##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbsI BbvCI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MaeI
MamI MfeI MluI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 433 GGTACC G^GTACC
AccIII 43 TCCGGA T^CCGGA
AlwI 53 GATCC GGATC(4/5)
AocI 322 CCTCAGG CC^T[ACGT]AGG
Asp718I 433 GGTACC G^GTACC
AspHI 780 GAGCTC G[AT]GC[AT]^C
BbeI 606 GGCGCC GGCGC^C
BbrPI 746 CACGTG CAC^GTG
BcgI 683 CGACATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 488 GGATAC GTATCC(6/5)
BpmI 12 CTGGAG CTGGAG(16/14)
BsaAI 746 CACGTG [CT]AC^GT[GA]
BsaWI 43 TCCGGA [AT]^CCGG[AT]
BseAI 43 TCCGGA T^CCGGA
BsgI 913 GTGCAG GTGCAG(16/14)
BsiEI 908 CGACCG CG[GA][CT]^CG
BsiHKAI 780 GAGCTC G[AT]GC[AT]^C
BspEI 43 TCCGGA T^CCGGA
BspMI 763 ACCTGC ACCTGC(4/8)
BsrFI 729 ACCGGC [GA]^CCGG[CT]
BstEII 959 GGTCACC G^GT[ACGT]ACC
BstPI 959 GGTCACC G^GT[ACGT]ACC
Bsu36I 322 CCTCAGG CC^T[ACGT]AGG
Cfr10I 729 ACCGGC [GA]^CCGG[CT]
EarI 756 CTCTTC CTCTTC(1/4)
Eco81I 322 CCTCAGG CC^T[ACGT]AGG
EcoO65I 959 GGTCACC G^GT[ACGT]ACC
EcoT14I 1136 CCTTGG C^C[AT][AT]GG
HgaI 741 GACGC GACGC(5/10)
KasI 606 GGCGCC G^GCGCC
KpnI 433 GGTACC GGTAC^C
Ksp632I 756 CTCTTC CTCTTC(1/4)
MaeIII 960 GTCAC ^GT[ACGT]AC
MroI 43 TCCGGA T^CCGGA
NarI 606 GGCGCC GG^CGCC
NspI 72 GCATGT [GA]CATG^[CT]
PflMI 181 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 746 CACGTG CAC^GTG
PmlI 746 CACGTG CAC^GTG
PvuII 103 CAGCTG CAG^CTG
SacI 780 GAGCTC GAGCT^C
SfoI 606 GGCGCC GGC^GCC
SmaI 1122 CCCGGG CCC^GGG
StyI 1136 CCTTGG C^C[AT][AT]GG
TaqI 760 TCGA T^CGA
Tsp45I 960 GTCAC ^GT[GC]AC
TthHB8I 760 TCGA T^CGA
Van91I 181 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1122 CCCGGG C^CCGGG
XmaI 1122 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 606 GGCGCC G[GA]^CG[CT]C
741 GACGCC
AfaI 434 GTAC GT^AC
791 GTAC
ApaI 1042 GGGCCC GGGCC^C
1058 GGGCCC
AvaI 954 CTCGGG C^[CT]CG[GA]G
1122 CCCGGG
BglII 172 AGATCT A^GATCT
1012 AGATCT
BsaHI 606 GGCGCC G[GA]^CG[CT]C
741 GACGCC
BseRI 290 CTCCTC GAGGAG(10/8)
783 CTCCTC
BsmAI 23 GAGAC GTCTC(1/5)
516 GAGAC
BsoBI 954 CTCGGG C^[CT]CG[GA]G
1122 CCCGGG
DdeI 21 CTGAG C^T[ACGT]AG
323 CTCAG
HaeII 216 AGCGCC [GA]GCGC^[CT]
606 GGCGCC
Hin1I 606 GGCGCC G[GA]^CG[CT]C
741 GACGCC
MboII 703 TCTTC GAAGA(8/7)
757 TCTTC
MslI 593 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
974 CATGGCTGTG
RsaI 434 GTAC GT^AC
791 GTAC
SfcI 371 CTACAG C^T[GA][CT]AG
1078 CTACAG
XcmI 1 CCATGAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
95 CCAGATCACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AvaII BalI
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI TseI
TspRI XhoII