##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AvrII BamHI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BsaBI BseAI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FseI
HindIII KspI MamI MfeI MluI
MroI MunI NdeI NotI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SnaBI SphI SspI
StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2005 GGTACC G^GTACC
AclI 2663 AACGTT AA^CGTT
Alw44I 3016 GTGCAC G^TGCAC
ApaLI 3016 GTGCAC G^TGCAC
Asp700I 1380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2005 GGTACC G^GTACC
AviII 4089 TGCGCA TGC^GCA
BclI 3720 TGATCA T^GATCA
BlpI 1275 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1275 GCTGAGC GC^T[ACGT]AGC
BsaAI 1308 CACGTA [CT]AC^GT[GA]
BsaWI 3331 ACCGGA [AT]^CCGG[AT]
BsiEI 1095 CGGCCG CG[GA][CT]^CG
BspHI 2988 TCATGA T^CATGA
BstEII 2822 GGTAACC G^GT[ACGT]ACC
BstPI 2822 GGTAACC G^GT[ACGT]ACC
BstYI 127 GGATCT [GA]^GATC[CT]
CelII 1275 GCTGAGC GC^T[ACGT]AGC
EagI 1095 CGGCCG C^GGCCG
EclXI 1095 CGGCCG C^GGCCG
Eco52I 1095 CGGCCG C^GGCCG
EcoO65I 2822 GGTAACC G^GT[ACGT]ACC
FbaI 3720 TGATCA T^GATCA
FspI 4089 TGCGCA TGC^GCA
HgaI 2303 GCGTC GACGC(5/10)
HpaI 4007 GTTAAC GTT^AAC
KpnI 2005 GGTACC GGTAC^C
MflI 127 GGATCT [GA]^GATC[CT]
NheI 2728 GCTAGC G^CTAGC
NruI 2545 TCGCGA TCG^CGA
PaeR7I 2554 CTCGAG C^TCGAG
Psp1406I 2663 AACGTT AA^CGTT
RcaI 2988 TCATGA T^CATGA
SacI 1278 GAGCTC GAGCT^C
SbfI 1353 CCTGCAGG CCTGCA^GG
SfiI 1489 GGCCCTAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2579 ACTAGT A^CTAGT
Sse8387I 1353 CCTGCAGG CCTGCA^GG
XcmI 503 CCAATGGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2554 CTCGAG C^TCGAG
XhoII 127 GGATCT [GA]^GATC[CT]
XmnI 1380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2293 GACGTC GACGT^C
3062 GACGTC
AflIII 1550 ACATGT A^C[GA][CT]GT
1988 ACATGT
AlwI 127 GGATC GGATC(4/5)
4399 GGATC
AocI 1492 CCTAAGG CC^T[ACGT]AGG
1564 CCTGAGG
BbeI 229 GGCGCC GGCGC^C
484 GGCGCC
BglI 1490 GCCCTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2040 GCCCTCCCGGC
BsaI 604 GAGACC GGTCTC(1/5)
700 GAGACC
Bsp1407I 1541 TGTACA T^GTACA
4016 TGTACA
BspLU11I 1550 ACATGT A^CATGT
1988 ACATGT
BspMI 1356 GCAGGT ACCTGC(4/8)
1901 GCAGGT
BsrBI 289 GAGCGG CCGCTC(-3/-3)
1666 GAGCGG
BsrGI 1541 TGTACA T^GTACA
4016 TGTACA
Bsu36I 1492 CCTAAGG CC^T[ACGT]AGG
1564 CCTGAGG
DraI 3302 TTTAAA TTT^AAA
3403 TTTAAA
Eco81I 1492 CCTAAGG CC^T[ACGT]AGG
1564 CCTGAGG
EcoNI 2403 CCTCTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3580 CCTTGCAGAGG
HincII 2383 GTCAAC GT[CT]^[GA]AC
4007 GTTAAC
HindII 2383 GTCAAC GT[CT]^[GA]AC
4007 GTTAAC
KasI 229 GGCGCC G^GCGCC
484 GGCGCC
NaeI 740 GCCGGC GCC^GGC
2420 GCCGGC
NarI 229 GGCGCC GG^CGCC
484 GGCGCC
NcoI 467 CCATGG C^CATGG
2516 CCATGG
NgoMIV 740 GCCGGC G^CCGGC
2420 GCCGGC
NspI 1550 ACATGT [GA]CATG^[CT]
1988 ACATGT
PflMI 2734 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4413 CCAGAGTGTGG
SfoI 229 GGCGCC GGC^GCC
484 GGCGCC
SmlI 621 CTCAAG C^T[CT][GA]AG
2554 CTCGAG
SspBI 1541 TGTACA T^GTACA
4016 TGTACA
Van91I 2734 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4413 CCAGAGTGTGG
XbaI 2658 TCTAGA T^CTAGA
3931 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI