##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Asp718I AspEI AviII AvrII
BamHI BcgI BciVI BfrI BglII
BlnI BsaBI BsgI BsiWI BspHI
BspMI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DrdI Eam1105I
EcoO65I FseI FspI KpnI MamI
MluI NheI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 2046 GTCTAC GT^[AC][GT]AC
AccIII 1030 TCCGGA T^CCGGA
AcyI 221 GGCGCC G[GA]^CG[CT]C
Aor51HI 1089 AGCGCT AGC^GCT
AscI 114 GGCGCGCC GG^CGCGCC
AseI 815 ATTAAT AT^TAAT
AsnI 815 ATTAAT AT^TAAT
AspI 3286 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 221 GGCGCC GGCGC^C
BbrPI 1892 CACGTG CAC^GTG
BglI 2012 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2847 GCTCAGC GC^T[ACGT]AGC
BpmI 4025 CTGGAG CTGGAG(16/14)
Bpu1102I 2847 GCTCAGC GC^T[ACGT]AGC
BsaAI 1892 CACGTG [CT]AC^GT[GA]
BsaHI 221 GGCGCC G[GA]^CG[CT]C
BsaWI 1030 TCCGGA [AT]^CCGG[AT]
BseAI 1030 TCCGGA T^CCGGA
BsmBI 2074 CGTCTC CGTCTC(1/5)
Bsp1407I 2634 TGTACA T^GTACA
BspDI 1328 ATCGAT AT^CGAT
BspEI 1030 TCCGGA T^CCGGA
BspLU11I 280 ACATGT A^CATGT
BsrGI 2634 TGTACA T^GTACA
BstYI 1263 AGATCC [GA]^GATC[CT]
CelII 2847 GCTCAGC GC^T[ACGT]AGC
ClaI 1328 ATCGAT AT^CGAT
DraIII 2344 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1089 AGCGCT AGC^GCT
EcoNI 4049 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3482 GAATTC G^AATTC
EcoRV 1819 GATATC GAT^ATC
HgaI 1363 GACGC GACGC(5/10)
Hin1I 221 GGCGCC G[GA]^CG[CT]C
HincII 2617 GTTAAC GT[CT]^[GA]AC
HindII 2617 GTTAAC GT[CT]^[GA]AC
HpaI 2617 GTTAAC GTT^AAC
KasI 221 GGCGCC G^GCGCC
KspI 213 CCGCGG CCGC^GG
MfeI 3322 CAATTG C^AATTG
MflI 1263 AGATCC [GA]^GATC[CT]
MroI 1030 TCCGGA T^CCGGA
MunI 3322 CAATTG C^AATTG
NarI 221 GGCGCC GG^CGCC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 3286 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1892 CACGTG CAC^GTG
PmlI 1892 CACGTG CAC^GTG
PshBI 815 ATTAAT AT^TAAT
PvuI 1326 CGATCG CGAT^CG
SacII 213 CCGCGG CCGC^GG
SfoI 221 GGCGCC GGC^GCC
SmaI 178 CCCGGG CCC^GGG
SpeI 950 ACTAGT A^CTAGT
SspBI 2634 TGTACA T^GTACA
Tth111I 3286 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1263 AGATCC [GA]^GATC[CT]
XmaCI 178 CCCGGG C^CCGGG
XmaI 178 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 2932 GTGCAC G^TGCAC
4039 GTGCAC
ApaI 169 GGGCCC GGGCC^C
4032 GGGCCC
ApaLI 2932 GTGCAC G^TGCAC
4039 GTGCAC
Asp700I 803 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4154 GAACTGTTTC
AvaI 178 CCCGGG C^[CT]CG[GA]G
207 CTCGGG
BalI 2061 TGGCCA TGG^CCA
2520 TGGCCA
BbsI 2562 GAAGAC GAAGAC(2/6)
2889 GTCTTC
BbvCI 2510 CCTCAGC CCTCAGC(-5/-2)
2902 GCTGAGG
BclI 603 TGATCA T^GATCA
4325 TGATCA
BpuAI 2562 GAAGAC GAAGAC(2/6)
2889 GTCTTC
BsaI 254 GAGACC GGTCTC(1/5)
1969 GAGACC
BsoBI 178 CCCGGG C^[CT]CG[GA]G
207 CTCGGG
BsrDI 1454 CATTGC GCAATG(2/0)
2489 CATTGC
BssHII 115 GCGCGC G^CGCGC
2068 GCGCGC
BstAPI 779 GCACTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3412 GCAGGCCATGC
BstXI 2257 CCATTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2494 CCAGCCCCGTGG
DraI 4107 TTTAAA TTT^AAA
4256 TTTAAA
EagI 2 CGGCCG C^GGCCG
65 CGGCCG
EclXI 2 CGGCCG C^GGCCG
65 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
65 CGGCCG
EcoT22I 753 ATGCAT ATGCA^T
2486 ATGCAT
FbaI 603 TGATCA T^GATCA
4325 TGATCA
MluNI 2061 TGGCCA TGG^CCA
2520 TGGCCA
MscI 2061 TGGCCA TGG^CCA
2520 TGGCCA
NdeI 3781 CATATG CA^TATG
4329 CATATG
NsiI 753 ATGCAT ATGCA^T
2486 ATGCAT
PflMI 812 CCAATTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3028 CCAGCTGCTGG
SacI 3277 GAGCTC GAGCT^C
3356 GAGCTC
ScaI 2405 AGTACT AGT^ACT
2550 AGTACT
SmlI 1997 CTCAAG C^T[CT][GA]AG
3338 CTTGAG
Van91I 812 CCAATTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3028 CCAGCTGCTGG
XmnI 803 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4154 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI