##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AclI AgeI Aor51HI
AscI AseI AsnI AspI AviII
BsaBI BsiEI BsiWI BsmBI BsmI
BspDI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Cfr10I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRI FauI FseI
FspI HgaI KspI MamI MfeI
MluI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3787 GGTACC G^GTACC
AccI 4178 GTCTAC GT^[AC][GT]AC
AccIII 1084 TCCGGA T^CCGGA
AcsI 455 AAATTT [GA]^AATT[CT]
AcyI 4978 GGCGCC G[GA]^CG[CT]C
AflII 4020 CTTAAG C^TTAAG
ApoI 455 AAATTT [GA]^AATT[CT]
Asp718I 3787 GGTACC G^GTACC
AvrII 4624 CCTAGG C^CTAGG
BalI 3632 TGGCCA TGG^CCA
BbeI 4978 GGCGCC GGCGC^C
BbrPI 2711 CACGTG CAC^GTG
BbsI 4189 GTCTTC GAAGAC(2/6)
BcgI 3212 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1285 GTATCC GTATCC(6/5)
BfrI 4020 CTTAAG C^TTAAG
BglI 4451 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4624 CCTAGG C^CTAGG
BpuAI 4189 GTCTTC GAAGAC(2/6)
BsaHI 4978 GGCGCC G[GA]^CG[CT]C
BseAI 1084 TCCGGA T^CCGGA
BspEI 1084 TCCGGA T^CCGGA
BspHI 2564 TCATGA T^CATGA
BspLU11I 746 ACATGT A^CATGT
BstAPI 2854 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1474 GGTCACC G^GT[ACGT]ACC
BstPI 1474 GGTCACC G^GT[ACGT]ACC
DrdI 3366 GACATGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3632 TGGCCA [CT]^GGCC[GA]
EcoO65I 1474 GGTCACC G^GT[ACGT]ACC
EcoRV 1188 GATATC GAT^ATC
EcoT22I 3554 ATGCAT ATGCA^T
Hin1I 4978 GGCGCC G[GA]^CG[CT]C
HincII 367 GTTAAC GT[CT]^[GA]AC
HindII 367 GTTAAC GT[CT]^[GA]AC
HpaI 367 GTTAAC GTT^AAC
KasI 4978 GGCGCC G^GCGCC
KpnI 3787 GGTACC GGTAC^C
MluNI 3632 TGGCCA TGG^CCA
MroI 1084 TCCGGA T^CCGGA
MscI 3632 TGGCCA TGG^CCA
NarI 4978 GGCGCC GG^CGCC
NsiI 3554 ATGCAT ATGCA^T
PmaCI 2711 CACGTG CAC^GTG
PmlI 2711 CACGTG CAC^GTG
RcaI 2564 TCATGA T^CATGA
SfiI 4450 GGCCTGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4978 GGCGCC GGC^GCC
SmaI 3575 CCCGGG CCC^GGG
XcmI 846 CCAGCCTCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3575 CCCGGG C^CCGGG
XmaI 3575 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 746 ACATGT A^C[GA][CT]GT
5103 ACGTGT
Asp700I 643 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2037 GAAGGGCTTC
AvaI 3575 CCCGGG C^[CT]CG[GA]G
4934 CCCGAG
BamHI 2928 GGATCC G^GATCC
3890 GGATCC
BclI 2062 TGATCA T^GATCA
4907 TGATCA
BglII 241 AGATCT A^GATCT
4197 AGATCT
BsaAI 2711 CACGTG [CT]AC^GT[GA]
3922 CACGTA
BsoBI 3575 CCCGGG C^[CT]CG[GA]G
4934 CCCGAG
BspMI 182 ACCTGC ACCTGC(4/8)
2753 GCAGGT
DsaI 1029 CCATGG C^C[GA][CT]GG
2738 CCATGG
EcoNI 1315 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2770 CCTCTGACAGG
FbaI 2062 TGATCA T^GATCA
4907 TGATCA
HindIII 2638 AAGCTT A^AGCTT
3938 AAGCTT
NcoI 1029 CCATGG C^CATGG
2738 CCATGG
PshAI 4239 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4551 GACAGGAGTC
SapI 4065 GAAGAGC GCTCTTC(1/4)
3725 GCTCTTC
SphI 1037 GCATGC GCATG^C
3177 GCATGC
XmnI 643 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2037 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AfaI AhdI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspEI AspHI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII Eam1105I EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII