##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AvaI AviII
AvrII BbeI BcgI BcnI BlnI
BlpI Bpu1102I BsaHI BseAI BseRI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KspI MluI
MroI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SphI Sse8387I
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3549 GGTACC G^GTACC
AccI 1226 GTCTAC GT^[AC][GT]AC
AflII 2157 CTTAAG C^TTAAG
AflIII 2183 ACGTGT A^C[GA][CT]GT
AgeI 4416 ACCGGT A^CCGGT
AocI 3285 CCTAAGG CC^T[ACGT]AGG
Asp718I 3549 GGTACC G^GTACC
BamHI 2330 GGATCC G^GATCC
BanI 3549 GGTACC G^G[CT][GA]CC
BanII 4144 GAGCCC G[GA]GC[CT]^C
BbrPI 655 CACGTG CAC^GTG
BbvCI 2238 GCTGAGG CCTCAGC(-5/-2)
BciVI 911 GTATCC GTATCC(6/5)
BfrI 2157 CTTAAG C^TTAAG
BglI 1914 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3073 GGTCTC GGTCTC(1/5)
BsgI 3472 GTGCAG GTGCAG(16/14)
BsiEI 4418 CGGTCG CG[GA][CT]^CG
BspMI 4778 ACCTGC ACCTGC(4/8)
BsrDI 4485 GCAATG GCAATG(2/0)
BsrFI 4416 ACCGGT [GA]^CCGG[CT]
BstAPI 2619 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3285 CCTAAGG CC^T[ACGT]AGG
Cfr10I 4416 ACCGGT [GA]^CCGG[CT]
DraII 3515 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eco81I 3285 CCTAAGG CC^T[ACGT]AGG
EcoO109I 3515 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 327 ATGCAT ATGCA^T
HincII 1673 GTTAAC GT[CT]^[GA]AC
HindII 1673 GTTAAC GT[CT]^[GA]AC
HpaI 1673 GTTAAC GTT^AAC
KpnI 3549 GGTACC GGTAC^C
MfeI 1359 CAATTG C^AATTG
MunI 1359 CAATTG C^AATTG
NsiI 327 ATGCAT ATGCA^T
NspI 325 ACATGC [GA]CATG^[CT]
PacI 4492 TTAATTAA TTAAT^TAA
PflMI 4047 CCATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4416 ACCGGT A^CCGGT
PmaCI 655 CACGTG CAC^GTG
PmlI 655 CACGTG CAC^GTG
PpuMI 3515 AGGTCCC [GA]G^G[AT]CC[CT]
ScaI 1513 AGTACT AGT^ACT
SnaBI 2776 TACGTA TAC^GTA
SpeI 1533 ACTAGT A^CTAGT
Tsp45I 4691 GTCAC ^GT[GC]AC
Van91I 4047 CCATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 1859 GCGG CCGC(-3/-1)
3186 GCGG
AspHI 3457 GAGCAC G[AT]GC[AT]^C
4509 GAGCAC
AvaII 1605 GGTCC G^G[AT]CC
3516 GGTCC
BalI 1363 TGGCCA TGG^CCA
1853 TGGCCA
BclI 2690 TGATCA T^GATCA
4349 TGATCA
BsaWI 1693 ACCGGA [AT]^CCGG[AT]
4416 ACCGGT
BsiHKAI 3457 GAGCAC G[AT]GC[AT]^C
4509 GAGCAC
BsmFI 3517 GTCCC GGGAC(10/14)
4128 GTCCC
Bsp1407I 1045 TGTACA T^GTACA
4572 TGTACA
BspHI 395 TCATGA T^CATGA
549 TCATGA
BsrGI 1045 TGTACA T^GTACA
4572 TGTACA
DsaI 2218 CCATGG C^C[GA][CT]GG
2677 CCATGG
EaeI 1363 TGGCCA [CT]^GGCC[GA]
1853 TGGCCA
EcoRI 2742 GAATTC G^AATTC
3447 GAATTC
FbaI 2690 TGATCA T^GATCA
4349 TGATCA
HapII 1694 CCGG C^CGG
4417 CCGG
HindIII 1166 AAGCTT A^AGCTT
2820 AAGCTT
HpaII 1694 CCGG C^CGG
4417 CCGG
MluNI 1363 TGGCCA TGG^CCA
1853 TGGCCA
MscI 1363 TGGCCA TGG^CCA
1853 TGGCCA
MspI 1694 CCGG C^CGG
4417 CCGG
NcoI 2218 CCATGG C^CATGG
2677 CCATGG
NdeI 410 CATATG CA^TATG
2694 CATATG
PstI 829 CTGCAG CTGCA^G
3418 CTGCAG
PvuII 859 CAGCTG CAG^CTG
946 CAGCTG
RcaI 395 TCATGA T^CATGA
549 TCATGA
SmlI 2157 CTTAAG C^T[CT][GA]AG
3220 CTCAAG
SspBI 1045 TGTACA T^GTACA
4572 TGTACA
StuI 1332 AGGCCT AGG^CCT
4630 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaBI BsaJI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TfiI Tru9I TseI Tsp509I
TspRI XcmI XhoII XmnI