##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AclI AgeI AhdI AscI AspEI
AviII AvrII BbeI BglI BlnI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoT22I FseI FspI
HindIII HpaI KasI KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PflMI PinAI
PmeI Psp1406I PvuI RsrII SacII
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 994 GACGTC GACGT^C
Acc65I 3036 GGTACC G^GTACC
AccII 20 CGCG CG^CG
Alw44I 4070 GTGCAC G^TGCAC
Aor51HI 4659 AGCGCT AGC^GCT
ApaLI 4070 GTGCAC G^TGCAC
AseI 376 ATTAAT AT^TAAT
AsnI 376 ATTAAT AT^TAAT
Asp718I 3036 GGTACC G^GTACC
BamHI 4027 GGATCC G^GATCC
BbsI 3886 GTCTTC GAAGAC(2/6)
BcgI 2336 CGAAACTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1240 AGATCT A^GATCT
BpuAI 3886 GTCTTC GAAGAC(2/6)
BspHI 2320 TCATGA T^CATGA
BssSI 4260 CTCGTG CACGAG(-5/-1)
BstUI 20 CGCG CG^CG
Eco47III 4659 AGCGCT AGC^GCT
EcoRI 2594 GAATTC G^AATTC
EcoRV 2944 GATATC GAT^ATC
KpnI 3036 GGTACC GGTAC^C
MvnI 20 CGCG CG^CG
NcoI 1060 CCATGG C^CATGG
PacI 373 TTAATTAA TTAAT^TAA
PpuMI 3023 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3452 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 376 ATTAAT AT^TAAT
RcaI 2320 TCATGA T^CATGA
SacI 2160 GAGCTC GAGCT^C
SalI 1173 GTCGAC G^TCGAC
SapI 3833 GCTCTTC GCTCTTC(1/4)
SbfI 3280 CCTGCAGG CCTGCA^GG
SphI 1315 GCATGC GCATG^C
Sse8387I 3280 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 1012 GGGCCC GGGCC^C
1284 GGGCCC
AvaI 850 CCCGGG C^[CT]CG[GA]G
1003 CCCGGG
BbrPI 1017 CACGTG CAC^GTG
1110 CACGTG
BciVI 1558 GGATAC GTATCC(6/5)
1643 GGATAC
BclI 3987 TGATCA T^GATCA
4995 TGATCA
BlpI 2178 GCTGAGC GC^T[ACGT]AGC
4545 GCTGAGC
Bpu1102I 2178 GCTGAGC GC^T[ACGT]AGC
4545 GCTGAGC
BsaAI 1017 CACGTG [CT]AC^GT[GA]
1110 CACGTG
BsoBI 850 CCCGGG C^[CT]CG[GA]G
1003 CCCGGG
BstEII 1662 GGTGACC G^GT[ACGT]ACC
3096 GGTAACC
BstPI 1662 GGTGACC G^GT[ACGT]ACC
3096 GGTAACC
CelII 2178 GCTGAGC GC^T[ACGT]AGC
4545 GCTGAGC
DraI 4095 TTTAAA TTT^AAA
4235 TTTAAA
EcoO65I 1662 GGTGACC G^GT[ACGT]ACC
3096 GGTAACC
FauI 478 CCCGC CCCGC(4/6)
1095 GCGGG
FbaI 3987 TGATCA T^GATCA
4995 TGATCA
HaeII 4629 GGCGCT [GA]GCGC^[CT]
4659 AGCGCT
PmaCI 1017 CACGTG CAC^GTG
1110 CACGTG
PmlI 1017 CACGTG CAC^GTG
1110 CACGTG
SmaI 850 CCCGGG CCC^GGG
1003 CCCGGG
TaqI 1174 TCGA T^CGA
2335 TCGA
TthHB8I 1174 TCGA T^CGA
2335 TCGA
XmaCI 850 CCCGGG C^CCGGG
1003 CCCGGG
XmaI 850 CCCGGG C^CCGGG
1003 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccIII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AspI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BfrI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspEI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I XcmI XhoII
XmnI