##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccI AclI AflII AscI
AseI AsnI Asp718I BclI BfrI
BglII BsiWI BsmI BspDI BsrDI
Bst1107I BstZ17I ClaI CpoI DraI
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI KspI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NsiI PacI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI SnaBI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3318 GACGTC GACGT^C
AccIII 65 TCCGGA T^CCGGA
AgeI 2943 ACCGGT A^CCGGT
AhdI 3363 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3363 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1347 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2843 CACGTG CAC^GTG
BsaAI 2843 CACGTG [CT]AC^GT[GA]
BsaBI 3858 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 65 TCCGGA T^CCGGA
Bsp1407I 953 TGTACA T^GTACA
BspEI 65 TCCGGA T^CCGGA
BsrGI 953 TGTACA T^GTACA
BssHII 319 GCGCGC G^CGCGC
BstBI 416 TTCGAA TT^CGAA
Eam1105I 3363 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MamI 3858 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 65 TCCGGA T^CCGGA
MseI 967 TTAA T^TAA
NdeI 98 CATATG CA^TATG
NruI 3372 TCGCGA TCG^CGA
NspV 416 TTCGAA TT^CGAA
PflFI 1347 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2943 ACCGGT A^CCGGT
PmaCI 2843 CACGTG CAC^GTG
PmlI 2843 CACGTG CAC^GTG
SapI 3994 GAAGAGC GCTCTTC(1/4)
ScaI 161 AGTACT AGT^ACT
SfuI 416 TTCGAA TT^CGAA
SmaI 1066 CCCGGG CCC^GGG
SpeI 555 ACTAGT A^CTAGT
SphI 3575 GCATGC GCATG^C
SspBI 953 TGTACA T^GTACA
Tru9I 967 TTAA T^TAA
Tth111I 1347 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 695 TCTAGA T^CTAGA
XmaCI 1066 CCCGGG C^CCGGG
XmaI 1066 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 229 GTGCAC G^TGCAC
2651 GTGCAC
ApaLI 229 GTGCAC G^TGCAC
2651 GTGCAC
BbeI 474 GGCGCC GGCGC^C
2933 GGCGCC
BbvCI 4520 CCTCAGC CCTCAGC(-5/-2)
304 GCTGAGG
BciVI 448 GTATCC GTATCC(6/5)
1567 GTATCC
BlpI 273 GCTCAGC GC^T[ACGT]AGC
2891 GCTGAGC
Bpu1102I 273 GCTCAGC GC^T[ACGT]AGC
2891 GCTGAGC
BspHI 2499 TCATGA T^CATGA
4993 TCATGA
BspLU11I 335 ACATGT A^CATGT
5688 ACATGT
BstEII 4309 GGTCACC G^GT[ACGT]ACC
4708 GGTCACC
BstPI 4309 GGTCACC G^GT[ACGT]ACC
4708 GGTCACC
CelII 273 GCTCAGC GC^T[ACGT]AGC
2891 GCTGAGC
DraIII 1574 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2091 CACCCAGTG
DrdI 123 GACCAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2990 GACGCCCACGTC
EagI 1105 CGGCCG C^GGCCG
5501 CGGCCG
EclXI 1105 CGGCCG C^GGCCG
5501 CGGCCG
Eco52I 1105 CGGCCG C^GGCCG
5501 CGGCCG
EcoO65I 4309 GGTCACC G^GT[ACGT]ACC
4708 GGTCACC
HindIII 587 AAGCTT A^AGCTT
3433 AAGCTT
KasI 474 GGCGCC G^GCGCC
2933 GGCGCC
NarI 474 GGCGCC GG^CGCC
2933 GGCGCC
PaeR7I 3021 CTCGAG C^TCGAG
3295 CTCGAG
RcaI 2499 TCATGA T^CATGA
4993 TCATGA
SacI 3673 GAGCTC GAGCT^C
4465 GAGCTC
SfoI 474 GGCGCC GGC^GCC
2933 GGCGCC
XhoI 3021 CTCGAG C^TCGAG
3295 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI Asp700I AspHI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI