##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I AspI BbeI BciVI
BclI BsaWI BseAI BsiWI BspDI
BspEI BsrBI BssHII BstBI ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FauI FbaI FseI
HindIII KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2929 GACGTC GACGT^C
AccII 1952 CGCG CG^CG
AcyI 2929 GACGTC G[GA]^CG[CT]C
AhdI 4674 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 19 GGGCCC GGGCC^C
AspEI 4674 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5085 TGCGCA TGC^GCA
BanI 4751 GGTGCC G^G[CT][GA]CC
BbrPI 2752 CACGTG CAC^GTG
BcgI 879 GCATTCAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 2929 GACGTC G[GA]^CG[CT]C
BsiEI 763 CGGTCG CG[GA][CT]^CG
BspHI 4633 TCATGA T^CATGA
BspLU11I 3291 ACATGT A^CATGT
BsrFI 1068 GCCGGT [GA]^CCGG[CT]
Bst1107I 5201 GTATAC GTA^TAC
BstEII 4836 GGTGACC G^GT[ACGT]ACC
BstPI 4836 GGTGACC G^GT[ACGT]ACC
BstUI 1952 CGCG CG^CG
BstZ17I 5201 GTATAC GTA^TAC
Cfr10I 1068 GCCGGT [GA]^CCGG[CT]
DraIII 4278 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5544 GACACCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4674 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4836 GGTGACC G^GT[ACGT]ACC
EcoRI 3072 GAATTC G^AATTC
FspI 5085 TGCGCA TGC^GCA
HaeII 2498 AGCGCC [GA]GCGC^[CT]
HgaI 90 GACGC GACGC(5/10)
Hin1I 2929 GACGTC G[GA]^CG[CT]C
HincII 5234 GTTAAC GT[CT]^[GA]AC
HindII 5234 GTTAAC GT[CT]^[GA]AC
HpaI 5234 GTTAAC GTT^AAC
MvnI 1952 CGCG CG^CG
PaeR7I 860 CTCGAG C^TCGAG
PflMI 1674 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2752 CACGTG CAC^GTG
PmlI 2752 CACGTG CAC^GTG
RcaI 4633 TCATGA T^CATGA
SmaI 4647 CCCGGG CCC^GGG
SspI 5577 AATATT AAT^ATT
StuI 328 AGGCCT AGG^CCT
Van91I 1674 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 860 CTCGAG C^TCGAG
XmaCI 4647 CCCGGG C^CCGGG
XmaI 4647 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 3291 ACATGT A^C[GA][CT]GT
3633 ACACGT
AlwNI 176 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
5401 CAGGTACTG
AseI 2016 ATTAAT AT^TAAT
2059 ATTAAT
AsnI 2016 ATTAAT AT^TAAT
2059 ATTAAT
Asp700I 2630 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3014 GAAGTCCTTC
AvrII 749 CCTAGG C^CTAGG
853 CCTAGG
BamHI 1683 GGATCC G^GATCC
4444 GGATCC
BlnI 749 CCTAGG C^CTAGG
853 CCTAGG
BsaAI 2725 TACGTG [CT]AC^GT[GA]
2752 CACGTG
BsaBI 2471 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3647 GATGCCAATC
BsmBI 3418 CGTCTC CGTCTC(1/5)
88 GAGACG
BspMI 1504 GCAGGT ACCTGC(4/8)
4069 GCAGGT
BssSI 596 CACGAG CACGAG(-5/-1)
5386 CACGAG
BstAPI 1928 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2865 GCACTGGCTGC
MamI 2471 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3647 GATGCCAATC
NheI 5431 GCTAGC G^CTAGC
5435 GCTAGC
PshAI 851 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3895 GACCACAGTC
PshBI 2016 ATTAAT AT^TAAT
2059 ATTAAT
SapI 4314 GAAGAGC GCTCTTC(1/4)
4560 GAAGAGC
XcmI 1317 CCAAGCTGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3922 CCAGCCCAGCCTTGG
XmnI 2630 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3014 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI Alw44I AlwI AocI ApaLI
ApoI AspHI AvaI AvaII BalI
BanII BbsI BbvCI BbvI BcnI
BfaI BfrI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII