################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## Acc65I AclI AflII AscI AseI AsnI Asp718I BfrI BsiWI Bsp1407I BsrGI BssHII Bst1107I BstZ17I CpoI EagI EclXI Eco52I EcoRI EcoT22I FseI HpaI KpnI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI PacI PmeI PshBI Psp1406I PvuI RsrII SalI SgrAI SpeI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 2341 TCCGGA T^CCGGA AflIII 4113 ACATGT A^C[GA][CT]GT AgeI 1427 ACCGGT A^CCGGT Aor51HI 3100 AGCGCT AGC^GCT Asp700I 3410 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 29 TGCGCA TGC^GCA AvrII 3959 CCTAGG C^CTAGG BbrPI 3093 CACGTG CAC^GTG BciVI 4542 GGATAC GTATCC(6/5) BclI 5704 TGATCA T^GATCA BlnI 3959 CCTAGG C^CTAGG BsaBI 669 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2341 TCCGGA T^CCGGA BsiEI 1907 CGACCG CG[GA][CT]^CG BsmBI 5097 GAGACG CGTCTC(1/5) BsmI 5675 GAATGC GAATGC(1/-1) BspDI 4971 ATCGAT AT^CGAT BspEI 2341 TCCGGA T^CCGGA BspLU11I 4113 ACATGT A^CATGT BstBI 3282 TTCGAA TT^CGAA ClaI 4971 ATCGAT AT^CGAT DraI 4652 TTTAAA TTT^AAA DrdI 2313 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3100 AGCGCT AGC^GCT FbaI 5704 TGATCA T^GATCA FspI 29 TGCGCA TGC^GCA HindIII 178 AAGCTT A^AGCTT KspI 2565 CCGCGG CCGC^GG MamI 669 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2341 TCCGGA T^CCGGA MseI 4653 TTAA T^TAA NdeI 2974 CATATG CA^TATG NspV 3282 TTCGAA TT^CGAA PinAI 1427 ACCGGT A^CCGGT PmaCI 3093 CACGTG CAC^GTG PmlI 3093 CACGTG CAC^GTG PshAI 1242 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 2565 CCGCGG CCGC^GG ScaI 1837 AGTACT AGT^ACT SfuI 3282 TTCGAA TT^CGAA SnaBI 4545 TACGTA TAC^GTA SphI 965 GCATGC GCATG^C Tru9I 4653 TTAA T^TAA XmnI 3410 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 2115 GACGTC GACGT^C 5099 GACGTC AcsI 541 AAATTT [GA]^AATT[CT] 2085 AAATTC Alw44I 3053 GTGCAC G^TGCAC 3620 GTGCAC AocI 117 CCTCAGG CC^T[ACGT]AGG 5710 CCTCAGG ApaLI 3053 GTGCAC G^TGCAC 3620 GTGCAC ApoI 541 AAATTT [GA]^AATT[CT] 2085 AAATTC BamHI 1792 GGATCC G^GATCC 3551 GGATCC BbeI 137 GGCGCC GGCGC^C 1543 GGCGCC BcgI 2651 CGAGGTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3117 CGAAAGCACTGC BsaAI 3093 CACGTG [CT]AC^GT[GA] 4545 TACGTA BsaWI 1427 ACCGGT [AT]^CCGG[AT] 2341 TCCGGA BspHI 2464 TCATGA T^CATGA 3514 TCATGA Bsu36I 117 CCTCAGG CC^T[ACGT]AGG 5710 CCTCAGG DraIII 431 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG 3198 CACCTGGTG Eco81I 117 CCTCAGG CC^T[ACGT]AGG 5710 CCTCAGG EcoRV 4231 GATATC GAT^ATC 4989 GATATC HincII 2130 GTCAAC GT[CT]^[GA]AC 2910 GTCAAC HindII 2130 GTCAAC GT[CT]^[GA]AC 2910 GTCAAC KasI 137 GGCGCC G^GCGCC 1543 GGCGCC NarI 137 GGCGCC GG^CGCC 1543 GGCGCC PaeR7I 142 CTCGAG C^TCGAG 5233 CTCGAG PflMI 1494 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1647 CCAGCAGGTGG RcaI 2464 TCATGA T^CATGA 3514 TCATGA SfoI 137 GGCGCC GGC^GCC 1543 GGCGCC SmaI 83 CCCGGG CCC^GGG 1109 CCCGGG Van91I 1494 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1647 CCAGCAGGTGG XhoI 142 CTCGAG C^TCGAG 5233 CTCGAG XmaCI 83 CCCGGG C^CCGGG 1109 CCCGGG XmaI 83 CCCGGG C^CCGGG 1109 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI ApaI AspEI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SfiI SmlI Sse8387I StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII