##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII BclI BfrI
BsiWI BspHI Bst1107I BstBI BstZ17I
CpoI Eam1105I EcoRI FbaI FseI
FspI KpnI MluI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 4611 ACATGT A^C[GA][CT]GT
AgeI 580 ACCGGT A^CCGGT
Aor51HI 1392 AGCGCT AGC^GCT
BamHI 1376 GGATCC G^GATCC
BbrPI 4953 CACGTG CAC^GTG
BbsI 975 GAAGAC GAAGAC(2/6)
BciVI 362 GTATCC GTATCC(6/5)
BglII 1952 AGATCT A^GATCT
BpuAI 975 GAAGAC GAAGAC(2/6)
BsaI 134 GAGACC GGTCTC(1/5)
BsmBI 3165 CGTCTC CGTCTC(1/5)
Bsp1407I 4601 TGTACA T^GTACA
BspDI 4383 ATCGAT AT^CGAT
BspLU11I 4611 ACATGT A^CATGT
BsrDI 770 GCAATG GCAATG(2/0)
BsrGI 4601 TGTACA T^GTACA
BssHII 837 GCGCGC G^CGCGC
BstEII 2127 GGTGACC G^GT[ACGT]ACC
BstPI 2127 GGTGACC G^GT[ACGT]ACC
ClaI 4383 ATCGAT AT^CGAT
DraI 4773 TTTAAA TTT^AAA
DraIII 3248 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3981 GACCAGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1392 AGCGCT AGC^GCT
EcoO65I 2127 GGTGACC G^GT[ACGT]ACC
EcoRV 274 GATATC GAT^ATC
EcoT22I 4763 ATGCAT ATGCA^T
HindIII 2752 AAGCTT A^AGCTT
HpaI 4701 GTTAAC GTT^AAC
KspI 4282 CCGCGG CCGC^GG
MfeI 4730 CAATTG C^AATTG
MunI 4730 CAATTG C^AATTG
NsiI 4763 ATGCAT ATGCA^T
PinAI 580 ACCGGT A^CCGGT
PmaCI 4953 CACGTG CAC^GTG
PmlI 4953 CACGTG CAC^GTG
SacII 4282 CCGCGG CCGC^GG
SapI 4020 GAAGAGC GCTCTTC(1/4)
SexAI 1868 ACCAGGT A^CC[AT]GGT
SmaI 1545 CCCGGG CCC^GGG
SnaBI 1284 TACGTA TAC^GTA
SphI 776 GCATGC GCATG^C
SspBI 4601 TGTACA T^GTACA
StuI 1367 AGGCCT AGG^CCT
XmaCI 1545 CCCGGG C^CCGGG
XmaI 1545 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1000 GAATTT [GA]^AATT[CT]
4781 GAATTT
Alw44I 141 GTGCAC G^TGCAC
2234 GTGCAC
ApaLI 141 GTGCAC G^TGCAC
2234 GTGCAC
ApoI 1000 GAATTT [GA]^AATT[CT]
4781 GAATTT
Asp700I 1314 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4781 GAATTTATTC
AvrII 2997 CCTAGG C^CTAGG
4215 CCTAGG
BcgI 1419 CGATCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4091 GCACCATGTTCG
BlnI 2997 CCTAGG C^CTAGG
4215 CCTAGG
BsaAI 1284 TACGTA [CT]AC^GT[GA]
4953 CACGTG
BsmI 169 GAATGC GAATGC(1/-1)
4517 GAATGC
BssSI 1207 CACGAG CACGAG(-5/-1)
1504 CACGAG
BstAPI 1353 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4640 GCAGCTCCTGC
BstXI 1812 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2288 CCATGGGGCTGG
NheI 3885 GCTAGC G^CTAGC
4676 GCTAGC
NspI 776 GCATGC [GA]CATG^[CT]
4611 ACATGT
XmnI 1314 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4781 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII