##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI AscI AspEI AviII BcgI
BciVI BfrI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI BstUI ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI FspI HgaI KspI MfeI
MluI MroI MunI MvnI NaeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshAI Psp1406I
RsrII SacII SalI SapI SfiI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 308 GACGTC GACGT^C
Aor51HI 5489 AGCGCT AGC^GCT
AseI 2801 ATTAAT AT^TAAT
AsnI 2801 ATTAAT AT^TAAT
Asp700I 788 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3215 CCTAGG C^CTAGG
BbeI 1148 GGCGCC GGCGC^C
BbrPI 1235 CACGTG CAC^GTG
BbvCI 4659 CCTCAGC CCTCAGC(-5/-2)
BclI 4320 TGATCA T^GATCA
BlnI 3215 CCTAGG C^CTAGG
BsaBI 817 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2344 CGATCG CG[GA][CT]^CG
BsmI 3232 GCATTC GAATGC(1/-1)
Bst1107I 667 GTATAC GTA^TAC
BstBI 1809 TTCGAA TT^CGAA
BstZ17I 667 GTATAC GTA^TAC
DraI 4443 TTTAAA TTT^AAA
DrdI 1870 GACGAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5489 AGCGCT AGC^GCT
EcoRI 2065 GAATTC G^AATTC
FbaI 4320 TGATCA T^GATCA
HpaI 1690 GTTAAC GTT^AAC
KasI 1148 GGCGCC G^GCGCC
MamI 817 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1148 GGCGCC GG^CGCC
NheI 1781 GCTAGC G^CTAGC
NspV 1809 TTCGAA TT^CGAA
PflMI 2160 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1235 CACGTG CAC^GTG
PmlI 1235 CACGTG CAC^GTG
PshBI 2801 ATTAAT AT^TAAT
PvuI 2344 CGATCG CGAT^CG
SbfI 4003 CCTGCAGG CCTGCA^GG
ScaI 3814 AGTACT AGT^ACT
SexAI 578 ACCTGGT A^CC[AT]GGT
SfoI 1148 GGCGCC GGC^GCC
SfuI 1809 TTCGAA TT^CGAA
Sse8387I 4003 CCTGCAGG CCTGCA^GG
StuI 3835 AGGCCT AGG^CCT
Van91I 2160 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5256 TCTAGA T^CTAGA
XmnI 788 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 582 GGTACC G^GTACC
1459 GGTACC
AcyI 308 GACGTC G[GA]^CG[CT]C
1148 GGCGCC
Alw44I 1238 GTGCAC G^TGCAC
2618 GTGCAC
AocI 1243 CCTGAGG CC^T[ACGT]AGG
4362 CCTAAGG
ApaI 659 GGGCCC GGGCC^C
1132 GGGCCC
ApaLI 1238 GTGCAC G^TGCAC
2618 GTGCAC
Asp718I 582 GGTACC G^GTACC
1459 GGTACC
AspI 2702 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5213 GACCTAGTC
BamHI 799 GGATCC G^GATCC
858 GGATCC
BglII 1119 AGATCT A^GATCT
1597 AGATCT
BsaHI 308 GACGTC G[GA]^CG[CT]C
1148 GGCGCC
BsmBI 306 GAGACG CGTCTC(1/5)
4734 GAGACG
BspLU11I 4470 ACATGT A^CATGT
5336 ACATGT
BsrBI 4166 CCGCTC CCGCTC(-3/-3)
674 GAGCGG
BsrDI 159 GCAATG GCAATG(2/0)
3426 GCAATG
BsrFI 1727 GCCGGT [GA]^CCGG[CT]
4081 GCCGGT
BstAPI 150 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
289 GCAGCGGGTGC
BstEII 137 GGTTACC G^GT[ACGT]ACC
5210 GGTGACC
BstPI 137 GGTTACC G^GT[ACGT]ACC
5210 GGTGACC
Bsu36I 1243 CCTGAGG CC^T[ACGT]AGG
4362 CCTAAGG
Cfr10I 1727 GCCGGT [GA]^CCGG[CT]
4081 GCCGGT
DraIII 3237 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3937 CACTGGGTG
Eco81I 1243 CCTGAGG CC^T[ACGT]AGG
4362 CCTAAGG
EcoO65I 137 GGTTACC G^GT[ACGT]ACC
5210 GGTGACC
HaeII 1148 GGCGCC [GA]GCGC^[CT]
5489 AGCGCT
Hin1I 308 GACGTC G[GA]^CG[CT]C
1148 GGCGCC
KpnI 582 GGTACC GGTAC^C
1459 GGTACC
MslI 1013 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1554 CATATGAGTG
NcoI 1471 CCATGG C^CATGG
2779 CCATGG
NdeI 45 CATATG CA^TATG
1554 CATATG
PflFI 2702 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5213 GACCTAGTC
SacI 1076 GAGCTC GAGCT^C
2087 GAGCTC
SmaI 4586 CCCGGG CCC^GGG
4636 CCCGGG
Tth111I 2702 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5213 GACCTAGTC
XmaCI 4586 CCCGGG C^CCGGG
4636 CCCGGG
XmaI 4586 CCCGGG C^CCGGG
4636 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII