##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I AocI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BclI BlnI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrFI BssSI BstBI Bsu36I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I Eco81I EcoRV FbaI FseI
FspI HincII HindII HpaI KasI
KpnI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1483 GACGTC GACGT^C
AflII 4222 CTTAAG C^TTAAG
Aor51HI 18 AGCGCT AGC^GCT
AvaI 1922 CCCGGG C^[CT]CG[GA]G
BamHI 740 GGATCC G^GATCC
BbrPI 329 CACGTG CAC^GTG
BbvCI 14 CCTCAGC CCTCAGC(-5/-2)
BfrI 4222 CTTAAG C^TTAAG
BglII 4751 AGATCT A^GATCT
BsaBI 597 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1738 TCCGGT [AT]^CCGG[AT]
BsmI 4890 GCATTC GAATGC(1/-1)
BsoBI 1922 CCCGGG C^[CT]CG[GA]G
BssHII 2070 GCGCGC G^CGCGC
BstEII 3364 GGTTACC G^GT[ACGT]ACC
BstPI 3364 GGTTACC G^GT[ACGT]ACC
DraIII 2311 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 18 AGCGCT AGC^GCT
EcoO65I 3364 GGTTACC G^GT[ACGT]ACC
EcoT22I 4065 ATGCAT ATGCA^T
MamI 597 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4852 CCATGG C^CATGG
NdeI 3222 CATATG CA^TATG
NsiI 4065 ATGCAT ATGCA^T
PmaCI 329 CACGTG CAC^GTG
PmlI 329 CACGTG CAC^GTG
SapI 1593 GAAGAGC GCTCTTC(1/4)
SbfI 2001 CCTGCAGG CCTGCA^GG
SexAI 458 ACCAGGT A^CC[AT]GGT
SmaI 1922 CCCGGG CCC^GGG
SpeI 1969 ACTAGT A^CTAGT
SphI 4496 GCATGC GCATG^C
Sse8387I 2001 CCTGCAGG CCTGCA^GG
SwaI 5171 ATTTAAAT ATTT^AAAT
XmaCI 1922 CCCGGG C^CCGGG
XmaI 1922 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1483 GACGTC G[GA]^CG[CT]C
2213 GGCGTC
AseI 570 ATTAAT AT^TAAT
2758 ATTAAT
AsnI 570 ATTAAT AT^TAAT
2758 ATTAAT
BcgI 697 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1086 GCAGACCGTTCG
BglI 695 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4084 GCCTGGAAGGC
BsaAI 329 CACGTG [CT]AC^GT[GA]
2474 TACGTG
BsaHI 1483 GACGTC G[GA]^CG[CT]C
2213 GGCGTC
Bsp1407I 995 TGTACA T^GTACA
3608 TGTACA
BspHI 2655 TCATGA T^CATGA
4162 TCATGA
BsrBI 271 CCGCTC CCGCTC(-3/-3)
7 GAGCGG
BsrGI 995 TGTACA T^GTACA
3608 TGTACA
BstXI 175 CCAGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3986 CCAACTACATGG
DrdI 1939 GACCCGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2979 GACCCTAGAGTC
EcoRI 2013 GAATTC G^AATTC
3717 GAATTC
HaeII 18 AGCGCT [GA]GCGC^[CT]
1566 GGCGCT
HgaI 115 GACGC GACGC(5/10)
2214 GCGTC
Hin1I 1483 GACGTC G[GA]^CG[CT]C
2213 GGCGTC
KspI 57 CCGCGG CCGC^GG
1942 CCGCGG
MfeI 3525 CAATTG C^AATTG
4677 CAATTG
MunI 3525 CAATTG C^AATTG
4677 CAATTG
NspI 965 ACATGC [GA]CATG^[CT]
4496 GCATGC
PshBI 570 ATTAAT AT^TAAT
2758 ATTAAT
RcaI 2655 TCATGA T^CATGA
4162 TCATGA
SacI 828 GAGCTC GAGCT^C
1276 GAGCTC
SacII 57 CCGCGG CCGC^GG
1942 CCGCGG
SspBI 995 TGTACA T^GTACA
3608 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstUI
BstYI BstZ17I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI