##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI ApaI AscI Asp718I
AspEI AvrII BamHI BanII BcnI
BglI BlnI BsaBI BsaI BseAI
BsiWI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FseI
HindIII KpnI KspI MamI MluI
MroI NaeI NciI NgoMIV NheI
NotI NspV PacI PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Alw44I 3016 GTGCAC G^TGCAC
Aor51HI 1659 AGCGCT AGC^GCT
ApaLI 3016 GTGCAC G^TGCAC
AseI 3999 ATTAAT AT^TAAT
AsnI 3999 ATTAAT AT^TAAT
AspI 2182 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5298 TGCGCA TGC^GCA
BalI 4619 TGGCCA TGG^CCA
BanI 19 GGCGCC G^G[CT][GA]CC
BbeI 19 GGCGCC GGCGC^C
BbrPI 1765 CACGTG CAC^GTG
BlpI 1382 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1382 GCTCAGC GC^T[ACGT]AGC
BsaAI 1765 CACGTG [CT]AC^GT[GA]
BsiEI 160 CGACCG CG[GA][CT]^CG
BsmI 733 GCATTC GAATGC(1/-1)
BspHI 1630 TCATGA T^CATGA
BsrBI 4612 CCGCTC CCGCTC(-3/-3)
BsrDI 4378 GCAATG GCAATG(2/0)
CelII 1382 GCTCAGC GC^T[ACGT]AGC
DraII 1185 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 3743 GACATCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2301 CCATGG C^C[GA][CT]GG
Eco47III 1659 AGCGCT AGC^GCT
EcoO109I 1185 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1719 ATGCAT ATGCA^T
FauI 5280 GCGGG CCCGC(4/6)
FspI 5298 TGCGCA TGC^GCA
KasI 19 GGCGCC G^GCGCC
MluNI 4619 TGGCCA TGG^CCA
MscI 4619 TGGCCA TGG^CCA
NarI 19 GGCGCC GG^CGCC
NcoI 2301 CCATGG C^CATGG
NsiI 1719 ATGCAT ATGCA^T
PaeR7I 11 CTCGAG C^TCGAG
PflFI 2182 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1682 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1765 CACGTG CAC^GTG
PmlI 1765 CACGTG CAC^GTG
PpuMI 1185 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1575 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3999 ATTAAT AT^TAAT
PvuII 1287 CAGCTG CAG^CTG
RcaI 1630 TCATGA T^CATGA
SexAI 1687 ACCTGGT A^CC[AT]GGT
SfoI 19 GGCGCC GGC^GCC
SphI 4468 GCATGC GCATG^C
StuI 117 AGGCCT AGG^CCT
Tth111I 2182 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1682 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 858 CCATCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 11 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2139 GTAGAC GT^[AC][GT]AC
4215 GTCTAC
AcyI 19 GGCGCC G[GA]^CG[CT]C
254 GACGCC
AvaI 11 CTCGAG C^[CT]CG[GA]G
23 CCCGAG
BbvCI 4438 CCTCAGC CCTCAGC(-5/-2)
2350 GCTGAGG
BcgI 4427 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
91 GCAGACTGGTCG
BglII 293 AGATCT A^GATCT
1937 AGATCT
BpmI 1557 CTCCAG CTGGAG(16/14)
4371 CTGGAG
BsaHI 19 GGCGCC G[GA]^CG[CT]C
254 GACGCC
BsaWI 162 ACCGGA [AT]^CCGG[AT]
5303 ACCGGA
BsmBI 1362 CGTCTC CGTCTC(1/5)
252 GAGACG
BsoBI 11 CTCGAG C^[CT]CG[GA]G
23 CCCGAG
EaeI 57 TGGCCG [CT]^GGCC[GA]
4619 TGGCCA
EcoNI 10 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4711 CCTTAACTAGG
EcoRI 1747 GAATTC G^AATTC
2038 GAATTC
HgaI 254 GACGC GACGC(5/10)
1442 GCGTC
Hin1I 19 GGCGCC G[GA]^CG[CT]C
254 GACGCC
HpaI 3867 GTTAAC GTT^AAC
4035 GTTAAC
MfeI 556 CAATTG C^AATTG
1205 CAATTG
MspA1I 1134 CAGCGG C[AC]G^C[GT]G
1287 CAGCTG
MunI 556 CAATTG C^AATTG
1205 CAATTG
NdeI 1458 CATATG CA^TATG
2520 CATATG
NruI 65 TCGCGA TCG^CGA
100 TCGCGA
SapI 428 GCTCTTC GCTCTTC(1/4)
531 GCTCTTC
SpeI 701 ACTAGT A^CTAGT
4055 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BbsI BbvI
BciVI BclI BfaI BfrI BmyI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI