##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BamHI BbeI BbrPI BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII KasI MamI MfeI MluI
MroI MunI NarI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SfoI SgrAI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1858 GACGTC GACGT^C
Acc65I 259 GGTACC G^GTACC
AccI 2881 GTCTAC GT^[AC][GT]AC
AcsI 2416 AAATTC [GA]^AATT[CT]
AflII 2922 CTTAAG C^TTAAG
Alw44I 2341 GTGCAC G^TGCAC
ApaLI 2341 GTGCAC G^TGCAC
ApoI 2416 AAATTC [GA]^AATT[CT]
Asp700I 2019 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 259 GGTACC G^GTACC
AspI 1939 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1233 GCAGGCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2678 GTATCC GTATCC(6/5)
BfrI 2922 CTTAAG C^TTAAG
BglII 2405 AGATCT A^GATCT
BsaAI 2319 CACGTA [CT]AC^GT[GA]
BsaWI 3322 TCCGGT [AT]^CCGG[AT]
BsrFI 1487 GCCGGC [GA]^CCGG[CT]
BssSI 2796 CACGAG CACGAG(-5/-1)
BstBI 2842 TTCGAA TT^CGAA
Cfr10I 1487 GCCGGC [GA]^CCGG[CT]
DraI 4216 TTTAAA TTT^AAA
DraIII 1835 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
HpaI 3981 GTTAAC GTT^AAC
KpnI 259 GGTACC GGTAC^C
KspI 795 CCGCGG CCGC^GG
NaeI 1487 GCCGGC GCC^GGC
NgoMIV 1487 GCCGGC G^CCGGC
NheI 335 GCTAGC G^CTAGC
NspV 2842 TTCGAA TT^CGAA
PflFI 1939 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2446 GAGCTC GAGCT^C
SacII 795 CCGCGG CCGC^GG
SapI 1609 GCTCTTC GCTCTTC(1/4)
SbfI 2784 CCTGCAGG CCTGCA^GG
ScaI 4080 AGTACT AGT^ACT
SfiI 1371 GGCCACTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2842 TTCGAA TT^CGAA
Sse8387I 2784 CCTGCAGG CCTGCA^GG
Tth111I 1939 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2019 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1858 GACGTC G[GA]^CG[CT]C
1974 GGCGTC
AvrII 864 CCTAGG C^CTAGG
4175 CCTAGG
BbsI 2056 GTCTTC GAAGAC(2/6)
2839 GTCTTC
BbvCI 1864 CCTCAGC CCTCAGC(-5/-2)
2213 CCTCAGC
BclI 561 TGATCA T^GATCA
2708 TGATCA
BlnI 864 CCTAGG C^CTAGG
4175 CCTAGG
BpuAI 2056 GTCTTC GAAGAC(2/6)
2839 GTCTTC
BsaHI 1858 GACGTC G[GA]^CG[CT]C
1974 GGCGTC
BsmBI 1998 CGTCTC CGTCTC(1/5)
2586 CGTCTC
Bsp1407I 437 TGTACA T^GTACA
527 TGTACA
BspHI 564 TCATGA T^CATGA
1568 TCATGA
BspLU11I 752 ACATGT A^CATGT
1889 ACATGT
BsrDI 2253 CATTGC GCAATG(2/0)
2829 CATTGC
BsrGI 437 TGTACA T^GTACA
527 TGTACA
FbaI 561 TGATCA T^GATCA
2708 TGATCA
Hin1I 1858 GACGTC G[GA]^CG[CT]C
1974 GGCGTC
HincII 236 GTTGAC GT[CT]^[GA]AC
3981 GTTAAC
HindII 236 GTTGAC GT[CT]^[GA]AC
3981 GTTAAC
MaeII 1859 ACGT A^CGT
2320 ACGT
NdeI 1689 CATATG CA^TATG
4073 CATATG
PflMI 801 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3803 CCAGCCTCTGG
RcaI 564 TCATGA T^CATGA
1568 TCATGA
SexAI 915 ACCAGGT A^CC[AT]GGT
1940 ACCAGGT
SspBI 437 TGTACA T^GTACA
527 TGTACA
TaqI 2843 TCGA T^CGA
3629 TCGA
TthHB8I 2843 TCGA T^CGA
3629 TCGA
Van91I 801 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3803 CCAGCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmaCI XmaI