##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AflII AgeI AscI AvaI
AviII BbeI BciVI BfrI BglI
BsaBI BsiEI BsmBI BsoBI BspDI
BspHI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoNI EcoRV EcoT22I
FseI FspI HpaI KasI MamI
MluI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RcaI RsrII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 2158 GGTACC G^GTACC
AccIII 1239 TCCGGA T^CCGGA
AclI 3051 AACGTT AA^CGTT
AcyI 513 GACGCC G[GA]^CG[CT]C
AflIII 2590 ACATGT A^C[GA][CT]GT
AhdI 1425 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4346 CCTCAGG CC^T[ACGT]AGG
AseI 3854 ATTAAT AT^TAAT
AsnI 3854 ATTAAT AT^TAAT
Asp700I 101 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2158 GGTACC G^GTACC
AspEI 1425 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2214 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4040 GGATCC G^GATCC
BbrPI 3883 CACGTG CAC^GTG
BbsI 1017 GAAGAC GAAGAC(2/6)
BclI 2110 TGATCA T^GATCA
BpuAI 1017 GAAGAC GAAGAC(2/6)
BsaHI 513 GACGCC G[GA]^CG[CT]C
BsaWI 1239 TCCGGA [AT]^CCGG[AT]
BseAI 1239 TCCGGA T^CCGGA
BsiWI 1957 CGTACG C^GTACG
Bsp1407I 1486 TGTACA T^GTACA
BspEI 1239 TCCGGA T^CCGGA
BspLU11I 2590 ACATGT A^CATGT
BsrFI 516 GCCGGC [GA]^CCGG[CT]
BsrGI 1486 TGTACA T^GTACA
BstAPI 375 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1640 GGTGACC G^GT[ACGT]ACC
BstPI 1640 GGTGACC G^GT[ACGT]ACC
Bsu36I 4346 CCTCAGG CC^T[ACGT]AGG
Cfr10I 516 GCCGGC [GA]^CCGG[CT]
DrdI 852 GACATTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1425 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4346 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1640 GGTGACC G^GT[ACGT]ACC
FbaI 2110 TGATCA T^GATCA
Hin1I 513 GACGCC G[GA]^CG[CT]C
HincII 573 GTCAAC GT[CT]^[GA]AC
HindII 573 GTCAAC GT[CT]^[GA]AC
KpnI 2158 GGTACC GGTAC^C
KspI 209 CCGCGG CCGC^GG
MfeI 137 CAATTG C^AATTG
MroI 1239 TCCGGA T^CCGGA
MunI 137 CAATTG C^AATTG
NaeI 516 GCCGGC GCC^GGC
NdeI 1177 CATATG CA^TATG
NgoMIV 516 GCCGGC G^CCGGC
PflFI 2214 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3883 CACGTG CAC^GTG
PmlI 3883 CACGTG CAC^GTG
PshBI 3854 ATTAAT AT^TAAT
Psp1406I 3051 AACGTT AA^CGTT
SacI 2670 GAGCTC GAGCT^C
SacII 209 CCGCGG CCGC^GG
SapI 1361 GAAGAGC GCTCTTC(1/4)
SbfI 4456 CCTGCAGG CCTGCA^GG
SmlI 4300 CTCAAG C^T[CT][GA]AG
Sse8387I 4456 CCTGCAGG CCTGCA^GG
SspBI 1486 TGTACA T^GTACA
SspI 900 AATATT AAT^ATT
StuI 4272 AGGCCT AGG^CCT
Tth111I 2214 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 101 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2259 GTCTAC GT^[AC][GT]AC
3268 GTCTAC
Alw44I 2053 GTGCAC G^TGCAC
4469 GTGCAC
Aor51HI 2165 AGCGCT AGC^GCT
3864 AGCGCT
ApaLI 2053 GTGCAC G^TGCAC
4469 GTGCAC
AvrII 149 CCTAGG C^CTAGG
4110 CCTAGG
BalI 1568 TGGCCA TGG^CCA
2518 TGGCCA
BanI 1826 GGTGCC G^G[CT][GA]CC
2158 GGTACC
BbvCI 4275 CCTCAGC CCTCAGC(-5/-2)
35 GCTGAGG
BlnI 149 CCTAGG C^CTAGG
4110 CCTAGG
BsaAI 2262 TACGTG [CT]AC^GT[GA]
3883 CACGTG
BsaI 1243 GAGACC GGTCTC(1/5)
4451 GAGACC
BsrDI 1106 CATTGC GCAATG(2/0)
2386 CATTGC
BstXI 1627 CCAGCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3991 CCAGGGCAGTGG
DraI 2680 TTTAAA TTT^AAA
2693 TTTAAA
DraIII 1317 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3215 CACAGAGTG
Eco47III 2165 AGCGCT AGC^GCT
3864 AGCGCT
EcoRI 1716 GAATTC G^AATTC
2789 GAATTC
FauI 3962 CCCGC CCCGC(4/6)
4419 CCCGC
HgaI 513 GACGC GACGC(5/10)
2027 GACGC
MluNI 1568 TGGCCA TGG^CCA
2518 TGGCCA
MscI 1568 TGGCCA TGG^CCA
2518 TGGCCA
NspI 2590 ACATGT [GA]CATG^[CT]
3199 GCATGT
SexAI 1504 ACCAGGT A^CC[AT]GGT
1762 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BanII BbvI BcgI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII