##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AflIII AgeI AhdI
AscI Asp718I AspEI AviII BciVI
BclI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspLU11I Bst1107I BstBI
BstZ17I ClaI Eam1105I EcoRV FbaI
FseI FspI KpnI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PvuI RcaI SapI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 4341 AACGTT AA^CGTT
AflII 4372 CTTAAG C^TTAAG
Alw44I 4470 GTGCAC G^TGCAC
ApaLI 4470 GTGCAC G^TGCAC
AseI 957 ATTAAT AT^TAAT
AsnI 957 ATTAAT AT^TAAT
AvrII 2338 CCTAGG C^CTAGG
BamHI 1138 GGATCC G^GATCC
BbrPI 148 CACGTG CAC^GTG
BcgI 5488 CGACCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4372 CTTAAG C^TTAAG
BlnI 2338 CCTAGG C^CTAGG
BlpI 73 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
BsaAI 148 CACGTG [CT]AC^GT[GA]
Bsp1407I 5257 TGTACA T^GTACA
BsrBI 76 GAGCGG CCGCTC(-3/-3)
BsrGI 5257 TGTACA T^GTACA
BssSI 5636 CTCGTG CACGAG(-5/-1)
CelII 73 GCTGAGC GC^T[ACGT]AGC
CpoI 161 CGGTCCG CG^G[AT]CCG
DraIII 4268 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 4361 GAATTC G^AATTC
EcoT22I 2637 ATGCAT ATGCA^T
HindIII 5706 AAGCTT A^AGCTT
HpaI 2262 GTTAAC GTT^AAC
KspI 4784 CCGCGG CCGC^GG
NaeI 4668 GCCGGC GCC^GGC
NgoMIV 4668 GCCGGC G^CCGGC
NsiI 2637 ATGCAT ATGCA^T
PmaCI 148 CACGTG CAC^GTG
PmlI 148 CACGTG CAC^GTG
PshAI 5098 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 957 ATTAAT AT^TAAT
Psp1406I 4341 AACGTT AA^CGTT
RsrII 161 CGGTCCG CG^G[AT]CCG
SacII 4784 CCGCGG CCGC^GG
SalI 361 GTCGAC G^TCGAC
ScaI 2209 AGTACT AGT^ACT
SspBI 5257 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 358 GACGTC GACGT^C
816 GACGTC
Aor51HI 4687 AGCGCT AGC^GCT
5139 AGCGCT
Asp700I 552 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4972 GAATCCCTTC
BbeI 101 GGCGCC GGCGC^C
4868 GGCGCC
BglII 679 AGATCT A^GATCT
828 AGATCT
BsgI 3102 GTGCAG GTGCAG(16/14)
5195 GTGCAG
BsmBI 1093 GAGACG CGTCTC(1/5)
3940 GAGACG
BsmI 3805 GAATGC GAATGC(1/-1)
556 GCATTC
BstEII 349 GGTTACC G^GT[ACGT]ACC
498 GGTCACC
BstPI 349 GGTTACC G^GT[ACGT]ACC
498 GGTCACC
DraI 1772 TTTAAA TTT^AAA
4434 TTTAAA
DrdI 4693 GACACCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5170 GACGCGGCAGTC
Eco47III 4687 AGCGCT AGC^GCT
5139 AGCGCT
EcoO65I 349 GGTTACC G^GT[ACGT]ACC
498 GGTCACC
HincII 361 GTCGAC GT[CT]^[GA]AC
2262 GTTAAC
HindII 361 GTCGAC GT[CT]^[GA]AC
2262 GTTAAC
KasI 101 GGCGCC G^GCGCC
4868 GGCGCC
NarI 101 GGCGCC GG^CGCC
4868 GGCGCC
PflMI 1976 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5551 CCAAAAGCTGG
PpuMI 1664 GGGTCCC [GA]G^G[AT]CC[CT]
4959 AGGACCC
PvuII 71 CAGCTG CAG^CTG
613 CAGCTG
SexAI 317 ACCTGGT A^CC[AT]GGT
1186 ACCTGGT
SfiI 4124 GGCCCTTAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4649 GGCCGGTGAGGCC
SfoI 101 GGCGCC GGC^GCC
4868 GGCGCC
Van91I 1976 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5551 CCAAAAGCTGG
XmnI 552 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4972 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmaCI XmaI