##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI Bsp1407I BspEI
BspHI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII CpoI EcoRV
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2972 GTCTAC GT^[AC][GT]AC
AclI 3903 AACGTT AA^CGTT
AhdI 1591 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2645 GTGCAC G^TGCAC
ApaLI 2645 GTGCAC G^TGCAC
Asp700I 3925 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1591 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2945 CCTCAGC CCTCAGC(-5/-2)
BcgI 3598 CGACAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1881 GTATCC GTATCC(6/5)
BglII 2910 AGATCT A^GATCT
BsiEI 163 CGGCCG CG[GA][CT]^CG
BspDI 2966 ATCGAT AT^CGAT
BspLU11I 669 ACATGT A^CATGT
BsrDI 416 CATTGC GCAATG(2/0)
BstEII 2848 GGTGACC G^GT[ACGT]ACC
BstPI 2848 GGTGACC G^GT[ACGT]ACC
ClaI 2966 ATCGAT AT^CGAT
DraI 1178 TTTAAA TTT^AAA
DraIII 2223 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 884 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 163 CGGCCG C^GGCCG
Eam1105I 1591 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 163 CGGCCG C^GGCCG
Eco52I 163 CGGCCG C^GGCCG
EcoO65I 2848 GGTGACC G^GT[ACGT]ACC
EcoT22I 3104 ATGCAT ATGCA^T
HincII 3584 GTCAAC GT[CT]^[GA]AC
HindII 3584 GTCAAC GT[CT]^[GA]AC
NaeI 1982 GCCGGC GCC^GGC
NgoMIV 1982 GCCGGC G^CCGGC
NsiI 3104 ATGCAT ATGCA^T
Psp1406I 3903 AACGTT AA^CGTT
SapI 129 GCTCTTC GCTCTTC(1/4)
ScaI 3927 AGTACT AGT^ACT
SmaI 873 CCCGGG CCC^GGG
XmaCI 873 CCCGGG C^CCGGG
XmaI 873 CCCGGG C^CCGGG
XmnI 3925 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 669 ACATGT A^C[GA][CT]GT
2463 ACGTGT
AocI 1835 CCTCAGG CC^T[ACGT]AGG
3736 CCTAAGG
Aor51HI 173 AGCGCT AGC^GCT
3072 AGCGCT
BalI 2442 TGGCCA TGG^CCA
2718 TGGCCA
BbrPI 919 CACGTG CAC^GTG
2462 CACGTG
BclI 2979 TGATCA T^GATCA
3036 TGATCA
BfaI 1412 CTAG C^TAG
3917 CTAG
BsaI 3063 GAGACC GGTCTC(1/5)
3507 GAGACC
BsmBI 1714 CGTCTC CGTCTC(1/5)
909 GAGACG
BsrFI 1261 GCCGGT [GA]^CCGG[CT]
1982 GCCGGC
BssSI 245 CACGAG CACGAG(-5/-1)
1060 CTCGTG
BstXI 2085 CCACTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2721 CCAAACTGCTGG
Bsu36I 1835 CCTCAGG CC^T[ACGT]AGG
3736 CCTAAGG
Cfr10I 1261 GCCGGT [GA]^CCGG[CT]
1982 GCCGGC
Eco47III 173 AGCGCT AGC^GCT
3072 AGCGCT
Eco81I 1835 CCTCAGG CC^T[ACGT]AGG
3736 CCTAAGG
EcoNI 1765 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1831 CCTGCCTCAGG
FbaI 2979 TGATCA T^GATCA
3036 TGATCA
MaeI 1412 CTAG C^TAG
3917 CTAG
MluNI 2442 TGGCCA TGG^CCA
2718 TGGCCA
MscI 2442 TGGCCA TGG^CCA
2718 TGGCCA
NdeI 2886 CATATG CA^TATG
2956 CATATG
PmaCI 919 CACGTG CAC^GTG
2462 CACGTG
PmlI 919 CACGTG CAC^GTG
2462 CACGTG
SacI 127 GAGCTC GAGCT^C
3250 GAGCTC
SmlI 1502 CTCAAG C^T[CT][GA]AG
2254 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BamHI
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII