##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI Asp718I AviII
AvrII BbrPI BciVI BclI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BsrDI BstBI
ClaI CpoI DrdI Eco47III EcoRV
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 3469 GACTTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2853 GTGCAC G^TGCAC
ApaLI 2853 GTGCAC G^TGCAC
AspEI 3469 GACTTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3071 GGATCC G^GATCC
BlpI 3831 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3831 GCTGAGC GC^T[ACGT]AGC
BsiEI 3130 CGGCCG CG[GA][CT]^CG
BsmBI 2468 GAGACG CGTCTC(1/5)
BsmI 546 GAATGC GAATGC(1/-1)
BspLU11I 1165 ACATGT A^CATGT
BssHII 1772 GCGCGC G^CGCGC
Bst1107I 3676 GTATAC GTA^TAC
BstEII 3851 GGTCACC G^GT[ACGT]ACC
BstPI 3851 GGTCACC G^GT[ACGT]ACC
BstZ17I 3676 GTATAC GTA^TAC
CelII 3831 GCTGAGC GC^T[ACGT]AGC
DraI 4007 TTTAAA TTT^AAA
DraIII 1195 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3130 CGGCCG C^GGCCG
Eam1105I 3469 GACTTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3130 CGGCCG C^GGCCG
Eco52I 3130 CGGCCG C^GGCCG
EcoO65I 3851 GGTCACC G^GT[ACGT]ACC
EcoRI 1435 GAATTC G^AATTC
HincII 837 GTCAAC GT[CT]^[GA]AC
HindII 837 GTCAAC GT[CT]^[GA]AC
NheI 1827 GCTAGC G^CTAGC
SapI 71 GCTCTTC GCTCTTC(1/4)
SmlI 3371 CTCAAG C^T[CT][GA]AG
SphI 1996 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1435 GAATTC [GA]^AATT[CT]
4028 GAATTT
AflIII 1165 ACATGT A^C[GA][CT]GT
3845 ACGTGT
ApoI 1435 GAATTC [GA]^AATT[CT]
4028 GAATTT
AseI 3908 ATTAAT AT^TAAT
3999 ATTAAT
AsnI 3908 ATTAAT AT^TAAT
3999 ATTAAT
AspI 785 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACTGAGTC
BbeI 1619 GGCGCC GGCGC^C
2953 GGCGCC
BbsI 89 GTCTTC GAAGAC(2/6)
589 GTCTTC
BcgI 2845 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3250 GCACCACTGTCG
BpuAI 89 GTCTTC GAAGAC(2/6)
589 GTCTTC
BsaAI 3823 TACGTG [CT]AC^GT[GA]
3844 TACGTG
BspHI 372 TCATGA T^CATGA
800 TCATGA
BstYI 3071 GGATCC [GA]^GATC[CT]
3558 AGATCC
EarI 72 CTCTTC CTCTTC(1/4)
2366 GAAGAG
EcoT22I 548 ATGCAT ATGCA^T
1994 ATGCAT
HaeII 1619 GGCGCC [GA]GCGC^[CT]
2953 GGCGCC
HindIII 1734 AAGCTT A^AGCTT
4435 AAGCTT
KasI 1619 GGCGCC G^GCGCC
2953 GGCGCC
Ksp632I 72 CTCTTC CTCTTC(1/4)
2366 GAAGAG
MflI 3071 GGATCC [GA]^GATC[CT]
3558 AGATCC
NarI 1619 GGCGCC GG^CGCC
2953 GGCGCC
NsiI 548 ATGCAT ATGCA^T
1994 ATGCAT
PflFI 785 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACTGAGTC
PflMI 2944 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3096 CCAGGGGCTGG
PshAI 601 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2418 GACGGATGTC
PshBI 3908 ATTAAT AT^TAAT
3999 ATTAAT
RcaI 372 TCATGA T^CATGA
800 TCATGA
SfoI 1619 GGCGCC GGC^GCC
2953 GGCGCC
SmaI 3163 CCCGGG CCC^GGG
3194 CCCGGG
Tth111I 785 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACTGAGTC
Van91I 2944 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3096 CCAGGGGCTGG
XhoII 3071 GGATCC [GA]^GATC[CT]
3558 AGATCC
XmaCI 3163 CCCGGG C^CCGGG
3194 CCCGGG
XmaI 3163 CCCGGG C^CCGGG
3194 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmnI