################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AviII BamHI BbeI BbrPI BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII KasI MamI MfeI MluI MroI MunI NarI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SfoI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1858 GACGTC GACGT^C Acc65I 259 GGTACC G^GTACC AccI 2881 GTCTAC GT^[AC][GT]AC AcsI 2416 AAATTC [GA]^AATT[CT] AflII 2922 CTTAAG C^TTAAG Alw44I 2341 GTGCAC G^TGCAC ApaLI 2341 GTGCAC G^TGCAC ApoI 2416 AAATTC [GA]^AATT[CT] Asp700I 2019 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 259 GGTACC G^GTACC AspI 1939 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 1233 GCAGGCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2678 GTATCC GTATCC(6/5) BfrI 2922 CTTAAG C^TTAAG BglII 2405 AGATCT A^GATCT BsaAI 2319 CACGTA [CT]AC^GT[GA] BsaWI 3322 TCCGGT [AT]^CCGG[AT] BsrFI 1487 GCCGGC [GA]^CCGG[CT] BssSI 2796 CACGAG CACGAG(-5/-1) BstBI 2842 TTCGAA TT^CGAA Cfr10I 1487 GCCGGC [GA]^CCGG[CT] DraI 4216 TTTAAA TTT^AAA DraIII 1835 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG HpaI 3981 GTTAAC GTT^AAC KpnI 259 GGTACC GGTAC^C KspI 795 CCGCGG CCGC^GG NaeI 1487 GCCGGC GCC^GGC NgoMIV 1487 GCCGGC G^CCGGC NheI 335 GCTAGC G^CTAGC NspV 2842 TTCGAA TT^CGAA PflFI 1939 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 2446 GAGCTC GAGCT^C SacII 795 CCGCGG CCGC^GG SapI 1609 GCTCTTC GCTCTTC(1/4) SbfI 2784 CCTGCAGG CCTGCA^GG ScaI 4080 AGTACT AGT^ACT SfiI 1371 GGCCACTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2842 TTCGAA TT^CGAA Sse8387I 2784 CCTGCAGG CCTGCA^GG Tth111I 1939 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 2019 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 1858 GACGTC G[GA]^CG[CT]C 1974 GGCGTC AvrII 864 CCTAGG C^CTAGG 4175 CCTAGG BbsI 2056 GTCTTC GAAGAC(2/6) 2839 GTCTTC BbvCI 1864 CCTCAGC CCTCAGC(-5/-2) 2213 CCTCAGC BclI 561 TGATCA T^GATCA 2708 TGATCA BlnI 864 CCTAGG C^CTAGG 4175 CCTAGG BpuAI 2056 GTCTTC GAAGAC(2/6) 2839 GTCTTC BsaHI 1858 GACGTC G[GA]^CG[CT]C 1974 GGCGTC BsmBI 1998 CGTCTC CGTCTC(1/5) 2586 CGTCTC Bsp1407I 437 TGTACA T^GTACA 527 TGTACA BspHI 564 TCATGA T^CATGA 1568 TCATGA BspLU11I 752 ACATGT A^CATGT 1889 ACATGT BsrDI 2253 CATTGC GCAATG(2/0) 2829 CATTGC BsrGI 437 TGTACA T^GTACA 527 TGTACA FbaI 561 TGATCA T^GATCA 2708 TGATCA Hin1I 1858 GACGTC G[GA]^CG[CT]C 1974 GGCGTC HincII 236 GTTGAC GT[CT]^[GA]AC 3981 GTTAAC HindII 236 GTTGAC GT[CT]^[GA]AC 3981 GTTAAC MaeII 1859 ACGT A^CGT 2320 ACGT NdeI 1689 CATATG CA^TATG 4073 CATATG PflMI 801 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3803 CCAGCCTCTGG RcaI 564 TCATGA T^CATGA 1568 TCATGA SexAI 915 ACCAGGT A^CC[AT]GGT 1940 ACCAGGT SspBI 437 TGTACA T^GTACA 527 TGTACA TaqI 2843 TCGA T^CGA 3629 TCGA TthHB8I 2843 TCGA T^CGA 3629 TCGA Van91I 801 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3803 CCAGCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI