################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AclI AflII AhdI Aor51HI AscI AseI AsnI Asp718I AspEI BamHI BciVI BfrI BglII BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI HpaI KpnI MamI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SexAI SnaBI SpeI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccI 4971 GTCTAC GT^[AC][GT]AC AgeI 1433 ACCGGT A^CCGGT Alw44I 3699 GTGCAC G^TGCAC ApaLI 3699 GTGCAC G^TGCAC AviII 253 TGCGCA TGC^GCA BbeI 65 GGCGCC GGCGC^C BclI 343 TGATCA T^GATCA BsmBI 2579 CGTCTC CGTCTC(1/5) BstBI 780 TTCGAA TT^CGAA EcoRV 264 GATATC GAT^ATC FbaI 343 TGATCA T^GATCA FspI 253 TGCGCA TGC^GCA KasI 65 GGCGCC G^GCGCC KspI 353 CCGCGG CCGC^GG MfeI 1123 CAATTG C^AATTG MunI 1123 CAATTG C^AATTG NarI 65 GGCGCC GG^CGCC NdeI 2272 CATATG CA^TATG NspV 780 TTCGAA TT^CGAA PaeR7I 528 CTCGAG C^TCGAG PinAI 1433 ACCGGT A^CCGGT SacII 353 CCGCGG CCGC^GG ScaI 3643 AGTACT AGT^ACT SfoI 65 GGCGCC GGC^GCC SfuI 780 TTCGAA TT^CGAA SmaI 1634 CCCGGG CCC^GGG XhoI 528 CTCGAG C^TCGAG XmaCI 1634 CCCGGG C^CCGGG XmaI 1634 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccII 16 CGCG CG^CG 354 CGCG AccIII 2641 TCCGGA T^CCGGA 3804 TCCGGA AcyI 65 GGCGCC G[GA]^CG[CT]C 285 GGCGTC ApaI 3071 GGGCCC GGGCC^C 3588 GGGCCC Asp700I 962 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1863 GAAAACCTTC AspI 1364 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1424 GACATTGTC AvrII 1679 CCTAGG C^CTAGG 5427 CCTAGG BbrPI 1914 CACGTG CAC^GTG 3153 CACGTG BcgI 398 GCAGCAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3007 GCAAGCTCCTCG BglI 3064 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5043 GCCGCTGTGGC BlnI 1679 CCTAGG C^CTAGG 5427 CCTAGG BsaHI 65 GGCGCC G[GA]^CG[CT]C 285 GGCGTC BseAI 2641 TCCGGA T^CCGGA 3804 TCCGGA BspEI 2641 TCCGGA T^CCGGA 3804 TCCGGA BspLU11I 3631 ACATGT A^CATGT 5248 ACATGT BsrBI 495 GAGCGG CCGCTC(-3/-3) 4988 GAGCGG BsrDI 1568 CATTGC GCAATG(2/0) 5316 CATTGC BssSI 375 CACGAG CACGAG(-5/-1) 2073 CACGAG BstEII 1739 GGTGACC G^GT[ACGT]ACC 4502 GGTGACC BstPI 1739 GGTGACC G^GT[ACGT]ACC 4502 GGTGACC BstUI 16 CGCG CG^CG 354 CGCG EcoNI 1679 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4250 CCTGCACAAGG EcoO65I 1739 GGTGACC G^GT[ACGT]ACC 4502 GGTGACC HaeII 65 GGCGCC [GA]GCGC^[CT] 370 AGCGCC Hin1I 65 GGCGCC G[GA]^CG[CT]C 285 GGCGTC HincII 874 GTCAAC GT[CT]^[GA]AC 3104 GTTGAC HindII 874 GTCAAC GT[CT]^[GA]AC 3104 GTTGAC MroI 2641 TCCGGA T^CCGGA 3804 TCCGGA MvnI 16 CGCG CG^CG 354 CGCG PflFI 1364 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1424 GACATTGTC PmaCI 1914 CACGTG CAC^GTG 3153 CACGTG PmlI 1914 CACGTG CAC^GTG 3153 CACGTG SapI 3123 GAAGAGC GCTCTTC(1/4) 5338 GCTCTTC SfiI 3063 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5042 GGCCGCTGTGGCC SgrAI 281 CACCGGCG C[GA]^CCGG[CT]G 1432 CACCGGTG SphI 3529 GCATGC GCATG^C 3580 GCATGC Tth111I 1364 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1424 GACATTGTC XmnI 962 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1863 GAAAACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII