##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
BalI BbeI BbrPI BcgI BglI
BlpI BpmI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BsrBI BssHII BssSI BstAPI
BstEII BstPI CelII ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NarI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 11 GGCGTC G[GA]^CG[CT]C
AflII 1900 CTTAAG C^TTAAG
AflIII 2644 ACATGT A^C[GA][CT]GT
AocI 1029 CCTCAGG CC^T[ACGT]AGG
ApaI 29 GGGCCC GGGCC^C
AseI 4375 ATTAAT AT^TAAT
AsnI 4375 ATTAAT AT^TAAT
AspHI 1952 GAGCTC G[AT]GC[AT]^C
AvrII 3021 CCTAGG C^CTAGG
BamHI 2319 GGATCC G^GATCC
BclI 2262 TGATCA T^GATCA
BfrI 1900 CTTAAG C^TTAAG
BlnI 3021 CCTAGG C^CTAGG
BsaHI 11 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1952 GAGCTC G[AT]GC[AT]^C
BsmBI 474 GAGACG CGTCTC(1/5)
BsmI 972 GCATTC GAATGC(1/-1)
BspLU11I 2644 ACATGT A^CATGT
BsrDI 1182 CATTGC GCAATG(2/0)
BsrFI 8 GCCGGC [GA]^CCGG[CT]
Bst1107I 445 GTATAC GTA^TAC
BstBI 836 TTCGAA TT^CGAA
BstZ17I 445 GTATAC GTA^TAC
Bsu36I 1029 CCTCAGG CC^T[ACGT]AGG
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
DraII 2465 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1029 CCTCAGG CC^T[ACGT]AGG
EcoNI 1513 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2465 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 2262 TGATCA T^GATCA
HaeII 99 GGCGCT [GA]GCGC^[CT]
Hin1I 11 GGCGTC G[GA]^CG[CT]C
HpaI 2038 GTTAAC GTT^AAC
MslI 1668 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 8 GCCGGC GCC^GGC
NdeI 3413 CATATG CA^TATG
NgoMIV 8 GCCGGC G^CCGGC
NspV 836 TTCGAA TT^CGAA
PflMI 3949 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4375 ATTAAT AT^TAAT
SacI 1952 GAGCTC GAGCT^C
SalI 710 GTCGAC G^TCGAC
ScaI 2588 AGTACT AGT^ACT
SfuI 836 TTCGAA TT^CGAA
SpeI 4330 ACTAGT A^CTAGT
Van91I 3949 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4266 TCTAGA T^CTAGA
XcmI 1883 CCAGGATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 34 CGCG CG^CG
36 CGCG
BanI 90 GGTGCC G^G[CT][GA]CC
3161 GGTGCC
BanII 29 GGGCCC G[GA]GC[CT]^C
1952 GAGCTC
BbsI 1063 GTCTTC GAAGAC(2/6)
3959 GTCTTC
BbvCI 2367 CCTCAGC CCTCAGC(-5/-2)
3133 GCTGAGG
BcnI 27 CCGGG CC^[GC]GG
1622 CCCGG
BmyI 29 GGGCCC G[AGT]GC[ACT]^C
1952 GAGCTC
BpuAI 1063 GTCTTC GAAGAC(2/6)
3959 GTCTTC
BsaAI 483 TACGTG [CT]AC^GT[GA]
4430 TACGTG
BsaBI 988 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4204 GATTGAAATC
BsaI 1497 GAGACC GGTCTC(1/5)
2050 GAGACC
BseRI 2365 CTCCTC GAGGAG(10/8)
731 GAGGAG
BsgI 3450 CTGCAC GTGCAG(16/14)
154 GTGCAG
Bsp1286I 29 GGGCCC G[AGT]GC[ACT]^C
1952 GAGCTC
BstUI 34 CGCG CG^CG
36 CGCG
BstXI 804 CCATGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
949 CCAGTCAACTGG
EcoRI 1345 GAATTC G^AATTC
1427 GAATTC
EcoRV 1219 GATATC GAT^ATC
1354 GATATC
HgaI 12 GCGTC GACGC(5/10)
2375 GCGTC
MamI 988 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4204 GATTGAAATC
MvnI 34 CGCG CG^CG
36 CGCG
NciI 27 CCGGG CC^[GC]GG
1622 CCCGG
TaqI 711 TCGA T^CGA
837 TCGA
TthHB8I 711 TCGA T^CGA
837 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BbvI BciVI BfaI BglII BsaJI
BsiYI BslI BsmAI BsmFI Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI