##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AviII AvrII
BbeI BbrPI BclI BfrI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRI EcoT22I FbaI FspI KasI
KspI MfeI MluI MroI MunI
NarI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SapI SbfI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2672 GACGTC GACGT^C
Acc65I 1431 GGTACC G^GTACC
AccII 2505 CGCG CG^CG
AflIII 61 ACGTGT A^C[GA][CT]GT
AocI 1526 CCTGAGG CC^T[ACGT]AGG
Asp718I 1431 GGTACC G^GTACC
AspI 2648 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3247 GGATCC G^GATCC
BbsI 866 GTCTTC GAAGAC(2/6)
BcgI 569 GCAGGCGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2777 GGATAC GTATCC(6/5)
BpuAI 866 GTCTTC GAAGAC(2/6)
BsaBI 721 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 574 CGTCTC CGTCTC(1/5)
Bsp1407I 1773 TGTACA T^GTACA
BsrBI 2921 GAGCGG CCGCTC(-3/-3)
BsrGI 1773 TGTACA T^GTACA
BstUI 2505 CGCG CG^CG
BstXI 1319 CCAGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1526 CCTGAGG CC^T[ACGT]AGG
DrdI 3587 GACCTCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1526 CCTGAGG CC^T[ACGT]AGG
EcoRV 3035 GATATC GAT^ATC
FseI 596 GGCCGGCC GGCCGG^CC
HaeII 443 AGCGCC [GA]GCGC^[CT]
HindIII 1211 AAGCTT A^AGCTT
HpaI 854 GTTAAC GTT^AAC
KpnI 1431 GGTACC GGTAC^C
MamI 721 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2505 CGCG CG^CG
NdeI 1381 CATATG CA^TATG
NheI 493 GCTAGC G^CTAGC
PaeR7I 2273 CTCGAG C^TCGAG
PflFI 2648 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1865 CAGCTG CAG^CTG
SalI 2252 GTCGAC G^TCGAC
ScaI 2154 AGTACT AGT^ACT
SexAI 1800 ACCAGGT A^CC[AT]GGT
SfiI 2833 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3360 ACTAGT A^CTAGT
SspBI 1773 TGTACA T^GTACA
Tsp509I 2480 AATT ^AATT
Tth111I 2648 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2273 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2252 GTCGAC GT^[AC][GT]AC
2654 GTCTAC
AhdI 2521 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2672 GACGTCTGGTC
AlwNI 1568 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3284 CAGGGGCTG
AspEI 2521 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2672 GACGTCTGGTC
AvaI 2240 CCCGAG C^[CT]CG[GA]G
2273 CTCGAG
BglI 2076 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2834 GCCTGCTGGGC
BlpI 834 GCTCAGC GC^T[ACGT]AGC
3296 GCTGAGC
Bpu1102I 834 GCTCAGC GC^T[ACGT]AGC
3296 GCTGAGC
BsaI 426 GAGACC GGTCTC(1/5)
1586 GAGACC
BsoBI 2240 CCCGAG C^[CT]CG[GA]G
2273 CTCGAG
BspHI 2070 TCATGA T^CATGA
2571 TCATGA
BsrDI 294 GCAATG GCAATG(2/0)
2601 GCAATG
BsrFI 597 GCCGGC [GA]^CCGG[CT]
980 GCCGGC
BssSI 2090 CACGAG CACGAG(-5/-1)
3342 CTCGTG
BstAPI 1567 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3515 GCAGCAGATGC
BstEII 274 GGTGACC G^GT[ACGT]ACC
1099 GGTGACC
BstPI 274 GGTGACC G^GT[ACGT]ACC
1099 GGTGACC
CelII 834 GCTCAGC GC^T[ACGT]AGC
3296 GCTGAGC
Cfr10I 597 GCCGGC [GA]^CCGG[CT]
980 GCCGGC
DraIII 1553 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3809 CACGCCGTG
Eam1105I 2521 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2672 GACGTCTGGTC
EarI 1703 CTCTTC CTCTTC(1/4)
20 GAAGAG
EcoO65I 274 GGTGACC G^GT[ACGT]ACC
1099 GGTGACC
Ksp632I 1703 CTCTTC CTCTTC(1/4)
20 GAAGAG
NaeI 597 GCCGGC GCC^GGC
980 GCCGGC
NgoMIV 597 GCCGGC G^CCGGC
980 GCCGGC
RcaI 2070 TCATGA T^CATGA
2571 TCATGA
SmlI 1069 CTTGAG C^T[CT][GA]AG
2273 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII