##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
Aor51HI AscI Asp700I AspEI AviII
BbeI BbrPI BcgI BciVI BfrI
BseAI BsiWI BspDI BspEI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoT22I FseI FspI HaeII KasI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4398 GGTACC G^GTACC
AclI 4805 AACGTT AA^CGTT
Alw44I 3475 GTGCAC G^TGCAC
ApaLI 3475 GTGCAC G^TGCAC
AseI 2669 ATTAAT AT^TAAT
AsnI 2669 ATTAAT AT^TAAT
Asp718I 4398 GGTACC G^GTACC
AvrII 1346 CCTAGG C^CTAGG
BalI 4630 TGGCCA TGG^CCA
BamHI 1620 GGATCC G^GATCC
BglI 4882 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2714 AGATCT A^GATCT
BlnI 1346 CCTAGG C^CTAGG
BsaAI 2389 CACGTA [CT]AC^GT[GA]
BsaBI 1573 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 518 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2450 TGTACA T^GTACA
BspHI 3013 TCATGA T^CATGA
BsrGI 2450 TGTACA T^GTACA
DraIII 4707 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4131 CGGCCG C^GGCCG
EclXI 4131 CGGCCG C^GGCCG
Eco52I 4131 CGGCCG C^GGCCG
EcoRI 2896 GAATTC G^AATTC
HincII 995 GTTAAC GT[CT]^[GA]AC
HindII 995 GTTAAC GT[CT]^[GA]AC
HpaI 995 GTTAAC GTT^AAC
KpnI 4398 GGTACC GGTAC^C
MamI 1573 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4630 TGGCCA TGG^CCA
MscI 4630 TGGCCA TGG^CCA
PflMI 1142 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2669 ATTAAT AT^TAAT
Psp1406I 4805 AACGTT AA^CGTT
PstI 5015 CTGCAG CTGCA^G
RcaI 3013 TCATGA T^CATGA
SphI 4613 GCATGC GCATG^C
SspBI 2450 TGTACA T^GTACA
SspI 4739 AATATT AAT^ATT
Van91I 1142 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1057 GACGTC GACGT^C
2208 GACGTC
AocI 3605 CCTGAGG CC^T[ACGT]AGG
4066 CCTCAGG
ApaI 5298 GGGCCC GGGCC^C
5342 GGGCCC
AspI 2883 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5054 GACTCTGTC
AvaI 5401 CTCGGG C^[CT]CG[GA]G
5612 CTCGGG
BclI 2410 TGATCA T^GATCA
2438 TGATCA
BlpI 1926 GCTGAGC GC^T[ACGT]AGC
3316 GCTCAGC
Bpu1102I 1926 GCTGAGC GC^T[ACGT]AGC
3316 GCTCAGC
BsiEI 88 CGGTCG CG[GA][CT]^CG
4131 CGGCCG
BsmBI 2970 CGTCTC CGTCTC(1/5)
3856 GAGACG
BsmI 1274 GAATGC GAATGC(1/-1)
3057 GAATGC
BsoBI 5401 CTCGGG C^[CT]CG[GA]G
5612 CTCGGG
BspLU11I 2488 ACATGT A^CATGT
4604 ACATGT
BsrBI 67 GAGCGG CCGCTC(-3/-3)
321 GAGCGG
Bsu36I 3605 CCTGAGG CC^T[ACGT]AGG
4066 CCTCAGG
CelII 1926 GCTGAGC GC^T[ACGT]AGC
3316 GCTCAGC
DrdI 2286 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2962 GACTTCCGCGTC
Eco81I 3605 CCTGAGG CC^T[ACGT]AGG
4066 CCTCAGG
FbaI 2410 TGATCA T^GATCA
2438 TGATCA
HindIII 271 AAGCTT A^AGCTT
703 AAGCTT
MfeI 502 CAATTG C^AATTG
4991 CAATTG
MunI 502 CAATTG C^AATTG
4991 CAATTG
NcoI 1142 CCATGG C^CATGG
2891 CCATGG
PflFI 2883 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5054 GACTCTGTC
SacI 2542 GAGCTC GAGCT^C
4872 GAGCTC
SapI 2733 GAAGAGC GCTCTTC(1/4)
4648 GAAGAGC
StuI 1686 AGGCCT AGG^CCT
4942 AGGCCT
Tth111I 2883 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5054 GACTCTGTC
XcmI 4187 CCACAAACACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4441 CCAGGTGGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshAI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII