##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BciVI BglI BlnI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BsrBI BsrFI Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DrdI Eam1105I Eco47III EcoNI EcoRI
EcoRV FseI FspI HaeII KasI
KpnI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2862 GTAGAC GT^[AC][GT]AC
AlwNI 5206 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 3369 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2328 TGGCCA TGG^CCA
BanII 329 GAGCTC G[GA]GC[CT]^C
BbsI 760 GTCTTC GAAGAC(2/6)
BcgI 86 GCAGAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2054 TGATCA T^GATCA
BglII 4611 AGATCT A^GATCT
BlpI 507 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 507 GCTCAGC GC^T[ACGT]AGC
BpuAI 760 GTCTTC GAAGAC(2/6)
BsiEI 2700 CGGCCG CG[GA][CT]^CG
BspLU11I 1740 ACATGT A^CATGT
BssHII 23 GCGCGC G^CGCGC
BssSI 5497 CTCGTG CACGAG(-5/-1)
BstEII 4029 GGTAACC G^GT[ACGT]ACC
BstPI 4029 GGTAACC G^GT[ACGT]ACC
CelII 507 GCTCAGC GC^T[ACGT]AGC
DraIII 176 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2700 CGGCCG C^GGCCG
EclXI 2700 CGGCCG C^GGCCG
Eco52I 2700 CGGCCG C^GGCCG
EcoO65I 4029 GGTAACC G^GT[ACGT]ACC
FauI 3590 CCCGC CCCGC(4/6)
FbaI 2054 TGATCA T^GATCA
HpaI 3670 GTTAAC GTT^AAC
MfeI 1439 CAATTG C^AATTG
MluNI 2328 TGGCCA TGG^CCA
MscI 2328 TGGCCA TGG^CCA
MunI 1439 CAATTG C^AATTG
NcoI 4025 CCATGG C^CATGG
PflFI 3369 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 329 GAGCTC GAGCT^C
SpeI 889 ACTAGT A^CTAGT
SphI 3271 GCATGC GCATG^C
StuI 3626 AGGCCT AGG^CCT
Tth111I 3369 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 479 TCTAGA T^CTAGA
XcmI 5570 CCATCTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 4112 GACGCC G[GA]^CG[CT]C
4905 GACGCC
AseI 1839 ATTAAT AT^TAAT
5259 ATTAAT
AsnI 1839 ATTAAT AT^TAAT
5259 ATTAAT
Asp700I 1970 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2967 GAAAGCATTC
BbrPI 654 CACGTG CAC^GTG
1810 CACGTG
BbvCI 2236 CCTCAGC CCTCAGC(-5/-2)
3463 CCTCAGC
BsaAI 654 CACGTG [CT]AC^GT[GA]
1810 CACGTG
BsaHI 4112 GACGCC G[GA]^CG[CT]C
4905 GACGCC
BsaWI 367 TCCGGT [AT]^CCGG[AT]
2529 ACCGGA
BsgI 170 CTGCAC GTGCAG(16/14)
3425 CTGCAC
Bsp1407I 1737 TGTACA T^GTACA
2723 TGTACA
BsrGI 1737 TGTACA T^GTACA
2723 TGTACA
BstXI 195 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3608 CCAAAGTGCTGG
BstYI 3412 GGATCT [GA]^GATC[CT]
4611 AGATCT
DraII 3804 AGGCCCT [GA]G^G[ACGT]CC[CT]
4649 AGGCCCT
EcoO109I 3804 AGGCCCT [GA]G^G[ACGT]CC[CT]
4649 AGGCCCT
Hin1I 4112 GACGCC G[GA]^CG[CT]C
4905 GACGCC
HincII 1408 GTTGAC GT[CT]^[GA]AC
3670 GTTAAC
HindII 1408 GTTGAC GT[CT]^[GA]AC
3670 GTTAAC
MflI 3412 GGATCT [GA]^GATC[CT]
4611 AGATCT
PflMI 2207 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5540 CCAGTTGGTGG
PmaCI 654 CACGTG CAC^GTG
1810 CACGTG
PmlI 654 CACGTG CAC^GTG
1810 CACGTG
PshBI 1839 ATTAAT AT^TAAT
5259 ATTAAT
ScaI 111 AGTACT AGT^ACT
1387 AGTACT
SspBI 1737 TGTACA T^GTACA
2723 TGTACA
Van91I 2207 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5540 CCAGTTGGTGG
XhoII 3412 GGATCT [GA]^GATC[CT]
4611 AGATCT
XmnI 1970 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2967 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AocI ApoI
AspHI AvaI AvaII BanI BbvI
BcnI BfaI BfrI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI Eco81I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI