################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AccIII AclI AgeI Aor51HI AscI Asp718I AspI BbeI BciVI BclI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII BstBI ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FauI FbaI FseI HindIII KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2929 GACGTC GACGT^C AccII 1952 CGCG CG^CG AcyI 2929 GACGTC G[GA]^CG[CT]C AhdI 4674 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 19 GGGCCC GGGCC^C AspEI 4674 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 5085 TGCGCA TGC^GCA BanI 4751 GGTGCC G^G[CT][GA]CC BbrPI 2752 CACGTG CAC^GTG BcgI 879 GCATTCAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 2929 GACGTC G[GA]^CG[CT]C BsiEI 763 CGGTCG CG[GA][CT]^CG BspHI 4633 TCATGA T^CATGA BspLU11I 3291 ACATGT A^CATGT BsrFI 1068 GCCGGT [GA]^CCGG[CT] Bst1107I 5201 GTATAC GTA^TAC BstEII 4836 GGTGACC G^GT[ACGT]ACC BstPI 4836 GGTGACC G^GT[ACGT]ACC BstUI 1952 CGCG CG^CG BstZ17I 5201 GTATAC GTA^TAC Cfr10I 1068 GCCGGT [GA]^CCGG[CT] DraIII 4278 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 5544 GACACCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 4674 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4836 GGTGACC G^GT[ACGT]ACC EcoRI 3072 GAATTC G^AATTC FspI 5085 TGCGCA TGC^GCA HaeII 2498 AGCGCC [GA]GCGC^[CT] HgaI 90 GACGC GACGC(5/10) Hin1I 2929 GACGTC G[GA]^CG[CT]C HincII 5234 GTTAAC GT[CT]^[GA]AC HindII 5234 GTTAAC GT[CT]^[GA]AC HpaI 5234 GTTAAC GTT^AAC MvnI 1952 CGCG CG^CG PaeR7I 860 CTCGAG C^TCGAG PflMI 1674 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2752 CACGTG CAC^GTG PmlI 2752 CACGTG CAC^GTG RcaI 4633 TCATGA T^CATGA SmaI 4647 CCCGGG CCC^GGG SspI 5577 AATATT AAT^ATT StuI 328 AGGCCT AGG^CCT Van91I 1674 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 860 CTCGAG C^TCGAG XmaCI 4647 CCCGGG C^CCGGG XmaI 4647 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 3291 ACATGT A^C[GA][CT]GT 3633 ACACGT AlwNI 176 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 5401 CAGGTACTG AseI 2016 ATTAAT AT^TAAT 2059 ATTAAT AsnI 2016 ATTAAT AT^TAAT 2059 ATTAAT Asp700I 2630 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3014 GAAGTCCTTC AvrII 749 CCTAGG C^CTAGG 853 CCTAGG BamHI 1683 GGATCC G^GATCC 4444 GGATCC BlnI 749 CCTAGG C^CTAGG 853 CCTAGG BsaAI 2725 TACGTG [CT]AC^GT[GA] 2752 CACGTG BsaBI 2471 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3647 GATGCCAATC BsmBI 3418 CGTCTC CGTCTC(1/5) 88 GAGACG BspMI 1504 GCAGGT ACCTGC(4/8) 4069 GCAGGT BssSI 596 CACGAG CACGAG(-5/-1) 5386 CACGAG BstAPI 1928 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2865 GCACTGGCTGC MamI 2471 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3647 GATGCCAATC NheI 5431 GCTAGC G^CTAGC 5435 GCTAGC PshAI 851 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3895 GACCACAGTC PshBI 2016 ATTAAT AT^TAAT 2059 ATTAAT SapI 4314 GAAGAGC GCTCTTC(1/4) 4560 GAAGAGC XcmI 1317 CCAAGCTGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3922 CCAGCCCAGCCTTGG XmnI 2630 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3014 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI Alw44I AlwI AocI ApaLI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII