################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AccIII AclI AgeI Aor51HI AscI AseI AsnI BbrPI BcgI BciVI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI HpaI MamI MfeI MluI MroI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AatII 1777 GACGTC GACGT^C Acc65I 4431 GGTACC G^GTACC AflIII 3389 ACACGT A^C[GA][CT]GT Alw44I 663 GTGCAC G^TGCAC ApaLI 663 GTGCAC G^TGCAC Asp700I 4505 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4431 GGTACC G^GTACC AviII 3836 TGCGCA TGC^GCA BbeI 1715 GGCGCC GGCGC^C BstAPI 1303 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 1970 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 3836 TGCGCA TGC^GCA HindIII 1593 AAGCTT A^AGCTT KasI 1715 GGCGCC G^GCGCC KpnI 4431 GGTACC GGTAC^C KspI 1522 CCGCGG CCGC^GG NarI 1715 GGCGCC GG^CGCC NspI 2291 GCATGT [GA]CATG^[CT] SacI 3633 GAGCTC GAGCT^C SacII 1522 CCGCGG CCGC^GG SbfI 3251 CCTGCAGG CCTGCA^GG SfoI 1715 GGCGCC GGC^GCC Sse8387I 3251 CCTGCAGG CCTGCA^GG XmnI 4505 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AhdI 1772 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3469 GACAGAGTGTC AspEI 1772 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3469 GACAGAGTGTC BamHI 647 GGATCC G^GATCC 2808 GGATCC BbsI 928 GAAGAC GAAGAC(2/6) 3505 GAAGAC BclI 2385 TGATCA T^GATCA 2657 TGATCA BlpI 563 GCTCAGC GC^T[ACGT]AGC 3481 GCTCAGC Bpu1102I 563 GCTCAGC GC^T[ACGT]AGC 3481 GCTCAGC BpuAI 928 GAAGAC GAAGAC(2/6) 3505 GAAGAC BsaAI 2478 TACGTG [CT]AC^GT[GA] 3390 CACGTA BsaWI 1095 ACCGGA [AT]^CCGG[AT] 4317 TCCGGT BsmI 1751 GAATGC GAATGC(1/-1) 3139 GAATGC BsrBI 3829 GAGCGG CCGCTC(-3/-3) 4054 GAGCGG BsrFI 1637 ACCGGC [GA]^CCGG[CT] 1971 ACCGGC BstEII 1001 GGTCACC G^GT[ACGT]ACC 4045 GGTCACC BstPI 1001 GGTCACC G^GT[ACGT]ACC 4045 GGTCACC CelII 563 GCTCAGC GC^T[ACGT]AGC 3481 GCTCAGC Cfr10I 1637 ACCGGC [GA]^CCGG[CT] 1971 ACCGGC Eam1105I 1772 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3469 GACAGAGTGTC EcoO65I 1001 GGTCACC G^GT[ACGT]ACC 4045 GGTCACC FbaI 2385 TGATCA T^GATCA 2657 TGATCA HincII 1578 GTCAAC GT[CT]^[GA]AC 1888 GTTGAC HindII 1578 GTCAAC GT[CT]^[GA]AC 1888 GTTGAC NcoI 1547 CCATGG C^CATGG 3228 CCATGG PstI 1654 CTGCAG CTGCA^G 3252 CTGCAG SfiI 1030 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3786 GGCCCCGGTGGCC SspI 826 AATATT AAT^ATT 2518 AATATT StuI 3022 AGGCCT AGG^CCT 3946 AGGCCT XcmI 3186 CCATACAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3782 CCAAGGCCCCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII AvrII BalI BanI BanII BbvCI BbvI BcnI BfaI BfrI BglI BglII BlnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmaCI XmaI