##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AocI AscI BbrPI BciVI BfrI
BsaBI BseAI BspDI BspEI BspHI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraIII DrdI
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KspI
MamI MluI MroI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcsI 515 AAATTC [GA]^AATT[CT]
AcyI 343 GGCGCC G[GA]^CG[CT]C
AgeI 3718 ACCGGT A^CCGGT
Alw44I 2753 GTGCAC G^TGCAC
Aor51HI 5021 AGCGCT AGC^GCT
ApaLI 2753 GTGCAC G^TGCAC
ApoI 515 AAATTC [GA]^AATT[CT]
AseI 5285 ATTAAT AT^TAAT
AsnI 5285 ATTAAT AT^TAAT
AspI 2716 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1721 CCTAGG C^CTAGG
BamHI 2976 GGATCC G^GATCC
BbeI 343 GGCGCC GGCGC^C
BglII 3215 AGATCT A^GATCT
BlnI 1721 CCTAGG C^CTAGG
BsaAI 933 CACGTA [CT]AC^GT[GA]
BsaHI 343 GGCGCC G[GA]^CG[CT]C
BsiEI 380 CGGCCG CG[GA][CT]^CG
BsiWI 3121 CGTACG C^GTACG
BsmBI 2721 CGTCTC CGTCTC(1/5)
Bsp1407I 5362 TGTACA T^GTACA
BsrGI 5362 TGTACA T^GTACA
BssHII 406 GCGCGC G^CGCGC
BstAPI 5239 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 5614 TTTAAA TTT^AAA
EagI 380 CGGCCG C^GGCCG
EclXI 380 CGGCCG C^GGCCG
Eco47III 5021 AGCGCT AGC^GCT
Eco52I 380 CGGCCG C^GGCCG
Hin1I 343 GGCGCC G[GA]^CG[CT]C
KasI 343 GGCGCC G^GCGCC
MfeI 953 CAATTG C^AATTG
MunI 953 CAATTG C^AATTG
NaeI 181 GCCGGC GCC^GGC
NarI 343 GGCGCC GG^CGCC
NcoI 2987 CCATGG C^CATGG
NdeI 4866 CATATG CA^TATG
NgoMIV 181 GCCGGC G^CCGGC
PaeR7I 4019 CTCGAG C^TCGAG
PflFI 2716 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3718 ACCGGT A^CCGGT
PshAI 3169 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5285 ATTAAT AT^TAAT
PstI 2833 CTGCAG CTGCA^G
SapI 308 GAAGAGC GCTCTTC(1/4)
ScaI 1275 AGTACT AGT^ACT
SfoI 343 GGCGCC GGC^GCC
SspBI 5362 TGTACA T^GTACA
SspI 5302 AATATT AAT^ATT
Tth111I 2716 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4401 TCTAGA T^CTAGA
XhoI 4019 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AhdI 931 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACCTACTGTC
Asp700I 993 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1128 GAAGCTGTTC
AspEI 931 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACCTACTGTC
BcgI 3840 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
195 GCAAAGGTGTCG
BsaWI 227 TCCGGT [AT]^CCGG[AT]
3718 ACCGGT
BspLU11I 786 ACATGT A^CATGT
2929 ACATGT
BssSI 3536 CTCGTG CACGAG(-5/-1)
5378 CTCGTG
DsaI 1355 CCACGG C^C[GA][CT]GG
2987 CCATGG
Eam1105I 931 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACCTACTGTC
HgaI 410 GCGTC GACGC(5/10)
4592 GCGTC
HindIII 995 AAGCTT A^AGCTT
2606 AAGCTT
SmaI 270 CCCGGG CCC^GGG
3944 CCCGGG
SmlI 2813 CTTGAG C^T[CT][GA]AG
4019 CTCGAG
XmaCI 270 CCCGGG C^CCGGG
3944 CCCGGG
XmaI 270 CCCGGG C^CCGGG
3944 CCCGGG
XmnI 993 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1128 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AccII AciI AfaI AflIII
AluI AlwI AlwNI ApaI Asp718I
AspHI AvaI AvaII AviII BalI
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
FspI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII