################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BamHI BbeI BciVI BglI BlnI BsaBI BseAI BsiWI BspDI BspEI BspHI BsrBI BsrFI Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV FseI FspI HaeII KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 2862 GTAGAC GT^[AC][GT]AC AlwNI 5206 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 3369 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2328 TGGCCA TGG^CCA BanII 329 GAGCTC G[GA]GC[CT]^C BbsI 760 GTCTTC GAAGAC(2/6) BcgI 86 GCAGAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2054 TGATCA T^GATCA BglII 4611 AGATCT A^GATCT BlpI 507 GCTCAGC GC^T[ACGT]AGC Bpu1102I 507 GCTCAGC GC^T[ACGT]AGC BpuAI 760 GTCTTC GAAGAC(2/6) BsiEI 2700 CGGCCG CG[GA][CT]^CG BspLU11I 1740 ACATGT A^CATGT BssHII 23 GCGCGC G^CGCGC BssSI 5497 CTCGTG CACGAG(-5/-1) BstEII 4029 GGTAACC G^GT[ACGT]ACC BstPI 4029 GGTAACC G^GT[ACGT]ACC CelII 507 GCTCAGC GC^T[ACGT]AGC DraIII 176 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2700 CGGCCG C^GGCCG EclXI 2700 CGGCCG C^GGCCG Eco52I 2700 CGGCCG C^GGCCG EcoO65I 4029 GGTAACC G^GT[ACGT]ACC FauI 3590 CCCGC CCCGC(4/6) FbaI 2054 TGATCA T^GATCA HpaI 3670 GTTAAC GTT^AAC MfeI 1439 CAATTG C^AATTG MluNI 2328 TGGCCA TGG^CCA MscI 2328 TGGCCA TGG^CCA MunI 1439 CAATTG C^AATTG NcoI 4025 CCATGG C^CATGG PflFI 3369 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 329 GAGCTC GAGCT^C SpeI 889 ACTAGT A^CTAGT SphI 3271 GCATGC GCATG^C StuI 3626 AGGCCT AGG^CCT Tth111I 3369 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 479 TCTAGA T^CTAGA XcmI 5570 CCATCTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 4112 GACGCC G[GA]^CG[CT]C 4905 GACGCC AseI 1839 ATTAAT AT^TAAT 5259 ATTAAT AsnI 1839 ATTAAT AT^TAAT 5259 ATTAAT Asp700I 1970 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2967 GAAAGCATTC BbrPI 654 CACGTG CAC^GTG 1810 CACGTG BbvCI 2236 CCTCAGC CCTCAGC(-5/-2) 3463 CCTCAGC BsaAI 654 CACGTG [CT]AC^GT[GA] 1810 CACGTG BsaHI 4112 GACGCC G[GA]^CG[CT]C 4905 GACGCC BsaWI 367 TCCGGT [AT]^CCGG[AT] 2529 ACCGGA BsgI 170 CTGCAC GTGCAG(16/14) 3425 CTGCAC Bsp1407I 1737 TGTACA T^GTACA 2723 TGTACA BsrGI 1737 TGTACA T^GTACA 2723 TGTACA BstXI 195 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3608 CCAAAGTGCTGG BstYI 3412 GGATCT [GA]^GATC[CT] 4611 AGATCT DraII 3804 AGGCCCT [GA]G^G[ACGT]CC[CT] 4649 AGGCCCT EcoO109I 3804 AGGCCCT [GA]G^G[ACGT]CC[CT] 4649 AGGCCCT Hin1I 4112 GACGCC G[GA]^CG[CT]C 4905 GACGCC HincII 1408 GTTGAC GT[CT]^[GA]AC 3670 GTTAAC HindII 1408 GTTGAC GT[CT]^[GA]AC 3670 GTTAAC MflI 3412 GGATCT [GA]^GATC[CT] 4611 AGATCT PflMI 2207 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5540 CCAGTTGGTGG PmaCI 654 CACGTG CAC^GTG 1810 CACGTG PmlI 654 CACGTG CAC^GTG 1810 CACGTG PshBI 1839 ATTAAT AT^TAAT 5259 ATTAAT ScaI 111 AGTACT AGT^ACT 1387 AGTACT SspBI 1737 TGTACA T^GTACA 2723 TGTACA Van91I 2207 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5540 CCAGTTGGTGG XhoII 3412 GGATCT [GA]^GATC[CT] 4611 AGATCT XmnI 1970 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2967 GAAAGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AflIII AluI AlwI AocI ApoI AspHI AvaI AvaII BanI BbvI BcnI BfaI BfrI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI Eco81I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI