##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvrII BbeI
BbrPI BbsI BcgI BciVI BclI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI CelII ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI KasI KpnI KspI MamI
MluI MroI MvnI NarI NcoI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1502 GACGTC GACGT^C
AcyI 1502 GACGTC G[GA]^CG[CT]C
AflII 2144 CTTAAG C^TTAAG
AspI 1886 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1688 TGCGCA TGC^GCA
BbvCI 2203 GCTGAGG CCTCAGC(-5/-2)
BfrI 2144 CTTAAG C^TTAAG
BsaAI 1430 TACGTG [CT]AC^GT[GA]
BsaHI 1502 GACGTC G[GA]^CG[CT]C
BsaWI 2944 TCCGGT [AT]^CCGG[AT]
BsrFI 2199 GCCGGC [GA]^CCGG[CT]
Bst1107I 4625 GTATAC GTA^TAC
BstXI 2287 CCAAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4625 GTATAC GTA^TAC
Cfr10I 2199 GCCGGC [GA]^CCGG[CT]
DsaI 1372 CCGTGG C^C[GA][CT]GG
EcoNI 1141 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1688 TGCGCA TGC^GCA
HaeII 118 AGCGCC [GA]GCGC^[CT]
HgaI 760 GACGC GACGC(5/10)
Hin1I 1502 GACGTC G[GA]^CG[CT]C
HpaI 4232 GTTAAC GTT^AAC
MfeI 1682 CAATTG C^AATTG
MunI 1682 CAATTG C^AATTG
NaeI 2199 GCCGGC GCC^GGC
NdeI 3562 CATATG CA^TATG
NgoMIV 2199 GCCGGC G^CCGGC
PaeR7I 542 CTCGAG C^TCGAG
PflFI 1886 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1317 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 785 GAGCTC GAGCT^C
SapI 782 GAAGAGC GCTCTTC(1/4)
ScaI 3427 AGTACT AGT^ACT
SpeI 3254 ACTAGT A^CTAGT
SwaI 2933 ATTTAAAT ATTT^AAAT
Tth111I 1886 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1317 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 586 TCTAGA T^CTAGA
XcmI 1202 CCAATCCAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 542 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 479 GTAGAC GT^[AC][GT]AC
4625 GTATAC
AlwNI 24 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1457 CAGAGCCTG
AocI 1478 CCTGAGG CC^T[ACGT]AGG
2628 CCTCAGG
AseI 1926 ATTAAT AT^TAAT
2318 ATTAAT
AsnI 1926 ATTAAT AT^TAAT
2318 ATTAAT
Asp700I 457 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3366 GAAATATTTC
BalI 1314 TGGCCA TGG^CCA
4124 TGGCCA
BamHI 393 GGATCC G^GATCC
2282 GGATCC
BanI 1106 GGCACC G^G[CT][GA]CC
3620 GGTGCC
BcnI 408 CCCGG CC^[GC]GG
3867 CCGGG
BsgI 1487 CTGCAC GTGCAG(16/14)
294 GTGCAG
BsmI 2653 GAATGC GAATGC(1/-1)
4419 GCATTC
BspHI 1905 TCATGA T^CATGA
3527 TCATGA
BspLU11I 906 ACATGT A^CATGT
1983 ACATGT
BstAPI 1456 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1489 GCACGCCATGC
Bsu36I 1478 CCTGAGG CC^T[ACGT]AGG
2628 CCTCAGG
EaeI 1314 TGGCCA [CT]^GGCC[GA]
4124 TGGCCA
Eco81I 1478 CCTGAGG CC^T[ACGT]AGG
2628 CCTCAGG
EcoRI 461 GAATTC G^AATTC
1286 GAATTC
EcoT22I 2612 ATGCAT ATGCA^T
2655 ATGCAT
HindIII 1782 AAGCTT A^AGCTT
3380 AAGCTT
MluNI 1314 TGGCCA TGG^CCA
4124 TGGCCA
MscI 1314 TGGCCA TGG^CCA
4124 TGGCCA
NciI 408 CCCGG CC^[GC]GG
3867 CCGGG
NsiI 2612 ATGCAT ATGCA^T
2655 ATGCAT
PpuMI 145 AGGACCT [GA]G^G[AT]CC[CT]
2632 AGGTCCT
PshBI 1926 ATTAAT AT^TAAT
2318 ATTAAT
RcaI 1905 TCATGA T^CATGA
3527 TCATGA
SphI 697 GCATGC GCATG^C
4538 GCATGC
SspI 3368 AATATT AAT^ATT
3642 AATATT
XmnI 457 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3366 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DrdI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII