##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BciVI BfrI BglI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
Bsp1407I BspEI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI CelII
Cfr10I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI HindIII
HpaI KpnI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI PvuII RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 3627 GACGTC GACGT^C
AocI 162 CCTCAGG CC^T[ACGT]AGG
AviII 1642 TGCGCA TGC^GCA
BbeI 2787 GGCGCC GGCGC^C
BbrPI 2512 CACGTG CAC^GTG
BglII 1961 AGATCT A^GATCT
BsaAI 2512 CACGTG [CT]AC^GT[GA]
BsmBI 1331 CGTCTC CGTCTC(1/5)
BspDI 2890 ATCGAT AT^CGAT
BspHI 643 TCATGA T^CATGA
BspLU11I 3254 ACATGT A^CATGT
BspMI 3857 GCAGGT ACCTGC(4/8)
Bst1107I 3861 GTATAC GTA^TAC
BstZ17I 3861 GTATAC GTA^TAC
Bsu36I 162 CCTCAGG CC^T[ACGT]AGG
ClaI 2890 ATCGAT AT^CGAT
Eco81I 162 CCTCAGG CC^T[ACGT]AGG
EcoRI 3702 GAATTC G^AATTC
FspI 1642 TGCGCA TGC^GCA
KasI 2787 GGCGCC G^GCGCC
KspI 3671 CCGCGG CCGC^GG
NarI 2787 GGCGCC GG^CGCC
PmaCI 2512 CACGTG CAC^GTG
PmlI 2512 CACGTG CAC^GTG
PshAI 1298 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 643 TCATGA T^CATGA
SacI 99 GAGCTC GAGCT^C
SacII 3671 CCGCGG CCGC^GG
SfoI 2787 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 3411 GTAGAC GT^[AC][GT]AC
3861 GTATAC
AflIII 2513 ACGTGT A^C[GA][CT]GT
3254 ACATGT
AvaI 2935 CTCGGG C^[CT]CG[GA]G
4162 CCCGAG
BamHI 1822 GGATCC G^GATCC
3217 GGATCC
BbvCI 688 CCTCAGC CCTCAGC(-5/-2)
2532 GCTGAGG
BcgI 2133 CGAGCCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2927 GCAGATTACTCG
BclI 2554 TGATCA T^GATCA
4044 TGATCA
BsaI 2109 GAGACC GGTCTC(1/5)
2207 GGTCTC
BsgI 2226 CTGCAC GTGCAG(16/14)
1704 GTGCAG
BsmI 930 GCATTC GAATGC(1/-1)
2821 GCATTC
BsoBI 2935 CTCGGG C^[CT]CG[GA]G
4162 CCCGAG
BsrDI 1127 CATTGC GCAATG(2/0)
1186 CATTGC
BstAPI 693 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2378 GCAGCTCCTGC
DsaI 2308 CCGTGG C^C[GA][CT]GG
3671 CCGCGG
EcoNI 3900 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4158 CCTTCCCGAGG
FbaI 2554 TGATCA T^GATCA
4044 TGATCA
NspI 1942 ACATGC [GA]CATG^[CT]
3254 ACATGT
PleI 680 GACTC GAGTC(4/5)
2643 GACTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaHI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII