################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Aor51HI AscI Asp718I AviII AvrII BbrPI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BspDI BspEI BsrDI BstBI ClaI CpoI DrdI Eco47III EcoRV FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AhdI 3469 GACTTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2853 GTGCAC G^TGCAC ApaLI 2853 GTGCAC G^TGCAC AspEI 3469 GACTTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3071 GGATCC G^GATCC BlpI 3831 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3831 GCTGAGC GC^T[ACGT]AGC BsiEI 3130 CGGCCG CG[GA][CT]^CG BsmBI 2468 GAGACG CGTCTC(1/5) BsmI 546 GAATGC GAATGC(1/-1) BspLU11I 1165 ACATGT A^CATGT BssHII 1772 GCGCGC G^CGCGC Bst1107I 3676 GTATAC GTA^TAC BstEII 3851 GGTCACC G^GT[ACGT]ACC BstPI 3851 GGTCACC G^GT[ACGT]ACC BstZ17I 3676 GTATAC GTA^TAC CelII 3831 GCTGAGC GC^T[ACGT]AGC DraI 4007 TTTAAA TTT^AAA DraIII 1195 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 3130 CGGCCG C^GGCCG Eam1105I 3469 GACTTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3130 CGGCCG C^GGCCG Eco52I 3130 CGGCCG C^GGCCG EcoO65I 3851 GGTCACC G^GT[ACGT]ACC EcoRI 1435 GAATTC G^AATTC HincII 837 GTCAAC GT[CT]^[GA]AC HindII 837 GTCAAC GT[CT]^[GA]AC NheI 1827 GCTAGC G^CTAGC SapI 71 GCTCTTC GCTCTTC(1/4) SmlI 3371 CTCAAG C^T[CT][GA]AG SphI 1996 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 1435 GAATTC [GA]^AATT[CT] 4028 GAATTT AflIII 1165 ACATGT A^C[GA][CT]GT 3845 ACGTGT ApoI 1435 GAATTC [GA]^AATT[CT] 4028 GAATTT AseI 3908 ATTAAT AT^TAAT 3999 ATTAAT AsnI 3908 ATTAAT AT^TAAT 3999 ATTAAT AspI 785 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3860 GACTGAGTC BbeI 1619 GGCGCC GGCGC^C 2953 GGCGCC BbsI 89 GTCTTC GAAGAC(2/6) 589 GTCTTC BcgI 2845 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3250 GCACCACTGTCG BpuAI 89 GTCTTC GAAGAC(2/6) 589 GTCTTC BsaAI 3823 TACGTG [CT]AC^GT[GA] 3844 TACGTG BspHI 372 TCATGA T^CATGA 800 TCATGA BstYI 3071 GGATCC [GA]^GATC[CT] 3558 AGATCC EarI 72 CTCTTC CTCTTC(1/4) 2366 GAAGAG EcoT22I 548 ATGCAT ATGCA^T 1994 ATGCAT HaeII 1619 GGCGCC [GA]GCGC^[CT] 2953 GGCGCC HindIII 1734 AAGCTT A^AGCTT 4435 AAGCTT KasI 1619 GGCGCC G^GCGCC 2953 GGCGCC Ksp632I 72 CTCTTC CTCTTC(1/4) 2366 GAAGAG MflI 3071 GGATCC [GA]^GATC[CT] 3558 AGATCC NarI 1619 GGCGCC GG^CGCC 2953 GGCGCC NsiI 548 ATGCAT ATGCA^T 1994 ATGCAT PflFI 785 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3860 GACTGAGTC PflMI 2944 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3096 CCAGGGGCTGG PshAI 601 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2418 GACGGATGTC PshBI 3908 ATTAAT AT^TAAT 3999 ATTAAT RcaI 372 TCATGA T^CATGA 800 TCATGA SfoI 1619 GGCGCC GGC^GCC 2953 GGCGCC SmaI 3163 CCCGGG CCC^GGG 3194 CCCGGG Tth111I 785 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3860 GACTGAGTC Van91I 2944 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3096 CCAGGGGCTGG XhoII 3071 GGATCC [GA]^GATC[CT] 3558 AGATCC XmaCI 3163 CCCGGG C^CCGGG 3194 CCCGGG XmaI 3163 CCCGGG C^CCGGG 3194 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI