##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AgeI Aor51HI AscI
AviII AvrII BbrPI BcgI BglI
BlnI BsaAI BsaBI BsiEI BsiWI
BspDI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRV FseI FspI HindIII
KspI MamI MfeI MluI MunI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3663 GTCTAC GT^[AC][GT]AC
AccIII 3599 TCCGGA T^CCGGA
AclI 1983 AACGTT AA^CGTT
AcyI 3911 GGCGCC G[GA]^CG[CT]C
AflII 5130 CTTAAG C^TTAAG
AflIII 631 ACACGT A^C[GA][CT]GT
AseI 1992 ATTAAT AT^TAAT
AsnI 1992 ATTAAT AT^TAAT
BalI 2944 TGGCCA TGG^CCA
BamHI 58 GGATCC G^GATCC
BbeI 3911 GGCGCC GGCGC^C
BclI 5076 TGATCA T^GATCA
BfrI 5130 CTTAAG C^TTAAG
BsaHI 3911 GGCGCC G[GA]^CG[CT]C
BseAI 3599 TCCGGA T^CCGGA
BsgI 1000 CTGCAC GTGCAG(16/14)
BsmBI 727 GAGACG CGTCTC(1/5)
BspEI 3599 TCCGGA T^CCGGA
BspHI 1718 TCATGA T^CATGA
BspMI 998 ACCTGC ACCTGC(4/8)
BsrDI 1730 CATTGC GCAATG(2/0)
BstXI 3262 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4281 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2944 TGGCCA [CT]^GGCC[GA]
EcoNI 3211 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3316 ATGCAT ATGCA^T
FbaI 5076 TGATCA T^GATCA
HaeII 3911 GGCGCC [GA]GCGC^[CT]
HgaI 1367 GACGC GACGC(5/10)
Hin1I 3911 GGCGCC G[GA]^CG[CT]C
KasI 3911 GGCGCC G^GCGCC
MluNI 2944 TGGCCA TGG^CCA
MroI 3599 TCCGGA T^CCGGA
MscI 2944 TGGCCA TGG^CCA
NarI 3911 GGCGCC GG^CGCC
NdeI 1140 CATATG CA^TATG
NsiI 3316 ATGCAT ATGCA^T
PshBI 1992 ATTAAT AT^TAAT
Psp1406I 1983 AACGTT AA^CGTT
RcaI 1718 TCATGA T^CATGA
SfoI 3911 GGCGCC GGC^GCC
XbaI 4027 TCTAGA T^CTAGA
XcmI 264 CCAGGCGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1535 GGTACC G^GTACC
1556 GGTACC
AhdI 2865 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4872 GACTGGATGTC
Alw44I 2682 GTGCAC G^TGCAC
5235 GTGCAC
ApaLI 2682 GTGCAC G^TGCAC
5235 GTGCAC
Asp718I 1535 GGTACC G^GTACC
1556 GGTACC
AspEI 2865 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4872 GACTGGATGTC
BlpI 3695 GCTGAGC GC^T[ACGT]AGC
4231 GCTCAGC
Bpu1102I 3695 GCTGAGC GC^T[ACGT]AGC
4231 GCTCAGC
BstEII 1671 GGTCACC G^GT[ACGT]ACC
3027 GGTGACC
BstPI 1671 GGTCACC G^GT[ACGT]ACC
3027 GGTGACC
CelII 3695 GCTGAGC GC^T[ACGT]AGC
4231 GCTCAGC
CfoI 1734 GCGC GCG^C
3912 GCGC
DraI 3735 TTTAAA TTT^AAA
5189 TTTAAA
Eam1105I 2865 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4872 GACTGGATGTC
EcoO65I 1671 GGTCACC G^GT[ACGT]ACC
3027 GGTGACC
EcoRI 1913 GAATTC G^AATTC
4264 GAATTC
HhaI 1734 GCGC GCG^C
3912 GCGC
HinP1I 1734 GCGC G^CGC
3912 GCGC
HpaI 1986 GTTAAC GTT^AAC
3777 GTTAAC
KpnI 1535 GGTACC GGTAC^C
1556 GGTACC
PaeR7I 1296 CTCGAG C^TCGAG
3729 CTCGAG
SacI 1231 GAGCTC GAGCT^C
4805 GAGCTC
XhoI 1296 CTCGAG C^TCGAG
3729 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrBI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DrdI DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII XmnI