##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AflII AocI AscI AvrII
BamHI BbeI BbrPI BfrI BlnI
BsaBI BsiWI BspLU11I Bst1107I BstBI
BstZ17I Bsu36I CpoI EagI EclXI
Eco52I Eco81I EcoRV FseI HpaI
KasI KspI MamI MfeI MunI
NarI NheI NotI NruI NspV
PacI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4698 GTAGAC GT^[AC][GT]AC
AhdI 3035 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 378 AGCGCT AGC^GCT
ApaI 1215 GGGCCC GGGCC^C
AseI 3224 ATTAAT AT^TAAT
AsnI 3224 ATTAAT AT^TAAT
Asp700I 1060 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3035 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1262 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1137 TGCGCA TGC^GCA
BalI 762 TGGCCA TGG^CCA
BciVI 2066 GGATAC GTATCC(6/5)
BclI 4586 TGATCA T^GATCA
BglI 4451 GCCTCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2006 TACGTG [CT]AC^GT[GA]
BsiEI 224 CGGTCG CG[GA][CT]^CG
BssHII 1009 GCGCGC G^CGCGC
BstAPI 2738 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 3035 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 378 AGCGCT AGC^GCT
EcoT22I 3396 ATGCAT ATGCA^T
FbaI 4586 TGATCA T^GATCA
FspI 1137 TGCGCA TGC^GCA
MluI 2883 ACGCGT A^CGCGT
MluNI 762 TGGCCA TGG^CCA
MscI 762 TGGCCA TGG^CCA
NaeI 2444 GCCGGC GCC^GGC
NcoI 1230 CCATGG C^CATGG
NdeI 3899 CATATG CA^TATG
NgoMIV 2444 GCCGGC G^CCGGC
NsiI 3396 ATGCAT ATGCA^T
PaeR7I 3074 CTCGAG C^TCGAG
PflFI 1262 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3224 ATTAAT AT^TAAT
ScaI 3474 AGTACT AGT^ACT
Tth111I 1262 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3074 CTCGAG C^TCGAG
XmnI 1060 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 2986 TCCGGA T^CCGGA
4028 TCCGGA
AgeI 1378 ACCGGT A^CCGGT
1616 ACCGGT
AlwNI 277 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
341 CAGTCCCTG
BglII 3882 AGATCT A^GATCT
4844 AGATCT
BlpI 3185 GCTGAGC GC^T[ACGT]AGC
4231 GCTCAGC
Bpu1102I 3185 GCTGAGC GC^T[ACGT]AGC
4231 GCTCAGC
BseAI 2986 TCCGGA T^CCGGA
4028 TCCGGA
BsmI 4520 GAATGC GAATGC(1/-1)
3398 GCATTC
Bsp1407I 1128 TGTACA T^GTACA
4377 TGTACA
BspDI 877 ATCGAT AT^CGAT
3306 ATCGAT
BspEI 2986 TCCGGA T^CCGGA
4028 TCCGGA
BspHI 3349 TCATGA T^CATGA
3828 TCATGA
BsrGI 1128 TGTACA T^GTACA
4377 TGTACA
BssSI 2345 CACGAG CACGAG(-5/-1)
4691 CACGAG
BstEII 928 GGTCACC G^GT[ACGT]ACC
4132 GGTGACC
BstPI 928 GGTCACC G^GT[ACGT]ACC
4132 GGTGACC
BstXI 615 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2209 CCATCCTTATGG
CelII 3185 GCTGAGC GC^T[ACGT]AGC
4231 GCTCAGC
ClaI 877 ATCGAT AT^CGAT
3306 ATCGAT
DraIII 2896 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4592 CACATTGTG
EcoNI 933 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1105 CCTGCACAAGG
EcoO65I 928 GGTCACC G^GT[ACGT]ACC
4132 GGTGACC
EcoRI 23 GAATTC G^AATTC
4058 GAATTC
HaeII 378 AGCGCT [GA]GCGC^[CT]
1172 AGCGCC
HincII 1748 GTCAAC GT[CT]^[GA]AC
2435 GTCAAC
HindII 1748 GTCAAC GT[CT]^[GA]AC
2435 GTCAAC
HindIII 4035 AAGCTT A^AGCTT
4902 AAGCTT
MroI 2986 TCCGGA T^CCGGA
4028 TCCGGA
NspI 4794 GCATGT [GA]CATG^[CT]
4885 GCATGT
PinAI 1378 ACCGGT A^CCGGT
1616 ACCGGT
RcaI 3349 TCATGA T^CATGA
3828 TCATGA
SacI 3071 GAGCTC GAGCT^C
3623 GAGCTC
SapI 512 GAAGAGC GCTCTTC(1/4)
3068 GAAGAGC
SexAI 2897 ACCTGGT A^CC[AT]GGT
4894 ACCTGGT
SgrAI 1377 CACCGGTG C[GA]^CCGG[CT]G
1615 CACCGGTG
SspBI 1128 TGTACA T^GTACA
4377 TGTACA
SspI 137 AATATT AAT^ATT
4626 AATATT
StuI 1083 AGGCCT AGG^CCT
2490 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AatII Acc65I AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI ApaLI ApoI Asp718I AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII