################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccI AccIII AclI AflII AocI AscI BbrPI BciVI BfrI BsaBI BseAI BspDI BspEI BspHI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII DrdI Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HpaI KspI MamI MluI MroI NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcsI 515 AAATTC [GA]^AATT[CT] AcyI 343 GGCGCC G[GA]^CG[CT]C AgeI 3718 ACCGGT A^CCGGT Alw44I 2753 GTGCAC G^TGCAC Aor51HI 5021 AGCGCT AGC^GCT ApaLI 2753 GTGCAC G^TGCAC ApoI 515 AAATTC [GA]^AATT[CT] AseI 5285 ATTAAT AT^TAAT AsnI 5285 ATTAAT AT^TAAT AspI 2716 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1721 CCTAGG C^CTAGG BamHI 2976 GGATCC G^GATCC BbeI 343 GGCGCC GGCGC^C BglII 3215 AGATCT A^GATCT BlnI 1721 CCTAGG C^CTAGG BsaAI 933 CACGTA [CT]AC^GT[GA] BsaHI 343 GGCGCC G[GA]^CG[CT]C BsiEI 380 CGGCCG CG[GA][CT]^CG BsiWI 3121 CGTACG C^GTACG BsmBI 2721 CGTCTC CGTCTC(1/5) Bsp1407I 5362 TGTACA T^GTACA BsrGI 5362 TGTACA T^GTACA BssHII 406 GCGCGC G^CGCGC BstAPI 5239 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 5614 TTTAAA TTT^AAA EagI 380 CGGCCG C^GGCCG EclXI 380 CGGCCG C^GGCCG Eco47III 5021 AGCGCT AGC^GCT Eco52I 380 CGGCCG C^GGCCG Hin1I 343 GGCGCC G[GA]^CG[CT]C KasI 343 GGCGCC G^GCGCC MfeI 953 CAATTG C^AATTG MunI 953 CAATTG C^AATTG NaeI 181 GCCGGC GCC^GGC NarI 343 GGCGCC GG^CGCC NcoI 2987 CCATGG C^CATGG NdeI 4866 CATATG CA^TATG NgoMIV 181 GCCGGC G^CCGGC PaeR7I 4019 CTCGAG C^TCGAG PflFI 2716 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3718 ACCGGT A^CCGGT PshAI 3169 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5285 ATTAAT AT^TAAT PstI 2833 CTGCAG CTGCA^G SapI 308 GAAGAGC GCTCTTC(1/4) ScaI 1275 AGTACT AGT^ACT SfoI 343 GGCGCC GGC^GCC SspBI 5362 TGTACA T^GTACA SspI 5302 AATATT AAT^ATT Tth111I 2716 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4401 TCTAGA T^CTAGA XhoI 4019 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AhdI 931 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2128 GACCTACTGTC Asp700I 993 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1128 GAAGCTGTTC AspEI 931 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2128 GACCTACTGTC BcgI 3840 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 195 GCAAAGGTGTCG BsaWI 227 TCCGGT [AT]^CCGG[AT] 3718 ACCGGT BspLU11I 786 ACATGT A^CATGT 2929 ACATGT BssSI 3536 CTCGTG CACGAG(-5/-1) 5378 CTCGTG DsaI 1355 CCACGG C^C[GA][CT]GG 2987 CCATGG Eam1105I 931 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2128 GACCTACTGTC HgaI 410 GCGTC GACGC(5/10) 4592 GCGTC HindIII 995 AAGCTT A^AGCTT 2606 AAGCTT SmaI 270 CCCGGG CCC^GGG 3944 CCCGGG SmlI 2813 CTTGAG C^T[CT][GA]AG 4019 CTCGAG XmaCI 270 CCCGGG C^CCGGG 3944 CCCGGG XmaI 270 CCCGGG C^CCGGG 3944 CCCGGG XmnI 993 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1128 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### Acc65I AccII AciI AfaI AflIII AluI AlwI AlwNI ApaI Asp718I AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII