##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BcgI BfrI BglII BsaBI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DrdI
Eco47III EcoRI EcoRV FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI Tth111I Van91I XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2320 GACGTC GACGT^C
AcsI 2423 GAATTT [GA]^AATT[CT]
AhdI 1274 GACGCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2423 GAATTT [GA]^AATT[CT]
AspEI 1274 GACGCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3781 GGCGCC GGCGC^C
BbrPI 1938 CACGTG CAC^GTG
BciVI 1449 GGATAC GTATCC(6/5)
BclI 5300 TGATCA T^GATCA
BsaAI 1938 CACGTG [CT]AC^GT[GA]
BstXI 1138 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 1274 GACGCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 131 ATGCAT ATGCA^T
FbaI 5300 TGATCA T^GATCA
KasI 3781 GGCGCC G^GCGCC
NarI 3781 GGCGCC GG^CGCC
NsiI 131 ATGCAT ATGCA^T
PaeR7I 1847 CTCGAG C^TCGAG
PmaCI 1938 CACGTG CAC^GTG
PmlI 1938 CACGTG CAC^GTG
SalI 4100 GTCGAC G^TCGAC
SapI 4974 GCTCTTC GCTCTTC(1/4)
SbfI 711 CCTGCAGG CCTGCA^GG
SfoI 3781 GGCGCC GGC^GCC
SmaI 4712 CCCGGG CCC^GGG
Sse8387I 711 CCTGCAGG CCTGCA^GG
SwaI 2471 ATTTAAAT ATTT^AAAT
XhoI 1847 CTCGAG C^TCGAG
XmaCI 4712 CCCGGG C^CCGGG
XmaI 4712 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 2320 GACGTC G[GA]^CG[CT]C
3781 GGCGCC
Alw44I 2611 GTGCAC G^TGCAC
3469 GTGCAC
ApaLI 2611 GTGCAC G^TGCAC
3469 GTGCAC
BalI 4797 TGGCCA TGG^CCA
5065 TGGCCA
BbsI 327 GTCTTC GAAGAC(2/6)
2532 GTCTTC
BpuAI 327 GTCTTC GAAGAC(2/6)
2532 GTCTTC
BsaHI 2320 GACGTC G[GA]^CG[CT]C
3781 GGCGCC
BsiEI 3094 CGGCCG CG[GA][CT]^CG
3159 CGGCCG
BsmI 549 GAATGC GAATGC(1/-1)
1387 GAATGC
BstEII 2370 GGTCACC G^GT[ACGT]ACC
5190 GGTGACC
BstPI 2370 GGTCACC G^GT[ACGT]ACC
5190 GGTGACC
DraI 2451 TTTAAA TTT^AAA
2472 TTTAAA
EagI 3094 CGGCCG C^GGCCG
3159 CGGCCG
EclXI 3094 CGGCCG C^GGCCG
3159 CGGCCG
Eco52I 3094 CGGCCG C^GGCCG
3159 CGGCCG
EcoO65I 2370 GGTCACC G^GT[ACGT]ACC
5190 GGTGACC
Hin1I 2320 GACGTC G[GA]^CG[CT]C
3781 GGCGCC
MluNI 4797 TGGCCA TGG^CCA
5065 TGGCCA
MscI 4797 TGGCCA TGG^CCA
5065 TGGCCA
SphI 11 GCATGC GCATG^C
3805 GCATGC
TaqI 1848 TCGA T^CGA
4101 TCGA
TthHB8I 1848 TCGA T^CGA
4101 TCGA
XbaI 545 TCTAGA T^CTAGA
4016 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII AvrII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII