##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI BbeI BclI BfrI
BglII BsaBI BsiWI BspDI BspHI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
FbaI FseI HincII HindII HindIII
HpaI KasI KspI MamI MluI
NarI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfoI SfuI SgrAI SnaBI
SphI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1403 GGTACC G^GTACC
AccIII 1463 TCCGGA T^CCGGA
AcyI 596 GGCGTC G[GA]^CG[CT]C
AhdI 591 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1403 GGTACC G^GTACC
AspEI 591 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1260 CACGTG CAC^GTG
BglI 1992 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1260 CACGTG [CT]AC^GT[GA]
BsaHI 596 GGCGTC G[GA]^CG[CT]C
BseAI 1463 TCCGGA T^CCGGA
BsiEI 5442 CGGTCG CG[GA][CT]^CG
Bsp1407I 4054 TGTACA T^GTACA
BspEI 1463 TCCGGA T^CCGGA
BspLU11I 1538 ACATGT A^CATGT
BsrDI 5010 CATTGC GCAATG(2/0)
BsrFI 3595 GCCGGC [GA]^CCGG[CT]
BsrGI 4054 TGTACA T^GTACA
BssHII 4086 GCGCGC G^CGCGC
Cfr10I 3595 GCCGGC [GA]^CCGG[CT]
Eam1105I 591 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2875 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3916 GAATTC G^AATTC
EcoT22I 5007 ATGCAT ATGCA^T
Hin1I 596 GGCGTC G[GA]^CG[CT]C
KpnI 1403 GGTACC GGTAC^C
MroI 1463 TCCGGA T^CCGGA
MseI 310 TTAA T^TAA
NaeI 3595 GCCGGC GCC^GGC
NgoMIV 3595 GCCGGC G^CCGGC
NsiI 5007 ATGCAT ATGCA^T
PaeR7I 5291 CTCGAG C^TCGAG
PmaCI 1260 CACGTG CAC^GTG
PmlI 1260 CACGTG CAC^GTG
SapI 510 GAAGAGC GCTCTTC(1/4)
SbfI 5035 CCTGCAGG CCTGCA^GG
ScaI 3377 AGTACT AGT^ACT
SexAI 4496 ACCTGGT A^CC[AT]GGT
SfiI 1991 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 456 ACTAGT A^CTAGT
Sse8387I 5035 CCTGCAGG CCTGCA^GG
SspBI 4054 TGTACA T^GTACA
Tru9I 310 TTAA T^TAA
XhoI 5291 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 352 GTCTAC GT^[AC][GT]AC
3122 GTAGAC
AflIII 1259 ACACGT A^C[GA][CT]GT
1538 ACATGT
Alw44I 2058 GTGCAC G^TGCAC
2911 GTGCAC
Aor51HI 668 AGCGCT AGC^GCT
4914 AGCGCT
ApaLI 2058 GTGCAC G^TGCAC
2911 GTGCAC
Asp700I 2724 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2769 GAACACCTTC
AvrII 3235 CCTAGG C^CTAGG
3251 CCTAGG
BalI 1999 TGGCCA TGG^CCA
4341 TGGCCA
BcgI 100 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2095 GCAGGAGAATCG
BlnI 3235 CCTAGG C^CTAGG
3251 CCTAGG
BsaWI 1463 TCCGGA [AT]^CCGG[AT]
5344 ACCGGA
BsmBI 2186 CGTCTC CGTCTC(1/5)
18 GAGACG
BsmI 5167 GAATGC GAATGC(1/-1)
4298 GCATTC
BssSI 2917 CACGAG CACGAG(-5/-1)
646 CTCGTG
EaeI 1999 TGGCCA [CT]^GGCC[GA]
4341 TGGCCA
Eco47III 668 AGCGCT AGC^GCT
4914 AGCGCT
EcoRV 1878 GATATC GAT^ATC
2587 GATATC
HaeII 668 AGCGCT [GA]GCGC^[CT]
4914 AGCGCT
MfeI 1883 CAATTG C^AATTG
4648 CAATTG
MluNI 1999 TGGCCA TGG^CCA
4341 TGGCCA
MscI 1999 TGGCCA TGG^CCA
4341 TGGCCA
MunI 1883 CAATTG C^AATTG
4648 CAATTG
PshAI 4540 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5078 GACTTGTGTC
SacI 632 GAGCTC GAGCT^C
3760 GAGCTC
SmaI 972 CCCGGG CCC^GGG
1048 CCCGGG
XmaCI 972 CCCGGG C^CCGGG
1048 CCCGGG
XmaI 972 CCCGGG C^CCGGG
1048 CCCGGG
XmnI 2724 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2769 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AspI AvaI AvaII AviII
BamHI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII