##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AflII AscI AseI AsnI
Asp718I BamHI BfrI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspLU11I BsrGI
BssHII BstAPI BstBI CelII EarI
FseI HpaI KpnI Ksp632I MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PmeI PshAI
PshBI PvuI SalI SapI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 3950 GACGTC GACGT^C
AccIII 1317 TCCGGA T^CCGGA
AclI 4157 AACGTT AA^CGTT
AflIII 3323 ACGTGT A^C[GA][CT]GT
AhdI 236 GACGATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3452 AGCGCT AGC^GCT
AspEI 236 GACGATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4700 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1326 TGCGCA TGC^GCA
AvrII 4484 CCTAGG C^CTAGG
BbeI 87 GGCGCC GGCGC^C
BbrPI 3322 CACGTG CAC^GTG
BciVI 2525 GGATAC GTATCC(6/5)
BglII 1447 AGATCT A^GATCT
BlnI 4484 CCTAGG C^CTAGG
BsaAI 3322 CACGTG [CT]AC^GT[GA]
BseAI 1317 TCCGGA T^CCGGA
BsiEI 2830 CGGCCG CG[GA][CT]^CG
BsmI 5744 GCATTC GAATGC(1/-1)
BspDI 2696 ATCGAT AT^CGAT
BspEI 1317 TCCGGA T^CCGGA
BspHI 402 TCATGA T^CATGA
BspMI 750 ACCTGC ACCTGC(4/8)
BssSI 2442 CTCGTG CACGAG(-5/-1)
Bst1107I 3923 GTATAC GTA^TAC
BstZ17I 3923 GTATAC GTA^TAC
ClaI 2696 ATCGAT AT^CGAT
CpoI 4698 CGGACCG CG^G[AT]CCG
DraIII 764 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2830 CGGCCG C^GGCCG
Eam1105I 236 GACGATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2830 CGGCCG C^GGCCG
Eco47III 3452 AGCGCT AGC^GCT
Eco52I 2830 CGGCCG C^GGCCG
EcoRV 4067 GATATC GAT^ATC
FspI 1326 TGCGCA TGC^GCA
KasI 87 GGCGCC G^GCGCC
KspI 1702 CCGCGG CCGC^GG
MroI 1317 TCCGGA T^CCGGA
NaeI 4309 GCCGGC GCC^GGC
NarI 87 GGCGCC GG^CGCC
NgoMIV 4309 GCCGGC G^CCGGC
PacI 5701 TTAATTAA TTAAT^TAA
PflFI 4700 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3322 CACGTG CAC^GTG
PmlI 3322 CACGTG CAC^GTG
Psp1406I 4157 AACGTT AA^CGTT
RcaI 402 TCATGA T^CATGA
RsrII 4698 CGGACCG CG^G[AT]CCG
SacI 3983 GAGCTC GAGCT^C
SacII 1702 CCGCGG CCGC^GG
SfoI 87 GGCGCC GGC^GCC
SspI 5734 AATATT AAT^ATT
Tth111I 4700 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AgeI 2093 ACCGGT A^CCGGT
2372 ACCGGT
Alw44I 2259 GTGCAC G^TGCAC
5679 GTGCAC
ApaLI 2259 GTGCAC G^TGCAC
5679 GTGCAC
BbvCI 3679 CCTCAGC CCTCAGC(-5/-2)
4650 CCTCAGC
BclI 822 TGATCA T^GATCA
4660 TGATCA
BsmBI 2450 GAGACG CGTCTC(1/5)
2469 GAGACG
BstXI 600 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4335 CCAAGCCTGTGG
DraI 5713 TTTAAA TTT^AAA
5808 TTTAAA
EcoT22I 1034 ATGCAT ATGCA^T
1550 ATGCAT
FbaI 822 TGATCA T^GATCA
4660 TGATCA
HincII 1532 GTCAAC GT[CT]^[GA]AC
2813 GTCAAC
HindII 1532 GTCAAC GT[CT]^[GA]AC
2813 GTCAAC
HindIII 1074 AAGCTT A^AGCTT
1656 AAGCTT
NcoI 2109 CCATGG C^CATGG
4536 CCATGG
NsiI 1034 ATGCAT ATGCA^T
1550 ATGCAT
PaeR7I 1307 CTCGAG C^TCGAG
1998 CTCGAG
PflMI 199 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3359 CCATCCTGTGG
PinAI 2093 ACCGGT A^CCGGT
2372 ACCGGT
SbfI 3281 CCTGCAGG CCTGCA^GG
3853 CCTGCAGG
SexAI 204 ACCTGGT A^CC[AT]GGT
3507 ACCTGGT
SphI 3265 GCATGC GCATG^C
3612 GCATGC
Sse8387I 3281 CCTGCAGG CCTGCA^GG
3853 CCTGCAGG
Van91I 199 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3359 CCATCCTGTGG
XbaI 1112 TCTAGA T^CTAGA
5443 TCTAGA
XhoI 1307 CTCGAG C^TCGAG
1998 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI