##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AclI AflII AscI AseI
AsnI Asp718I BfrI BsiWI Bsp1407I
BsrGI BssHII Bst1107I BstZ17I CpoI
EagI EclXI Eco52I EcoRI EcoT22I
FseI HpaI KpnI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PshBI
Psp1406I PvuI RsrII SalI SgrAI
SpeI SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 2341 TCCGGA T^CCGGA
AflIII 4113 ACATGT A^C[GA][CT]GT
AgeI 1427 ACCGGT A^CCGGT
Aor51HI 3100 AGCGCT AGC^GCT
Asp700I 3410 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 29 TGCGCA TGC^GCA
AvrII 3959 CCTAGG C^CTAGG
BbrPI 3093 CACGTG CAC^GTG
BciVI 4542 GGATAC GTATCC(6/5)
BclI 5704 TGATCA T^GATCA
BlnI 3959 CCTAGG C^CTAGG
BsaBI 669 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2341 TCCGGA T^CCGGA
BsiEI 1907 CGACCG CG[GA][CT]^CG
BsmBI 5097 GAGACG CGTCTC(1/5)
BsmI 5675 GAATGC GAATGC(1/-1)
BspDI 4971 ATCGAT AT^CGAT
BspEI 2341 TCCGGA T^CCGGA
BspLU11I 4113 ACATGT A^CATGT
BstBI 3282 TTCGAA TT^CGAA
ClaI 4971 ATCGAT AT^CGAT
DraI 4652 TTTAAA TTT^AAA
DrdI 2313 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3100 AGCGCT AGC^GCT
FbaI 5704 TGATCA T^GATCA
FspI 29 TGCGCA TGC^GCA
HindIII 178 AAGCTT A^AGCTT
KspI 2565 CCGCGG CCGC^GG
MamI 669 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2341 TCCGGA T^CCGGA
MseI 4653 TTAA T^TAA
NdeI 2974 CATATG CA^TATG
NspV 3282 TTCGAA TT^CGAA
PinAI 1427 ACCGGT A^CCGGT
PmaCI 3093 CACGTG CAC^GTG
PmlI 3093 CACGTG CAC^GTG
PshAI 1242 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2565 CCGCGG CCGC^GG
ScaI 1837 AGTACT AGT^ACT
SfuI 3282 TTCGAA TT^CGAA
SnaBI 4545 TACGTA TAC^GTA
SphI 965 GCATGC GCATG^C
Tru9I 4653 TTAA T^TAA
XmnI 3410 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 2115 GACGTC GACGT^C
5099 GACGTC
AcsI 541 AAATTT [GA]^AATT[CT]
2085 AAATTC
Alw44I 3053 GTGCAC G^TGCAC
3620 GTGCAC
AocI 117 CCTCAGG CC^T[ACGT]AGG
5710 CCTCAGG
ApaLI 3053 GTGCAC G^TGCAC
3620 GTGCAC
ApoI 541 AAATTT [GA]^AATT[CT]
2085 AAATTC
BamHI 1792 GGATCC G^GATCC
3551 GGATCC
BbeI 137 GGCGCC GGCGC^C
1543 GGCGCC
BcgI 2651 CGAGGTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3117 CGAAAGCACTGC
BsaAI 3093 CACGTG [CT]AC^GT[GA]
4545 TACGTA
BsaWI 1427 ACCGGT [AT]^CCGG[AT]
2341 TCCGGA
BspHI 2464 TCATGA T^CATGA
3514 TCATGA
Bsu36I 117 CCTCAGG CC^T[ACGT]AGG
5710 CCTCAGG
DraIII 431 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3198 CACCTGGTG
Eco81I 117 CCTCAGG CC^T[ACGT]AGG
5710 CCTCAGG
EcoRV 4231 GATATC GAT^ATC
4989 GATATC
HincII 2130 GTCAAC GT[CT]^[GA]AC
2910 GTCAAC
HindII 2130 GTCAAC GT[CT]^[GA]AC
2910 GTCAAC
KasI 137 GGCGCC G^GCGCC
1543 GGCGCC
NarI 137 GGCGCC GG^CGCC
1543 GGCGCC
PaeR7I 142 CTCGAG C^TCGAG
5233 CTCGAG
PflMI 1494 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1647 CCAGCAGGTGG
RcaI 2464 TCATGA T^CATGA
3514 TCATGA
SfoI 137 GGCGCC GGC^GCC
1543 GGCGCC
SmaI 83 CCCGGG CCC^GGG
1109 CCCGGG
Van91I 1494 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1647 CCAGCAGGTGG
XhoI 142 CTCGAG C^TCGAG
5233 CTCGAG
XmaCI 83 CCCGGG C^CCGGG
1109 CCCGGG
XmaI 83 CCCGGG C^CCGGG
1109 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AhdI AluI AlwI AlwNI ApaI
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfiI SmlI Sse8387I StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII