##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Aor51HI AscI Asp718I AspEI BamHI
BbrPI BciVI BclI BsaBI BsaWI
BseAI BsiWI BspDI BspEI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
FbaI FseI KpnI MamI MluI
MroI NotI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SalI SfiI SfuI
SgrAI SnaBI StuI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 4004 AACGTT AA^CGTT
AflII 3076 CTTAAG C^TTAAG
Alw44I 5531 GTGCAC G^TGCAC
ApaLI 5531 GTGCAC G^TGCAC
AseI 1595 ATTAAT AT^TAAT
AsnI 1595 ATTAAT AT^TAAT
AspI 873 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3236 TGCGCA TGC^GCA
BfrI 3076 CTTAAG C^TTAAG
BglII 3926 AGATCT A^GATCT
BsaAI 4635 CACGTA [CT]AC^GT[GA]
BsiEI 5504 CGGCCG CG[GA][CT]^CG
BsrDI 4716 GCAATG GCAATG(2/0)
BsrFI 2429 GCCGGC [GA]^CCGG[CT]
BssHII 444 GCGCGC G^CGCGC
BstEII 2035 GGTGACC G^GT[ACGT]ACC
BstPI 2035 GGTGACC G^GT[ACGT]ACC
Cfr10I 2429 GCCGGC [GA]^CCGG[CT]
EagI 5504 CGGCCG C^GGCCG
EclXI 5504 CGGCCG C^GGCCG
Eco52I 5504 CGGCCG C^GGCCG
EcoO65I 2035 GGTGACC G^GT[ACGT]ACC
FspI 3236 TGCGCA TGC^GCA
HindIII 316 AAGCTT A^AGCTT
KspI 1486 CCGCGG CCGC^GG
MfeI 4275 CAATTG C^AATTG
MunI 4275 CAATTG C^AATTG
NaeI 2429 GCCGGC GCC^GGC
NgoMIV 2429 GCCGGC G^CCGGC
NheI 1300 GCTAGC G^CTAGC
NruI 86 TCGCGA TCG^CGA
PflFI 873 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1595 ATTAAT AT^TAAT
Psp1406I 4004 AACGTT AA^CGTT
SacII 1486 CCGCGG CCGC^GG
SapI 2455 GAAGAGC GCTCTTC(1/4)
SbfI 2345 CCTGCAGG CCTGCA^GG
ScaI 4152 AGTACT AGT^ACT
SmlI 3076 CTTAAG C^T[CT][GA]AG
SpeI 4071 ACTAGT A^CTAGT
Sse8387I 2345 CCTGCAGG CCTGCA^GG
Tth111I 873 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
ApaI 74 GGGCCC GGGCC^C
1369 GGGCCC
Asp700I 3108 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5412 GAAACAGTTC
AvrII 1397 CCTAGG C^CTAGG
3493 CCTAGG
BcgI 788 GCAGGAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1062 GCAGGAGAATCG
BglI 21 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2272 GCCGTAGTGGC
BlnI 1397 CCTAGG C^CTAGG
3493 CCTAGG
BlpI 738 GCTGAGC GC^T[ACGT]AGC
4394 GCTTAGC
Bpu1102I 738 GCTGAGC GC^T[ACGT]AGC
4394 GCTTAGC
BsmBI 992 CGTCTC CGTCTC(1/5)
591 GAGACG
BsmI 1721 GCATTC GAATGC(1/-1)
3651 GCATTC
Bsp1407I 2242 TGTACA T^GTACA
5142 TGTACA
BspHI 1715 TCATGA T^CATGA
4096 TCATGA
BspLU11I 3883 ACATGT A^CATGT
5299 ACATGT
BsrBI 710 CCGCTC CCGCTC(-3/-3)
2292 GAGCGG
BsrGI 2242 TGTACA T^GTACA
5142 TGTACA
CelII 738 GCTGAGC GC^T[ACGT]AGC
4394 GCTTAGC
EcoT22I 1809 ATGCAT ATGCA^T
4410 ATGCAT
HgaI 1033 GACGC GACGC(5/10)
83 GCGTC
HpaI 3532 GTTAAC GTT^AAC
4945 GTTAAC
NdeI 1902 CATATG CA^TATG
2101 CATATG
NsiI 1809 ATGCAT ATGCA^T
4410 ATGCAT
PflMI 767 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1561 CCACTTTTTGG
RcaI 1715 TCATGA T^CATGA
4096 TCATGA
SacI 2007 GAGCTC GAGCT^C
2285 GAGCTC
SexAI 3944 ACCTGGT A^CC[AT]GGT
4912 ACCTGGT
SmaI 1080 CCCGGG CCC^GGG
3345 CCCGGG
SphI 3258 GCATGC GCATG^C
3365 GCATGC
SspBI 2242 TGTACA T^GTACA
5142 TGTACA
SwaI 4795 ATTTAAAT ATTT^AAAT
4835 ATTTAAAT
Van91I 767 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1561 CCACTTTTTGG
XmaCI 1080 CCCGGG C^CCGGG
3345 CCCGGG
XmaI 1080 CCCGGG C^CCGGG
3345 CCCGGG
XmnI 3108 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5412 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII