##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AflII Alw44I Aor51HI
ApaLI AscI AviII AvrII BalI
BamHI BbvCI BcgI BfrI BlnI
BsiWI BsmI BspDI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HindIII HpaI MluI
MluNI MscI NdeI NheI NotI
NruI NspV PacI PmeI Psp1406I
PvuI RsrII SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3807 GGTACC G^GTACC
AgeI 1305 ACCGGT A^CCGGT
AhdI 3371 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3506 CCTCAGG CC^T[ACGT]AGG
AseI 520 ATTAAT AT^TAAT
AsnI 520 ATTAAT AT^TAAT
Asp718I 3807 GGTACC G^GTACC
AspEI 3371 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2657 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 651 TGATCA T^GATCA
BsaBI 1147 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2700 GAGACC GGTCTC(1/5)
BsiEI 336 CGACCG CG[GA][CT]^CG
BsmBI 153 CGTCTC CGTCTC(1/5)
BspLU11I 4473 ACATGT A^CATGT
BstAPI 2323 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3506 CCTCAGG CC^T[ACGT]AGG
DrdI 3068 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3371 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3506 CCTCAGG CC^T[ACGT]AGG
EcoNI 3224 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2909 GAATTC G^AATTC
FbaI 651 TGATCA T^GATCA
KpnI 3807 GGTACC GGTAC^C
KspI 339 CCGCGG CCGC^GG
MamI 1147 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3776 CAATTG C^AATTG
MunI 3776 CAATTG C^AATTG
PaeR7I 2451 CTCGAG C^TCGAG
PflFI 2657 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1305 ACCGGT A^CCGGT
PshAI 763 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 520 ATTAAT AT^TAAT
PvuII 2363 CAGCTG CAG^CTG
SacI 3714 GAGCTC GAGCT^C
SacII 339 CCGCGG CCGC^GG
SalI 3627 GTCGAC G^TCGAC
Tth111I 2657 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2451 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 840 TCCGGA T^CCGGA
1993 TCCGGA
AflIII 1749 ACGTGT A^C[GA][CT]GT
4473 ACATGT
ApaI 2758 GGGCCC GGGCC^C
3013 GGGCCC
Asp700I 1634 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4694 GAACACCTTC
BbeI 1007 GGCGCC GGCGC^C
2022 GGCGCC
BbrPI 776 CACGTG CAC^GTG
1373 CACGTG
BciVI 3233 GGATAC GTATCC(6/5)
1886 GTATCC
BglI 1071 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2287 GCCTTCTTGGC
BglII 918 AGATCT A^GATCT
3675 AGATCT
BlpI 4167 GCTTAGC GC^T[ACGT]AGC
4218 GCTGAGC
Bpu1102I 4167 GCTTAGC GC^T[ACGT]AGC
4218 GCTGAGC
BseAI 840 TCCGGA T^CCGGA
1993 TCCGGA
BspEI 840 TCCGGA T^CCGGA
1993 TCCGGA
CelII 4167 GCTTAGC GC^T[ACGT]AGC
4218 GCTGAGC
DraI 3945 TTTAAA TTT^AAA
4373 TTTAAA
EaeI 14 TGGCCG [CT]^GGCC[GA]
4046 TGGCCG
EcoT22I 1763 ATGCAT ATGCA^T
3921 ATGCAT
HaeII 1007 GGCGCC [GA]GCGC^[CT]
2022 GGCGCC
KasI 1007 GGCGCC G^GCGCC
2022 GGCGCC
MroI 840 TCCGGA T^CCGGA
1993 TCCGGA
NaeI 81 GCCGGC GCC^GGC
1329 GCCGGC
NarI 1007 GGCGCC GG^CGCC
2022 GGCGCC
NgoMIV 81 GCCGGC G^CCGGC
1329 GCCGGC
NsiI 1763 ATGCAT ATGCA^T
3921 ATGCAT
PmaCI 776 CACGTG CAC^GTG
1373 CACGTG
PmlI 776 CACGTG CAC^GTG
1373 CACGTG
ScaI 1671 AGTACT AGT^ACT
2834 AGTACT
SexAI 3950 ACCTGGT A^CC[AT]GGT
4543 ACCAGGT
SfoI 1007 GGCGCC GGC^GCC
2022 GGCGCC
SmaI 1407 CCCGGG CCC^GGG
1437 CCCGGG
SphI 125 GCATGC GCATG^C
1765 GCATGC
SspI 1534 AATATT AAT^ATT
2178 AATATT
XmaCI 1407 CCCGGG C^CCGGG
1437 CCCGGG
XmaI 1407 CCCGGG C^CCGGG
1437 CCCGGG
XmnI 1634 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4694 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII