##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AseI AsnI BbrPI BcgI BciVI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI HpaI MamI MfeI
MluI MroI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1777 GACGTC GACGT^C
Acc65I 4431 GGTACC G^GTACC
AflIII 3389 ACACGT A^C[GA][CT]GT
Alw44I 663 GTGCAC G^TGCAC
ApaLI 663 GTGCAC G^TGCAC
Asp700I 4505 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4431 GGTACC G^GTACC
AviII 3836 TGCGCA TGC^GCA
BbeI 1715 GGCGCC GGCGC^C
BstAPI 1303 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1970 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3836 TGCGCA TGC^GCA
HindIII 1593 AAGCTT A^AGCTT
KasI 1715 GGCGCC G^GCGCC
KpnI 4431 GGTACC GGTAC^C
KspI 1522 CCGCGG CCGC^GG
NarI 1715 GGCGCC GG^CGCC
NspI 2291 GCATGT [GA]CATG^[CT]
SacI 3633 GAGCTC GAGCT^C
SacII 1522 CCGCGG CCGC^GG
SbfI 3251 CCTGCAGG CCTGCA^GG
SfoI 1715 GGCGCC GGC^GCC
Sse8387I 3251 CCTGCAGG CCTGCA^GG
XmnI 4505 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 1772 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3469 GACAGAGTGTC
AspEI 1772 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3469 GACAGAGTGTC
BamHI 647 GGATCC G^GATCC
2808 GGATCC
BbsI 928 GAAGAC GAAGAC(2/6)
3505 GAAGAC
BclI 2385 TGATCA T^GATCA
2657 TGATCA
BlpI 563 GCTCAGC GC^T[ACGT]AGC
3481 GCTCAGC
Bpu1102I 563 GCTCAGC GC^T[ACGT]AGC
3481 GCTCAGC
BpuAI 928 GAAGAC GAAGAC(2/6)
3505 GAAGAC
BsaAI 2478 TACGTG [CT]AC^GT[GA]
3390 CACGTA
BsaWI 1095 ACCGGA [AT]^CCGG[AT]
4317 TCCGGT
BsmI 1751 GAATGC GAATGC(1/-1)
3139 GAATGC
BsrBI 3829 GAGCGG CCGCTC(-3/-3)
4054 GAGCGG
BsrFI 1637 ACCGGC [GA]^CCGG[CT]
1971 ACCGGC
BstEII 1001 GGTCACC G^GT[ACGT]ACC
4045 GGTCACC
BstPI 1001 GGTCACC G^GT[ACGT]ACC
4045 GGTCACC
CelII 563 GCTCAGC GC^T[ACGT]AGC
3481 GCTCAGC
Cfr10I 1637 ACCGGC [GA]^CCGG[CT]
1971 ACCGGC
Eam1105I 1772 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3469 GACAGAGTGTC
EcoO65I 1001 GGTCACC G^GT[ACGT]ACC
4045 GGTCACC
FbaI 2385 TGATCA T^GATCA
2657 TGATCA
HincII 1578 GTCAAC GT[CT]^[GA]AC
1888 GTTGAC
HindII 1578 GTCAAC GT[CT]^[GA]AC
1888 GTTGAC
NcoI 1547 CCATGG C^CATGG
3228 CCATGG
PstI 1654 CTGCAG CTGCA^G
3252 CTGCAG
SfiI 1030 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3786 GGCCCCGGTGGCC
SspI 826 AATATT AAT^ATT
2518 AATATT
StuI 3022 AGGCCT AGG^CCT
3946 AGGCCT
XcmI 3186 CCATACAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3782 CCAAGGCCCCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AspI
AvaI AvaII AvrII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BfrI BglI BglII BlnI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI