##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI BamHI BciVI BfrI BglII
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FseI HpaI KpnI MamI MluI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SexAI SnaBI SpeI SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 4971 GTCTAC GT^[AC][GT]AC
AgeI 1433 ACCGGT A^CCGGT
Alw44I 3699 GTGCAC G^TGCAC
ApaLI 3699 GTGCAC G^TGCAC
AviII 253 TGCGCA TGC^GCA
BbeI 65 GGCGCC GGCGC^C
BclI 343 TGATCA T^GATCA
BsmBI 2579 CGTCTC CGTCTC(1/5)
BstBI 780 TTCGAA TT^CGAA
EcoRV 264 GATATC GAT^ATC
FbaI 343 TGATCA T^GATCA
FspI 253 TGCGCA TGC^GCA
KasI 65 GGCGCC G^GCGCC
KspI 353 CCGCGG CCGC^GG
MfeI 1123 CAATTG C^AATTG
MunI 1123 CAATTG C^AATTG
NarI 65 GGCGCC GG^CGCC
NdeI 2272 CATATG CA^TATG
NspV 780 TTCGAA TT^CGAA
PaeR7I 528 CTCGAG C^TCGAG
PinAI 1433 ACCGGT A^CCGGT
SacII 353 CCGCGG CCGC^GG
ScaI 3643 AGTACT AGT^ACT
SfoI 65 GGCGCC GGC^GCC
SfuI 780 TTCGAA TT^CGAA
SmaI 1634 CCCGGG CCC^GGG
XhoI 528 CTCGAG C^TCGAG
XmaCI 1634 CCCGGG C^CCGGG
XmaI 1634 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 16 CGCG CG^CG
354 CGCG
AccIII 2641 TCCGGA T^CCGGA
3804 TCCGGA
AcyI 65 GGCGCC G[GA]^CG[CT]C
285 GGCGTC
ApaI 3071 GGGCCC GGGCC^C
3588 GGGCCC
Asp700I 962 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1863 GAAAACCTTC
AspI 1364 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1424 GACATTGTC
AvrII 1679 CCTAGG C^CTAGG
5427 CCTAGG
BbrPI 1914 CACGTG CAC^GTG
3153 CACGTG
BcgI 398 GCAGCAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3007 GCAAGCTCCTCG
BglI 3064 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5043 GCCGCTGTGGC
BlnI 1679 CCTAGG C^CTAGG
5427 CCTAGG
BsaHI 65 GGCGCC G[GA]^CG[CT]C
285 GGCGTC
BseAI 2641 TCCGGA T^CCGGA
3804 TCCGGA
BspEI 2641 TCCGGA T^CCGGA
3804 TCCGGA
BspLU11I 3631 ACATGT A^CATGT
5248 ACATGT
BsrBI 495 GAGCGG CCGCTC(-3/-3)
4988 GAGCGG
BsrDI 1568 CATTGC GCAATG(2/0)
5316 CATTGC
BssSI 375 CACGAG CACGAG(-5/-1)
2073 CACGAG
BstEII 1739 GGTGACC G^GT[ACGT]ACC
4502 GGTGACC
BstPI 1739 GGTGACC G^GT[ACGT]ACC
4502 GGTGACC
BstUI 16 CGCG CG^CG
354 CGCG
EcoNI 1679 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4250 CCTGCACAAGG
EcoO65I 1739 GGTGACC G^GT[ACGT]ACC
4502 GGTGACC
HaeII 65 GGCGCC [GA]GCGC^[CT]
370 AGCGCC
Hin1I 65 GGCGCC G[GA]^CG[CT]C
285 GGCGTC
HincII 874 GTCAAC GT[CT]^[GA]AC
3104 GTTGAC
HindII 874 GTCAAC GT[CT]^[GA]AC
3104 GTTGAC
MroI 2641 TCCGGA T^CCGGA
3804 TCCGGA
MvnI 16 CGCG CG^CG
354 CGCG
PflFI 1364 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1424 GACATTGTC
PmaCI 1914 CACGTG CAC^GTG
3153 CACGTG
PmlI 1914 CACGTG CAC^GTG
3153 CACGTG
SapI 3123 GAAGAGC GCTCTTC(1/4)
5338 GCTCTTC
SfiI 3063 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5042 GGCCGCTGTGGCC
SgrAI 281 CACCGGCG C[GA]^CCGG[CT]G
1432 CACCGGTG
SphI 3529 GCATGC GCATG^C
3580 GCATGC
Tth111I 1364 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1424 GACATTGTC
XmnI 962 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1863 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII