##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI Aor51HI AscI Asp718I
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BcnI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Cfr10I
ClaI CpoI DraII DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRV FauI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AciI 135 GCGG CCGC(-3/-1)
AclI 3071 AACGTT AA^CGTT
AhdI 2385 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3348 GGGCCC GGGCC^C
AseI 4299 ATTAAT AT^TAAT
AsnI 4299 ATTAAT AT^TAAT
AspEI 2385 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 885 CTCGGG C^[CT]CG[GA]G
BbvCI 2355 GCTGAGG CCTCAGC(-5/-2)
BglI 2616 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2374 GCTGAGC GC^T[ACGT]AGC
BpmI 1569 CTCCAG CTGGAG(16/14)
Bpu1102I 2374 GCTGAGC GC^T[ACGT]AGC
BsmFI 1171 GGGAC GGGAC(10/14)
BsoBI 885 CTCGGG C^[CT]CG[GA]G
BstEII 1634 GGTCACC G^GT[ACGT]ACC
BstPI 1634 GGTCACC G^GT[ACGT]ACC
CelII 2374 GCTGAGC GC^T[ACGT]AGC
CfoI 1945 GCGC GCG^C
DrdI 1542 GACTACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2385 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3432 CTCTTC CTCTTC(1/4)
EcoO65I 1634 GGTCACC G^GT[ACGT]ACC
HapII 3245 CCGG C^CGG
HhaI 1945 GCGC GCG^C
HinP1I 1945 GCGC G^CGC
HpaII 3245 CCGG C^CGG
Ksp632I 3432 CTCTTC CTCTTC(1/4)
MspI 3245 CCGG C^CGG
NcoI 1562 CCATGG C^CATGG
PshBI 4299 ATTAAT AT^TAAT
Psp1406I 3071 AACGTT AA^CGTT
PvuII 434 CAGCTG CAG^CTG
SapI 3431 GCTCTTC GCTCTTC(1/4)
ScaI 4057 AGTACT AGT^ACT
SpeI 3813 ACTAGT A^CTAGT
XcmI 1655 CCACTATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 1311 GTGCAC G^TGCAC
1745 GTGCAC
AocI 2435 CCTGAGG CC^T[ACGT]AGG
3714 CCTTAGG
ApaLI 1311 GTGCAC G^TGCAC
1745 GTGCAC
Asp700I 398 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
892 GAAGTGGTTC
AvaII 1443 GGACC G^G[AT]CC
2365 GGACC
BalI 1642 TGGCCA TGG^CCA
2519 TGGCCA
BanI 780 GGTGCC G^G[CT][GA]CC
1515 GGCACC
BbsI 1671 GAAGAC GAAGAC(2/6)
255 GTCTTC
BpuAI 1671 GAAGAC GAAGAC(2/6)
255 GTCTTC
BsaAI 1838 TACGTG [CT]AC^GT[GA]
2875 TACGTG
BsaI 2865 GAGACC GGTCTC(1/5)
1501 GGTCTC
BsgI 3413 CTGCAC GTGCAG(16/14)
1602 GTGCAG
BspMI 168 GCAGGT ACCTGC(4/8)
1604 GCAGGT
Bsu36I 2435 CCTGAGG CC^T[ACGT]AGG
3714 CCTTAGG
EaeI 1642 TGGCCA [CT]^GGCC[GA]
2519 TGGCCA
Eco81I 2435 CCTGAGG CC^T[ACGT]AGG
3714 CCTTAGG
EcoRI 142 GAATTC G^AATTC
3643 GAATTC
HindIII 3041 AAGCTT A^AGCTT
3775 AAGCTT
HpaI 1902 GTTAAC GTT^AAC
3127 GTTAAC
MluNI 1642 TGGCCA TGG^CCA
2519 TGGCCA
MscI 1642 TGGCCA TGG^CCA
2519 TGGCCA
MspA1I 133 CAGCGG C[AC]G^C[GT]G
434 CAGCTG
PflMI 617 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1142 CCATGACTTGG
SacI 1258 GAGCTC GAGCT^C
1783 GAGCTC
StuI 1573 AGGCCT AGG^CCT
2359 AGGCCT
SwaI 3849 ATTTAAAT ATTT^AAAT
3967 ATTTAAAT
TaqI 239 TCGA T^CGA
1469 TCGA
TthHB8I 239 TCGA T^CGA
1469 TCGA
Van91I 617 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1142 CCATGACTTGG
XmnI 398 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
892 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwI AlwNI ApoI AspHI BanII
BbvI BciVI BclI BfaI BfrI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII