##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AcyI AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BbeI BbvCI BclI BlnI BsaBI
BsaHI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI Eco47III EcoRI
EcoT22I FbaI FspI HgaI Hin1I
HpaI KasI MamI MluI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfoI SgrAI SnaBI SpeI
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1811 CGCG CG^CG
AccIII 1635 TCCGGA T^CCGGA
AcsI 4394 AAATTC [GA]^AATT[CT]
AflII 2478 CTTAAG C^TTAAG
AhdI 126 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4394 AAATTC [GA]^AATT[CT]
Asp700I 3141 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 126 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 219 CACGTG CAC^GTG
BcgI 533 CGAGGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 53 GTATCC GTATCC(6/5)
BfaI 294 CTAG C^TAG
BfrI 2478 CTTAAG C^TTAAG
BglII 943 AGATCT A^GATCT
BsaAI 219 CACGTG [CT]AC^GT[GA]
BseAI 1635 TCCGGA T^CCGGA
BsiEI 929 CGGCCG CG[GA][CT]^CG
BsmI 2080 GCATTC GAATGC(1/-1)
BspEI 1635 TCCGGA T^CCGGA
BstBI 757 TTCGAA TT^CGAA
BstUI 1811 CGCG CG^CG
DraI 4521 TTTAAA TTT^AAA
DraIII 2251 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 929 CGGCCG C^GGCCG
Eam1105I 126 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 929 CGGCCG C^GGCCG
Eco52I 929 CGGCCG C^GGCCG
EcoRV 62 GATATC GAT^ATC
HaeII 2617 AGCGCC [GA]GCGC^[CT]
HincII 75 GTCAAC GT[CT]^[GA]AC
HindII 75 GTCAAC GT[CT]^[GA]AC
HindIII 4445 AAGCTT A^AGCTT
KspI 1810 CCGCGG CCGC^GG
MaeI 294 CTAG C^TAG
MfeI 2461 CAATTG C^AATTG
MroI 1635 TCCGGA T^CCGGA
MunI 2461 CAATTG C^AATTG
MvnI 1811 CGCG CG^CG
NspV 757 TTCGAA TT^CGAA
PmaCI 219 CACGTG CAC^GTG
PmlI 219 CACGTG CAC^GTG
PshAI 3264 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1810 CCGCGG CCGC^GG
SapI 158 GCTCTTC GCTCTTC(1/4)
SfuI 757 TTCGAA TT^CGAA
SspI 4514 AATATT AAT^ATT
XmnI 3141 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2527 GGTACC G^GTACC
4122 GGTACC
AccI 492 GTCTAC GT^[AC][GT]AC
1966 GTCTAC
AflIII 203 ACGTGT A^C[GA][CT]GT
3452 ACACGT
Alw44I 216 GTGCAC G^TGCAC
345 GTGCAC
ApaLI 216 GTGCAC G^TGCAC
345 GTGCAC
Asp718I 2527 GGTACC G^GTACC
4122 GGTACC
AspI 2496 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3436 GACCCGGTC
BalI 4304 TGGCCA TGG^CCA
4439 TGGCCA
BlpI 232 GCTCAGC GC^T[ACGT]AGC
306 GCTGAGC
Bpu1102I 232 GCTCAGC GC^T[ACGT]AGC
306 GCTGAGC
BspMI 4403 ACCTGC ACCTGC(4/8)
341 GCAGGT
BsrBI 87 GAGCGG CCGCTC(-3/-3)
693 GAGCGG
BstXI 3973 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4336 CCATGCCGATGG
CelII 232 GCTCAGC GC^T[ACGT]AGC
306 GCTGAGC
DrdI 645 GACAGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3664 GACCTCCTGGTC
EarI 159 CTCTTC CTCTTC(1/4)
2724 GAAGAG
EcoNI 335 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1044 CCTGGGATAGG
FseI 930 GGCCGGCC GGCCGG^CC
3387 GGCCGGCC
KpnI 2527 GGTACC GGTAC^C
4122 GGTACC
Ksp632I 159 CTCTTC CTCTTC(1/4)
2724 GAAGAG
MluNI 4304 TGGCCA TGG^CCA
4439 TGGCCA
MscI 4304 TGGCCA TGG^CCA
4439 TGGCCA
NspI 2335 GCATGC [GA]CATG^[CT]
3736 GCATGC
PflFI 2496 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3436 GACCCGGTC
SbfI 1859 CCTGCAGG CCTGCA^GG
3792 CCTGCAGG
SphI 2335 GCATGC GCATG^C
3736 GCATGC
Sse8387I 1859 CCTGCAGG CCTGCA^GG
3792 CCTGCAGG
Tth111I 2496 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3436 GACCCGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfiI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI