##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AcsI AflII AflIII ApoI
AscI AseI AsnI AviII AvrII
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI BsmI BspDI BspLU11I
BssHII BstBI ClaI CpoI DraI
DrdI EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MluI MseI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I RsrII SalI
SapI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1671 GACGTC GACGT^C
Acc65I 298 GGTACC G^GTACC
AccI 783 GTATAC GT^[AC][GT]AC
AgeI 376 ACCGGT A^CCGGT
AhdI 2616 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 825 GTGCAC G^TGCAC
AocI 282 CCTGAGG CC^T[ACGT]AGG
ApaLI 825 GTGCAC G^TGCAC
Asp700I 2738 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 298 GGTACC G^GTACC
AspEI 2616 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1266 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1244 GGATCC G^GATCC
BbeI 3067 GGCGCC GGCGC^C
BbrPI 687 CACGTG CAC^GTG
BbsI 425 GAAGAC GAAGAC(2/6)
BcgI 2570 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 26 CTAG C^TAG
BpuAI 425 GAAGAC GAAGAC(2/6)
BsaI 2878 GAGACC GGTCTC(1/5)
Bsp1407I 1744 TGTACA T^GTACA
BspHI 178 TCATGA T^CATGA
BsrGI 1744 TGTACA T^GTACA
Bst1107I 783 GTATAC GTA^TAC
BstEII 1808 GGTGACC G^GT[ACGT]ACC
BstPI 1808 GGTGACC G^GT[ACGT]ACC
BstXI 1456 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 783 GTATAC GTA^TAC
Bsu36I 282 CCTGAGG CC^T[ACGT]AGG
DraIII 2340 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2943 CGGCCG C^GGCCG
Eam1105I 2616 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2943 CGGCCG C^GGCCG
Eco52I 2943 CGGCCG C^GGCCG
Eco81I 282 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1808 GGTGACC G^GT[ACGT]ACC
HgaI 3028 GCGTC GACGC(5/10)
HincII 1314 GTCAAC GT[CT]^[GA]AC
HindII 1314 GTCAAC GT[CT]^[GA]AC
KasI 3067 GGCGCC G^GCGCC
KpnI 298 GGTACC GGTAC^C
KspI 2903 CCGCGG CCGC^GG
MaeI 26 CTAG C^TAG
NaeI 96 GCCGGC GCC^GGC
NarI 3067 GGCGCC GG^CGCC
NgoMIV 96 GCCGGC G^CCGGC
NspI 2527 ACATGC [GA]CATG^[CT]
PflFI 1266 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 376 ACCGGT A^CCGGT
PmaCI 687 CACGTG CAC^GTG
PmlI 687 CACGTG CAC^GTG
PshAI 2195 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 631 CGATCG CGAT^CG
RcaI 178 TCATGA T^CATGA
SacI 312 GAGCTC GAGCT^C
SacII 2903 CCGCGG CCGC^GG
SexAI 1963 ACCAGGT A^CC[AT]GGT
SfiI 2543 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3067 GGCGCC GGC^GCC
SspBI 1744 TGTACA T^GTACA
Tth111I 1266 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2738 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1671 GACGTC G[GA]^CG[CT]C
3067 GGCGCC
Aor51HI 2118 AGCGCT AGC^GCT
3209 AGCGCT
BalI 346 TGGCCA TGG^CCA
2162 TGGCCA
BsaAI 687 CACGTG [CT]AC^GT[GA]
1676 CACGTA
BsaHI 1671 GACGTC G[GA]^CG[CT]C
3067 GGCGCC
BsiEI 631 CGATCG CG[GA][CT]^CG
2943 CGGCCG
BsmBI 3029 CGTCTC CGTCTC(1/5)
969 GAGACG
BsrDI 366 GCAATG GCAATG(2/0)
550 GCAATG
BssSI 1065 CTCGTG CACGAG(-5/-1)
2919 CTCGTG
Eco47III 2118 AGCGCT AGC^GCT
3209 AGCGCT
Hin1I 1671 GACGTC G[GA]^CG[CT]C
3067 GGCGCC
MfeI 720 CAATTG C^AATTG
3192 CAATTG
MluNI 346 TGGCCA TGG^CCA
2162 TGGCCA
MscI 346 TGGCCA TGG^CCA
2162 TGGCCA
MunI 720 CAATTG C^AATTG
3192 CAATTG
PflMI 2087 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2882 CCAGCTGGTGG
StuI 541 AGGCCT AGG^CCT
1513 AGGCCT
Van91I 2087 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2882 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MroI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI