################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI AocI ApaI AscI Asp718I AspEI AvrII BamHI BanII BcnI BglI BlnI BsaBI BsaI BseAI BsiWI BspDI BspEI BspMI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FseI HindIII KpnI KspI MamI MluI MroI NaeI NciI NgoMIV NheI NotI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Alw44I 3016 GTGCAC G^TGCAC Aor51HI 1659 AGCGCT AGC^GCT ApaLI 3016 GTGCAC G^TGCAC AseI 3999 ATTAAT AT^TAAT AsnI 3999 ATTAAT AT^TAAT AspI 2182 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5298 TGCGCA TGC^GCA BalI 4619 TGGCCA TGG^CCA BanI 19 GGCGCC G^G[CT][GA]CC BbeI 19 GGCGCC GGCGC^C BbrPI 1765 CACGTG CAC^GTG BlpI 1382 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1382 GCTCAGC GC^T[ACGT]AGC BsaAI 1765 CACGTG [CT]AC^GT[GA] BsiEI 160 CGACCG CG[GA][CT]^CG BsmI 733 GCATTC GAATGC(1/-1) BspHI 1630 TCATGA T^CATGA BsrBI 4612 CCGCTC CCGCTC(-3/-3) BsrDI 4378 GCAATG GCAATG(2/0) CelII 1382 GCTCAGC GC^T[ACGT]AGC DraII 1185 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 3743 GACATCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2301 CCATGG C^C[GA][CT]GG Eco47III 1659 AGCGCT AGC^GCT EcoO109I 1185 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 1719 ATGCAT ATGCA^T FauI 5280 GCGGG CCCGC(4/6) FspI 5298 TGCGCA TGC^GCA KasI 19 GGCGCC G^GCGCC MluNI 4619 TGGCCA TGG^CCA MscI 4619 TGGCCA TGG^CCA NarI 19 GGCGCC GG^CGCC NcoI 2301 CCATGG C^CATGG NsiI 1719 ATGCAT ATGCA^T PaeR7I 11 CTCGAG C^TCGAG PflFI 2182 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1682 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1765 CACGTG CAC^GTG PmlI 1765 CACGTG CAC^GTG PpuMI 1185 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1575 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3999 ATTAAT AT^TAAT PvuII 1287 CAGCTG CAG^CTG RcaI 1630 TCATGA T^CATGA SexAI 1687 ACCTGGT A^CC[AT]GGT SfoI 19 GGCGCC GGC^GCC SphI 4468 GCATGC GCATG^C StuI 117 AGGCCT AGG^CCT Tth111I 2182 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1682 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 858 CCATCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 11 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 2139 GTAGAC GT^[AC][GT]AC 4215 GTCTAC AcyI 19 GGCGCC G[GA]^CG[CT]C 254 GACGCC AvaI 11 CTCGAG C^[CT]CG[GA]G 23 CCCGAG BbvCI 4438 CCTCAGC CCTCAGC(-5/-2) 2350 GCTGAGG BcgI 4427 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 91 GCAGACTGGTCG BglII 293 AGATCT A^GATCT 1937 AGATCT BpmI 1557 CTCCAG CTGGAG(16/14) 4371 CTGGAG BsaHI 19 GGCGCC G[GA]^CG[CT]C 254 GACGCC BsaWI 162 ACCGGA [AT]^CCGG[AT] 5303 ACCGGA BsmBI 1362 CGTCTC CGTCTC(1/5) 252 GAGACG BsoBI 11 CTCGAG C^[CT]CG[GA]G 23 CCCGAG EaeI 57 TGGCCG [CT]^GGCC[GA] 4619 TGGCCA EcoNI 10 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4711 CCTTAACTAGG EcoRI 1747 GAATTC G^AATTC 2038 GAATTC HgaI 254 GACGC GACGC(5/10) 1442 GCGTC Hin1I 19 GGCGCC G[GA]^CG[CT]C 254 GACGCC HpaI 3867 GTTAAC GTT^AAC 4035 GTTAAC MfeI 556 CAATTG C^AATTG 1205 CAATTG MspA1I 1134 CAGCGG C[AC]G^C[GT]G 1287 CAGCTG MunI 556 CAATTG C^AATTG 1205 CAATTG NdeI 1458 CATATG CA^TATG 2520 CATATG NruI 65 TCGCGA TCG^CGA 100 TCGCGA SapI 428 GCTCTTC GCTCTTC(1/4) 531 GCTCTTC SpeI 701 ACTAGT A^CTAGT 4055 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BbsI BbvI BciVI BclI BfaI BfrI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI