##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BlnI BsaAI BseAI
BsiWI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BssHII BstBI BstEII
BstPI ClaI DraIII EagI EclXI
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI HindIII KasI KpnI KspI
MluI MroI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI RcaI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 79 GACGTC GACGT^C
AccI 4507 GTATAC GT^[AC][GT]AC
AclI 4477 AACGTT AA^CGTT
AcyI 79 GACGTC G[GA]^CG[CT]C
AflII 4425 CTTAAG C^TTAAG
AflIII 1338 ACACGT A^C[GA][CT]GT
AhdI 3581 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3785 AGCGCT AGC^GCT
ApaI 3353 GGGCCC GGGCC^C
Asp700I 4391 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3581 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2243 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 64 GTATCC GTATCC(6/5)
BcnI 2914 CCGGG CC^[GC]GG
BfrI 4425 CTTAAG C^TTAAG
BsaBI 1940 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 79 GACGTC G[GA]^CG[CT]C
BsaWI 1173 TCCGGT [AT]^CCGG[AT]
BsiEI 573 CGATCG CG[GA][CT]^CG
Bsp1407I 655 TGTACA T^GTACA
BsrDI 4230 CATTGC GCAATG(2/0)
BsrGI 655 TGTACA T^GTACA
Bst1107I 4507 GTATAC GTA^TAC
BstZ17I 4507 GTATAC GTA^TAC
CpoI 1170 CGGTCCG CG^G[AT]CCG
DrdI 290 GACCGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3581 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3785 AGCGCT AGC^GCT
Hin1I 79 GACGTC G[GA]^CG[CT]C
HpaI 4271 GTTAAC GTT^AAC
MamI 1940 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3635 CAATTG C^AATTG
MunI 3635 CAATTG C^AATTG
NaeI 3283 GCCGGC GCC^GGC
NciI 2914 CCGGG CC^[GC]GG
NdeI 2036 CATATG CA^TATG
NgoMIV 3283 GCCGGC G^CCGGC
NspI 2120 ACATGC [GA]CATG^[CT]
PflFI 2243 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4477 AACGTT AA^CGTT
PvuI 573 CGATCG CGAT^CG
RsrII 1170 CGGTCCG CG^G[AT]CCG
ScaI 3623 AGTACT AGT^ACT
SpeI 2141 ACTAGT A^CTAGT
SspBI 655 TGTACA T^GTACA
SspI 4370 AATATT AAT^ATT
Tth111I 2243 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1745 TCTAGA T^CTAGA
XmnI 4391 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BclI 1827 TGATCA T^GATCA
3834 TGATCA
BglI 384 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2875 GCCAGACAGGC
BglII 1978 AGATCT A^GATCT
3985 AGATCT
BsaI 2060 GAGACC GGTCTC(1/5)
2832 GAGACC
BsmBI 1785 CGTCTC CGTCTC(1/5)
2837 CGTCTC
BsmI 3526 GCATTC GAATGC(1/-1)
4234 GCATTC
BssSI 589 CACGAG CACGAG(-5/-1)
1160 CACGAG
BstXI 423 CCATAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3502 CCATGGTAGTGG
DraI 664 TTTAAA TTT^AAA
3250 TTTAAA
EcoRV 764 GATATC GAT^ATC
3594 GATATC
FbaI 1827 TGATCA T^GATCA
3834 TGATCA
HaeII 2925 GGCGCT [GA]GCGC^[CT]
3785 AGCGCT
NcoI 343 CCATGG C^CATGG
3502 CCATGG
PpuMI 995 GGGTCCT [GA]G^G[AT]CC[CT]
3809 AGGTCCC
SacI 275 GAGCTC GAGCT^C
1851 GAGCTC
SapI 2980 GAAGAGC GCTCTTC(1/4)
1291 GCTCTTC
TaqI 1302 TCGA T^CGA
1840 TCGA
TthHB8I 1302 TCGA T^CGA
1840 TCGA
XcmI 3493 CCAGGTCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3499 CCACCATGGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII