##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Aor51HI AscI Asp700I Asp718I AspI
AviII BciVI BfrI BsaBI BseAI
BsiWI BsmBI BspDI BspEI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eco47III EcoO65I EcoRV
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SapI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AgeI 2500 ACCGGT A^CCGGT
AhdI 541 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2379 GGGCCC GGGCC^C
AseI 897 ATTAAT AT^TAAT
AsnI 897 ATTAAT AT^TAAT
AspEI 541 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 64 GGCGCC GGCGC^C
BbrPI 526 CACGTG CAC^GTG
BclI 2158 TGATCA T^GATCA
BsaAI 526 CACGTG [CT]AC^GT[GA]
BsiEI 3043 CGGCCG CG[GA][CT]^CG
BspHI 1246 TCATGA T^CATGA
BssHII 85 GCGCGC G^CGCGC
DraIII 3499 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3043 CGGCCG C^GGCCG
Eam1105I 541 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3043 CGGCCG C^GGCCG
Eco52I 3043 CGGCCG C^GGCCG
EcoNI 2384 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3899 GAATTC G^AATTC
EcoT22I 4127 ATGCAT ATGCA^T
FbaI 2158 TGATCA T^GATCA
KasI 64 GGCGCC G^GCGCC
NaeI 1945 GCCGGC GCC^GGC
NarI 64 GGCGCC GG^CGCC
NcoI 745 CCATGG C^CATGG
NdeI 3354 CATATG CA^TATG
NgoMIV 1945 GCCGGC G^CCGGC
NsiI 4127 ATGCAT ATGCA^T
PinAI 2500 ACCGGT A^CCGGT
PmaCI 526 CACGTG CAC^GTG
PmlI 526 CACGTG CAC^GTG
PshBI 897 ATTAAT AT^TAAT
RcaI 1246 TCATGA T^CATGA
SacI 2073 GAGCTC GAGCT^C
SalI 4288 GTCGAC G^TCGAC
SbfI 2603 CCTGCAGG CCTGCA^GG
SfiI 3178 GGCCTGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 64 GGCGCC GGC^GCC
SmaI 59 CCCGGG CCC^GGG
SmlI 1072 CTCAAG C^T[CT][GA]AG
Sse8387I 2603 CCTGCAGG CCTGCA^GG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 3095 GTCTAC GT^[AC][GT]AC
4288 GTCGAC
AcyI 64 GGCGCC G[GA]^CG[CT]C
2430 GACGCC
AflIII 1367 ACATGT A^C[GA][CT]GT
2045 ACATGT
Alw44I 2238 GTGCAC G^TGCAC
2346 GTGCAC
ApaLI 2238 GTGCAC G^TGCAC
2346 GTGCAC
AvrII 155 CCTAGG C^CTAGG
2036 CCTAGG
BamHI 724 GGATCC G^GATCC
2562 GGATCC
BlnI 155 CCTAGG C^CTAGG
2036 CCTAGG
BlpI 3519 GCTCAGC GC^T[ACGT]AGC
3735 GCTCAGC
Bpu1102I 3519 GCTCAGC GC^T[ACGT]AGC
3735 GCTCAGC
BsaHI 64 GGCGCC G[GA]^CG[CT]C
2430 GACGCC
BsaWI 401 TCCGGT [AT]^CCGG[AT]
2500 ACCGGT
BsmI 3397 GAATGC GAATGC(1/-1)
2335 GCATTC
BspLU11I 1367 ACATGT A^CATGT
2045 ACATGT
BssSI 2679 CACGAG CACGAG(-5/-1)
1190 CTCGTG
BstAPI 381 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3414 GCAGGGCCTGC
CelII 3519 GCTCAGC GC^T[ACGT]AGC
3735 GCTCAGC
DraI 1175 TTTAAA TTT^AAA
4074 TTTAAA
Hin1I 64 GGCGCC G[GA]^CG[CT]C
2430 GACGCC
MaeII 527 ACGT A^CGT
1410 ACGT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII