##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII BbrPI BbsI BciVI
BglII BpuAI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SexAI SfiI SfuI
SgrAI SnaBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 911 GGTACC G^GTACC
AccI 2128 GTCTAC GT^[AC][GT]AC
AcsI 748 AAATTC [GA]^AATT[CT]
AflII 3378 CTTAAG C^TTAAG
ApoI 748 AAATTC [GA]^AATT[CT]
Asp718I 911 GGTACC G^GTACC
AspI 182 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 726 CCTAGG C^CTAGG
BamHI 1929 GGATCC G^GATCC
BcgI 500 CGAAGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2983 TGATCA T^GATCA
BfrI 3378 CTTAAG C^TTAAG
BlnI 726 CCTAGG C^CTAGG
Bsp1407I 2054 TGTACA T^GTACA
BsrFI 1391 GCCGGC [GA]^CCGG[CT]
BsrGI 2054 TGTACA T^GTACA
BstAPI 1937 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3558 GGTGACC G^GT[ACGT]ACC
BstPI 3558 GGTGACC G^GT[ACGT]ACC
Cfr10I 1391 GCCGGC [GA]^CCGG[CT]
DraIII 214 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3558 GGTGACC G^GT[ACGT]ACC
FbaI 2983 TGATCA T^GATCA
HgaI 147 GCGTC GACGC(5/10)
KpnI 911 GGTACC GGTAC^C
KspI 1163 CCGCGG CCGC^GG
MseI 3379 TTAA T^TAA
MslI 2737 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1391 GCCGGC GCC^GGC
NgoMIV 1391 GCCGGC G^CCGGC
PflFI 182 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1163 CCGCGG CCGC^GG
SbfI 1575 CCTGCAGG CCTGCA^GG
SpeI 4051 ACTAGT A^CTAGT
SphI 78 GCATGC GCATG^C
Sse8387I 1575 CCTGCAGG CCTGCA^GG
SspBI 2054 TGTACA T^GTACA
TaqI 1981 TCGA T^CGA
Tru9I 3379 TTAA T^TAA
Tth111I 182 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1981 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1317 ACATGT A^C[GA][CT]GT
1712 ACATGT
Alw44I 3531 GTGCAC G^TGCAC
4019 GTGCAC
ApaLI 3531 GTGCAC G^TGCAC
4019 GTGCAC
BbeI 1388 GGCGCC GGCGC^C
3578 GGCGCC
BsaWI 682 ACCGGA [AT]^CCGG[AT]
1107 ACCGGA
BspLU11I 1317 ACATGT A^CATGT
1712 ACATGT
BssSI 3520 CACGAG CACGAG(-5/-1)
38 CTCGTG
BstYI 1929 GGATCC [GA]^GATC[CT]
3216 AGATCC
DsaI 248 CCACGG C^C[GA][CT]GG
1163 CCGCGG
KasI 1388 GGCGCC G^GCGCC
3578 GGCGCC
MaeII 32 ACGT A^CGT
2694 ACGT
MflI 1929 GGATCC [GA]^GATC[CT]
3216 AGATCC
NarI 1388 GGCGCC GG^CGCC
3578 GGCGCC
SapI 4253 GCTCTTC GCTCTTC(1/4)
4314 GCTCTTC
ScaI 2919 AGTACT AGT^ACT
4136 AGTACT
SfoI 1388 GGCGCC GGC^GCC
3578 GGCGCC
StuI 1580 AGGCCT AGG^CCT
1976 AGGCCT
XhoII 1929 GGATCC [GA]^GATC[CT]
3216 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TfiI TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XmaCI XmaI