##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
BglI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HpaI KpnI KspI MamI MluI
MroI NaeI NgoMIV NheI NotI
NruI PacI PaeR7I PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SnaBI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 138 GACGTC GACGT^C
AccI 4751 GTAGAC GT^[AC][GT]AC
AccII 3992 CGCG CG^CG
AflIII 5375 ACATGT A^C[GA][CT]GT
AgeI 4193 ACCGGT A^CCGGT
AocI 4371 CCTCAGG CC^T[ACGT]AGG
Asp700I 5166 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5478 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1383 TGGCCA TGG^CCA
BbeI 264 GGCGCC GGCGC^C
BbrPI 4117 CACGTG CAC^GTG
BbsI 2528 GTCTTC GAAGAC(2/6)
BciVI 2925 GGATAC GTATCC(6/5)
BclI 3345 TGATCA T^GATCA
BglII 4963 AGATCT A^GATCT
BpuAI 2528 GTCTTC GAAGAC(2/6)
BsaAI 4117 CACGTG [CT]AC^GT[GA]
BsaWI 4193 ACCGGT [AT]^CCGG[AT]
BsmBI 4792 GAGACG CGTCTC(1/5)
BspHI 5692 TCATGA T^CATGA
BspLU11I 5375 ACATGT A^CATGT
BsrBI 4099 GAGCGG CCGCTC(-3/-3)
BstAPI 4223 GCACTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3313 TTCGAA TT^CGAA
BstEII 3414 GGTAACC G^GT[ACGT]ACC
BstPI 3414 GGTAACC G^GT[ACGT]ACC
BstUI 3992 CGCG CG^CG
Bsu36I 4371 CCTCAGG CC^T[ACGT]AGG
DraII 1451 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 2830 GACCTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4371 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1451 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3414 GGTAACC G^GT[ACGT]ACC
EcoRV 3319 GATATC GAT^ATC
FbaI 3345 TGATCA T^GATCA
KasI 264 GGCGCC G^GCGCC
MfeI 2497 CAATTG C^AATTG
MluNI 1383 TGGCCA TGG^CCA
MscI 1383 TGGCCA TGG^CCA
MunI 2497 CAATTG C^AATTG
MvnI 3992 CGCG CG^CG
NarI 264 GGCGCC GG^CGCC
NdeI 3409 CATATG CA^TATG
NspV 3313 TTCGAA TT^CGAA
PflFI 5478 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4193 ACCGGT A^CCGGT
PmaCI 4117 CACGTG CAC^GTG
PmlI 4117 CACGTG CAC^GTG
RcaI 5692 TCATGA T^CATGA
SfoI 264 GGCGCC GGC^GCC
SfuI 3313 TTCGAA TT^CGAA
SgrAI 4192 CACCGGTG C[GA]^CCGG[CT]G
SphI 3698 GCATGC GCATG^C
Tth111I 5478 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5166 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 1569 CTTAAG C^TTAAG
1990 CTTAAG
Alw44I 45 GTGCAC G^TGCAC
609 GTGCAC
AlwNI 409 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1243 CAGTACCTG
ApaLI 45 GTGCAC G^TGCAC
609 GTGCAC
AspHI 45 GTGCAC G[AT]GC[AT]^C
609 GTGCAC
AvaI 5548 CCCGGG C^[CT]CG[GA]G
5751 CCCGGG
AvrII 2651 CCTAGG C^CTAGG
2760 CCTAGG
BamHI 484 GGATCC G^GATCC
3928 GGATCC
BanII 1061 GAGCCC G[GA]GC[CT]^C
4884 GGGCTC
BcgI 4074 CGAGGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
240 GCACTCCTCTCG
BfrI 1569 CTTAAG C^TTAAG
1990 CTTAAG
BlnI 2651 CCTAGG C^CTAGG
2760 CCTAGG
BsaI 4666 GAGACC GGTCTC(1/5)
633 GGTCTC
BsiHKAI 45 GTGCAC G[AT]GC[AT]^C
609 GTGCAC
BsoBI 5548 CCCGGG C^[CT]CG[GA]G
5751 CCCGGG
BsrFI 3955 GCCGGT [GA]^CCGG[CT]
4193 ACCGGT
BssSI 16 CTCGTG CACGAG(-5/-1)
5009 CTCGTG
Cfr10I 3955 GCCGGT [GA]^CCGG[CT]
4193 ACCGGT
EaeI 1383 TGGCCA [CT]^GGCC[GA]
4121 TGGCCG
EcoNI 1512 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3813 CCTGTGACAGG
EcoRI 2283 GAATTC G^AATTC
4186 GAATTC
FauI 5604 CCCGC CCCGC(4/6)
4101 GCGGG
HaeII 264 GGCGCC [GA]GCGC^[CT]
4848 AGCGCC
HgaI 4794 GACGC GACGC(5/10)
2322 GCGTC
SapI 4526 GAAGAGC GCTCTTC(1/4)
3776 GCTCTTC
SmaI 5548 CCCGGG CCC^GGG
5751 CCCGGG
SpeI 1100 ACTAGT A^CTAGT
1575 ACTAGT
SspI 1402 AATATT AAT^ATT
3473 AATATT
XmaCI 5548 CCCGGG C^CCGGG
5751 CCCGGG
XmaI 5548 CCCGGG C^CCGGG
5751 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BanI BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DsaI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII