################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I CelII CpoI EcoRV FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NcoI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 2972 GTCTAC GT^[AC][GT]AC AclI 3903 AACGTT AA^CGTT AhdI 1591 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2645 GTGCAC G^TGCAC ApaLI 2645 GTGCAC G^TGCAC Asp700I 3925 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1591 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 2945 CCTCAGC CCTCAGC(-5/-2) BcgI 3598 CGACAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1881 GTATCC GTATCC(6/5) BglII 2910 AGATCT A^GATCT BsiEI 163 CGGCCG CG[GA][CT]^CG BspDI 2966 ATCGAT AT^CGAT BspLU11I 669 ACATGT A^CATGT BsrDI 416 CATTGC GCAATG(2/0) BstEII 2848 GGTGACC G^GT[ACGT]ACC BstPI 2848 GGTGACC G^GT[ACGT]ACC ClaI 2966 ATCGAT AT^CGAT DraI 1178 TTTAAA TTT^AAA DraIII 2223 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 884 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 163 CGGCCG C^GGCCG Eam1105I 1591 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 163 CGGCCG C^GGCCG Eco52I 163 CGGCCG C^GGCCG EcoO65I 2848 GGTGACC G^GT[ACGT]ACC EcoT22I 3104 ATGCAT ATGCA^T HincII 3584 GTCAAC GT[CT]^[GA]AC HindII 3584 GTCAAC GT[CT]^[GA]AC NaeI 1982 GCCGGC GCC^GGC NgoMIV 1982 GCCGGC G^CCGGC NsiI 3104 ATGCAT ATGCA^T Psp1406I 3903 AACGTT AA^CGTT SapI 129 GCTCTTC GCTCTTC(1/4) ScaI 3927 AGTACT AGT^ACT SmaI 873 CCCGGG CCC^GGG XmaCI 873 CCCGGG C^CCGGG XmaI 873 CCCGGG C^CCGGG XmnI 3925 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 669 ACATGT A^C[GA][CT]GT 2463 ACGTGT AocI 1835 CCTCAGG CC^T[ACGT]AGG 3736 CCTAAGG Aor51HI 173 AGCGCT AGC^GCT 3072 AGCGCT BalI 2442 TGGCCA TGG^CCA 2718 TGGCCA BbrPI 919 CACGTG CAC^GTG 2462 CACGTG BclI 2979 TGATCA T^GATCA 3036 TGATCA BfaI 1412 CTAG C^TAG 3917 CTAG BsaI 3063 GAGACC GGTCTC(1/5) 3507 GAGACC BsmBI 1714 CGTCTC CGTCTC(1/5) 909 GAGACG BsrFI 1261 GCCGGT [GA]^CCGG[CT] 1982 GCCGGC BssSI 245 CACGAG CACGAG(-5/-1) 1060 CTCGTG BstXI 2085 CCACTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2721 CCAAACTGCTGG Bsu36I 1835 CCTCAGG CC^T[ACGT]AGG 3736 CCTAAGG Cfr10I 1261 GCCGGT [GA]^CCGG[CT] 1982 GCCGGC Eco47III 173 AGCGCT AGC^GCT 3072 AGCGCT Eco81I 1835 CCTCAGG CC^T[ACGT]AGG 3736 CCTAAGG EcoNI 1765 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1831 CCTGCCTCAGG FbaI 2979 TGATCA T^GATCA 3036 TGATCA MaeI 1412 CTAG C^TAG 3917 CTAG MluNI 2442 TGGCCA TGG^CCA 2718 TGGCCA MscI 2442 TGGCCA TGG^CCA 2718 TGGCCA NdeI 2886 CATATG CA^TATG 2956 CATATG PmaCI 919 CACGTG CAC^GTG 2462 CACGTG PmlI 919 CACGTG CAC^GTG 2462 CACGTG SacI 127 GAGCTC GAGCT^C 3250 GAGCTC SmlI 1502 CTCAAG C^T[CT][GA]AG 2254 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AspI AvaI AvaII BamHI BanI BanII BbeI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII