##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BclI BfrI BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BsrFI BssHII BstAPI BstUI
CelII Cfr10I ClaI CpoI DraIII
DrdI Eam1105I Eco47III FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MluI MroI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 5326 GGCGCC G[GA]^CG[CT]C
Alw44I 4746 GTGCAC G^TGCAC
AlwNI 1248 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 5237 CCTGAGG CC^T[ACGT]AGG
ApaLI 4746 GTGCAC G^TGCAC
BanI 5326 GGCGCC G^G[CT][GA]CC
BbeI 5326 GGCGCC GGCGC^C
BcgI 4118 GCAGATAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 5220 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 987 CACGTA [CT]AC^GT[GA]
BsaHI 5326 GGCGCC G[GA]^CG[CT]C
BsiEI 5174 CGGCCG CG[GA][CT]^CG
BsmI 3673 GAATGC GAATGC(1/-1)
BspHI 388 TCATGA T^CATGA
Bst1107I 2623 GTATAC GTA^TAC
BstBI 845 TTCGAA TT^CGAA
BstEII 2418 GGTCACC G^GT[ACGT]ACC
BstPI 2418 GGTCACC G^GT[ACGT]ACC
BstZ17I 2623 GTATAC GTA^TAC
Bsu36I 5237 CCTGAGG CC^T[ACGT]AGG
EagI 5174 CGGCCG C^GGCCG
EclXI 5174 CGGCCG C^GGCCG
Eco52I 5174 CGGCCG C^GGCCG
Eco81I 5237 CCTGAGG CC^T[ACGT]AGG
EcoNI 3802 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2418 GGTCACC G^GT[ACGT]ACC
EcoRI 3331 GAATTC G^AATTC
EcoT14I 4218 CCAAGG C^C[AT][AT]GG
FauI 5386 GCGGG CCCGC(4/6)
HaeII 5326 GGCGCC [GA]GCGC^[CT]
Hin1I 5326 GGCGCC G[GA]^CG[CT]C
KasI 5326 GGCGCC G^GCGCC
MaeII 988 ACGT A^CGT
NarI 5326 GGCGCC GG^CGCC
NspV 845 TTCGAA TT^CGAA
PaeR7I 1499 CTCGAG C^TCGAG
RcaI 388 TCATGA T^CATGA
SacI 1189 GAGCTC GAGCT^C
SfoI 5326 GGCGCC GGC^GCC
SfuI 845 TTCGAA TT^CGAA
SmaI 5378 CCCGGG CCC^GGG
StyI 4218 CCAAGG C^C[AT][AT]GG
XhoI 1499 CTCGAG C^TCGAG
XmaCI 5378 CCCGGG C^CCGGG
XmaI 5378 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 1499 CTCGAG C^[CT]CG[GA]G
5378 CCCGGG
BalI 4182 TGGCCA TGG^CCA
5264 TGGCCA
BbvCI 3767 CCTCAGC CCTCAGC(-5/-2)
5354 GCTGAGG
BpmI 5422 CTCCAG CTGGAG(16/14)
615 CTGGAG
BsgI 4570 CTGCAC GTGCAG(16/14)
1319 GTGCAG
BsmBI 5284 CGTCTC CGTCTC(1/5)
5451 CGTCTC
BsoBI 1499 CTCGAG C^[CT]CG[GA]G
5378 CCCGGG
BspMI 955 ACCTGC ACCTGC(4/8)
3808 GCAGGT
BssSI 2949 CACGAG CACGAG(-5/-1)
4969 CACGAG
BstXI 1597 CCAGATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5205 CCAGCACTTTGG
CfoI 4116 GCGC GCG^C
5327 GCGC
DraII 2738 AGGTCCC [GA]G^G[ACGT]CC[CT]
2834 AGGTCCC
DsaI 3226 CCGTGG C^C[GA][CT]GG
3247 CCGTGG
EcoO109I 2738 AGGTCCC [GA]G^G[ACGT]CC[CT]
2834 AGGTCCC
EcoT22I 876 ATGCAT ATGCA^T
4775 ATGCAT
HhaI 4116 GCGC GCG^C
5327 GCGC
HinP1I 4116 GCGC G^CGC
5327 GCGC
HincII 3881 GTCAAC GT[CT]^[GA]AC
5165 GTTGAC
HindII 3881 GTCAAC GT[CT]^[GA]AC
5165 GTTGAC
MluNI 4182 TGGCCA TGG^CCA
5264 TGGCCA
MscI 4182 TGGCCA TGG^CCA
5264 TGGCCA
NsiI 876 ATGCAT ATGCA^T
4775 ATGCAT
PpuMI 2738 AGGTCCC [GA]G^G[AT]CC[CT]
2834 AGGTCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpuAI
BsaBI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoRV Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI