##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AvrII BamHI BbrPI BcgI BciVI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspHI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoRI EcoRV
FseI HincII HindII HindIII HpaI
MluI MroI NaeI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2292 GACGTC GACGT^C
Acc65I 166 GGTACC G^GTACC
AccI 3972 GTCTAC GT^[AC][GT]AC
Alw44I 3498 GTGCAC G^TGCAC
ApaLI 3498 GTGCAC G^TGCAC
Asp700I 371 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 166 GGTACC G^GTACC
AspI 2666 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2611 TGCGCA TGC^GCA
BglII 1246 AGATCT A^GATCT
BsaAI 3637 TACGTG [CT]AC^GT[GA]
BsaBI 4090 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 4057 GCAGGT ACCTGC(4/8)
BstEII 1992 GGTTACC G^GT[ACGT]ACC
BstPI 1992 GGTTACC G^GT[ACGT]ACC
BstXI 790 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4173 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1505 CGGCCG C^GGCCG
EclXI 1505 CGGCCG C^GGCCG
Eco52I 1505 CGGCCG C^GGCCG
EcoO65I 1992 GGTTACC G^GT[ACGT]ACC
EcoT22I 3519 ATGCAT ATGCA^T
FspI 2611 TGCGCA TGC^GCA
KpnI 166 GGTACC GGTAC^C
KspI 610 CCGCGG CCGC^GG
MamI 4090 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1235 CAATTG C^AATTG
MunI 1235 CAATTG C^AATTG
NdeI 3830 CATATG CA^TATG
NsiI 3519 ATGCAT ATGCA^T
PflFI 2666 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1772 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 610 CCGCGG CCGC^GG
SexAI 187 ACCAGGT A^CC[AT]GGT
SphI 3707 GCATGC GCATG^C
StuI 598 AGGCCT AGG^CCT
Tth111I 2666 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 371 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1374 GAATTT [GA]^AATT[CT]
3433 GAATTT
AlwNI 320 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1770 CAGACCCTG
AocI 1413 CCTGAGG CC^T[ACGT]AGG
2277 CCTAAGG
ApaI 26 GGGCCC GGGCC^C
264 GGGCCC
ApoI 1374 GAATTT [GA]^AATT[CT]
3433 GAATTT
BbeI 62 GGCGCC GGCGC^C
2815 GGCGCC
BbvCI 3212 CCTCAGC CCTCAGC(-5/-2)
801 GCTGAGG
BclI 2441 TGATCA T^GATCA
2620 TGATCA
BglI 79 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1458 GCCATCTGGGC
BlpI 2885 GCTTAGC GC^T[ACGT]AGC
3508 GCTCAGC
Bpu1102I 2885 GCTTAGC GC^T[ACGT]AGC
3508 GCTCAGC
BsaWI 427 TCCGGT [AT]^CCGG[AT]
2245 ACCGGA
BsiEI 1505 CGGCCG CG[GA][CT]^CG
2879 CGACCG
BsmI 3626 GCATTC GAATGC(1/-1)
3952 GCATTC
BsrBI 2807 CCGCTC CCGCTC(-3/-3)
575 GAGCGG
BsrFI 868 ACCGGC [GA]^CCGG[CT]
2881 ACCGGC
BssHII 32 GCGCGC G^CGCGC
542 GCGCGC
BstAPI 2535 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3271 GCAACCCCTGC
Bsu36I 1413 CCTGAGG CC^T[ACGT]AGG
2277 CCTAAGG
CelII 2885 GCTTAGC GC^T[ACGT]AGC
3508 GCTCAGC
Cfr10I 868 ACCGGC [GA]^CCGG[CT]
2881 ACCGGC
DsaI 610 CCGCGG C^C[GA][CT]GG
3266 CCACGG
Eco81I 1413 CCTGAGG CC^T[ACGT]AGG
2277 CCTAAGG
FbaI 2441 TGATCA T^GATCA
2620 TGATCA
HgaI 1649 GACGC GACGC(5/10)
1184 GCGTC
KasI 62 GGCGCC G^GCGCC
2815 GGCGCC
NarI 62 GGCGCC GG^CGCC
2815 GGCGCC
PstI 776 CTGCAG CTGCA^G
3278 CTGCAG
SacI 669 GAGCTC GAGCT^C
3451 GAGCTC
SfoI 62 GGCGCC GGC^GCC
2815 GGCGCC
SmlI 672 CTCAAG C^T[CT][GA]AG
3670 CTTGAG
XcmI 1459 CCATCTGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3869 CCAATTCATGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI