################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccIII AclI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII BglI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HpaI KpnI KspI MamI MluI MroI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SnaBI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 138 GACGTC GACGT^C AccI 4751 GTAGAC GT^[AC][GT]AC AccII 3992 CGCG CG^CG AflIII 5375 ACATGT A^C[GA][CT]GT AgeI 4193 ACCGGT A^CCGGT AocI 4371 CCTCAGG CC^T[ACGT]AGG Asp700I 5166 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 5478 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1383 TGGCCA TGG^CCA BbeI 264 GGCGCC GGCGC^C BbrPI 4117 CACGTG CAC^GTG BbsI 2528 GTCTTC GAAGAC(2/6) BciVI 2925 GGATAC GTATCC(6/5) BclI 3345 TGATCA T^GATCA BglII 4963 AGATCT A^GATCT BpuAI 2528 GTCTTC GAAGAC(2/6) BsaAI 4117 CACGTG [CT]AC^GT[GA] BsaWI 4193 ACCGGT [AT]^CCGG[AT] BsmBI 4792 GAGACG CGTCTC(1/5) BspHI 5692 TCATGA T^CATGA BspLU11I 5375 ACATGT A^CATGT BsrBI 4099 GAGCGG CCGCTC(-3/-3) BstAPI 4223 GCACTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3313 TTCGAA TT^CGAA BstEII 3414 GGTAACC G^GT[ACGT]ACC BstPI 3414 GGTAACC G^GT[ACGT]ACC BstUI 3992 CGCG CG^CG Bsu36I 4371 CCTCAGG CC^T[ACGT]AGG DraII 1451 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 2830 GACCTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4371 CCTCAGG CC^T[ACGT]AGG EcoO109I 1451 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 3414 GGTAACC G^GT[ACGT]ACC EcoRV 3319 GATATC GAT^ATC FbaI 3345 TGATCA T^GATCA KasI 264 GGCGCC G^GCGCC MfeI 2497 CAATTG C^AATTG MluNI 1383 TGGCCA TGG^CCA MscI 1383 TGGCCA TGG^CCA MunI 2497 CAATTG C^AATTG MvnI 3992 CGCG CG^CG NarI 264 GGCGCC GG^CGCC NdeI 3409 CATATG CA^TATG NspV 3313 TTCGAA TT^CGAA PflFI 5478 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4193 ACCGGT A^CCGGT PmaCI 4117 CACGTG CAC^GTG PmlI 4117 CACGTG CAC^GTG RcaI 5692 TCATGA T^CATGA SfoI 264 GGCGCC GGC^GCC SfuI 3313 TTCGAA TT^CGAA SgrAI 4192 CACCGGTG C[GA]^CCGG[CT]G SphI 3698 GCATGC GCATG^C Tth111I 5478 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 5166 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflII 1569 CTTAAG C^TTAAG 1990 CTTAAG Alw44I 45 GTGCAC G^TGCAC 609 GTGCAC AlwNI 409 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 1243 CAGTACCTG ApaLI 45 GTGCAC G^TGCAC 609 GTGCAC AspHI 45 GTGCAC G[AT]GC[AT]^C 609 GTGCAC AvaI 5548 CCCGGG C^[CT]CG[GA]G 5751 CCCGGG AvrII 2651 CCTAGG C^CTAGG 2760 CCTAGG BamHI 484 GGATCC G^GATCC 3928 GGATCC BanII 1061 GAGCCC G[GA]GC[CT]^C 4884 GGGCTC BcgI 4074 CGAGGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 240 GCACTCCTCTCG BfrI 1569 CTTAAG C^TTAAG 1990 CTTAAG BlnI 2651 CCTAGG C^CTAGG 2760 CCTAGG BsaI 4666 GAGACC GGTCTC(1/5) 633 GGTCTC BsiHKAI 45 GTGCAC G[AT]GC[AT]^C 609 GTGCAC BsoBI 5548 CCCGGG C^[CT]CG[GA]G 5751 CCCGGG BsrFI 3955 GCCGGT [GA]^CCGG[CT] 4193 ACCGGT BssSI 16 CTCGTG CACGAG(-5/-1) 5009 CTCGTG Cfr10I 3955 GCCGGT [GA]^CCGG[CT] 4193 ACCGGT EaeI 1383 TGGCCA [CT]^GGCC[GA] 4121 TGGCCG EcoNI 1512 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3813 CCTGTGACAGG EcoRI 2283 GAATTC G^AATTC 4186 GAATTC FauI 5604 CCCGC CCCGC(4/6) 4101 GCGGG HaeII 264 GGCGCC [GA]GCGC^[CT] 4848 AGCGCC HgaI 4794 GACGC GACGC(5/10) 2322 GCGTC SapI 4526 GAAGAGC GCTCTTC(1/4) 3776 GCTCTTC SmaI 5548 CCCGGG CCC^GGG 5751 CCCGGG SpeI 1100 ACTAGT A^CTAGT 1575 ACTAGT SspI 1402 AATATT AAT^ATT 3473 AATATT XmaCI 5548 CCCGGG C^CCGGG 5751 CCCGGG XmaI 5548 CCCGGG C^CCGGG 5751 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AseI AsnI AvaII BanI BbvCI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII DsaI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII