2617 GTTAAC GT[CT]^[GA]AC HindII 2617 GTTAAC GT[CT]^[GA]AC HpaI 2617 GTTAAC GTT^AAC KasI 221 GGCGCC G^GCGCC KspI 213 CCGCGG CCGC^GG MfeI 3322 CAATTG C^AATTG MflI 1263 AGATCC [GA]^GATC[CT] MroI 1030 TCCGGA T^CCGGA MunI 3322 CAATTG C^AATTG NarI 221 GGCGCC GG^CGCC NotI 1 GCGGCCGC GC^GGCCGC PflFI 3286 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1892 CACGTG CAC^GTG PmlI 1892 CACGTG CAC^GTG PshBI 815 ATTAAT AT^TAAT PvuI 1326 CGATCG CGAT^CG SacII 213 CCGCGG CCGC^GG SfoI 221 GGCGCC GGC^GCC SmaI 178 CCCGGG CCC^GGG SpeI 950 ACTAGT A^CTAGT SspBI 2634 TGTACA T^GTACA Tth111I 3286 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1263 AGATCC [GA]^GATC[CT] XmaCI 178 CCCGGG C^CCGGG XmaI 178 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Alw44I 2932 GTGCAC G^TGCAC 4039 GTGCAC ApaI 169 GGGCCC GGGCC^C 4032 GGGCCC ApaLI 2932 GTGCAC G^TGCAC 4039 GTGCAC Asp700I 803 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4154 GAACTGTTTC AvaI 178 CCCGGG C^[CT]CG[GA]G 207 CTCGGG BalI 2061 TGGCCA TGG^CCA 2520 TGGCCA BbsI 2562 GAAGAC GAAGAC(2/6) 2889 GTCTTC BbvCI 2510 CCTCAGC CCTCAGC(-5/-2) 2902 GCTGAGG BclI 603 TGATCA T^GATCA 4325 TGATCA BpuAI 2562 GAAGAC GAAGAC(2/6) 2889 GTCTTC BsaI 254 GAGACC GGTCTC(1/5) 1969 GAGACC BsoBI 178 CCCGGG C^[CT]CG[GA]G 207 CTCGGG BsrDI 1454 CATTGC GCAATG(2/0) 2489 CATTGC BssHII 115 GCGCGC G^CGCGC 2068 GCGCGC BstAPI 779 GCACTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3412 GCAGGCCATGC BstXI 2257 CCATTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2494 CCAGCCCCGTGG DraI 4107 TTTAAA TTT^AAA 4256 TTTAAA EagI 2 CGGCCG C^GGCCG 65 CGGCCG EclXI 2 CGGCCG C^GGCCG 65 CGGCCG Eco52I 2 CGGCCG C^GGCCG 65 CGGCCG EcoT22I 753 ATGCAT ATGCA^T 2486 ATGCAT FbaI 603 TGATCA T^GATCA 4325 TGATCA MluNI 2061 TGGCCA TGG^CCA 2520 TGGCCA MscI 2061 TGGCCA TGG^CCA 2520 TGGCCA NdeI 3781 CATATG CA^TATG 4329 CATATG NsiI 753 ATGCAT ATGCA^T 2486 ATGCAT PflMI 812 CCAATTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3028 CCAGCTGCTGG SacI 3277 GAGCTC GAGCT^C 3356 GAGCTC ScaI 2405 AGTACT AGT^ACT 2550 AGTACT SmlI 1997 CTCAAG C^T[CT][GA]AG 3338 CTTGAG Van91I 812 CCAATTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3028 CCAGCTGCTGG XmnI 803 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4154 GAACTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI